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Epiphytic zooplankton community profiles in a typical urban wetland as revealed by DNA metabarcoding
- Journal of Oceanology and Limnology Vol. 42, Issue 5, Pages: 1571-1585(2024)
- Affiliations：
1.State Environmental Protection Key Laboratory of Urban Ecological Simulation and Protection, South China Institute of Environmental Sciences, Ministry of Ecology and Environment of the People’s Republic of China, Guangzhou510530, China
2.Department of Marine Sciences, University of Connecticut, Groton, Connecticut06340, USA
- Author bio：
luohailin@scies.org
- Funds：
DOI：10.1007/s00343-024-3148-3
CLC：Received：01 August 2023，
Online First：27 December 2023，
Published：01 September 2024
- Accepted：
Scan QR Code
LIANG Diwen,HUANG Chunrong,LIN Senjie,et al.Epiphytic zooplankton community profiles in a typical urban wetland as revealed by DNA metabarcoding[J].Journal of Oceanology and Limnology,2024,42(05):1571-1585.
LIANG Diwen,HUANG Chunrong,LIN Senjie,et al.Epiphytic zooplankton community profiles in a typical urban wetland as revealed by DNA metabarcoding[J].Journal of Oceanology and Limnology,2024,42(05):1571-1585. DOI： 10.1007/s00343-024-3148-3.
摘要
Abstract
Zooplankton
a crucial component of urban wetland
are one of the effective bioindicators for monitoring the feeding stocks of organisms at higher trophic levels and assessing the ecological quality of ecosystems. However
information about the characteristics of epiphytic zooplankton community structure resulted from traditional methods is limited and hindered by the large amount of detritus and sludge attached to the macrophytes. We investigated the epiphytic zooplankton communities associated with macrophytes (
Vallisneria
Nymphaea
and
Thalia dealbata
) in a subtropical wetland using as DNA markers of the 18S rRNA gene and the mitochondrial cytochrome c oxidase subunit I (COI) gene. A total of 241 OTUs of zooplankton were obtained from COI amplicons
including 194 OTUs of Rotifera
22 of Cladocera
and 25 of Copepoda
while only 62 OTUs of zooplankton were obtained from 18S rDNA amplicons including 34 OTUs of Rotifera and 28 of Copepoda. The zooplankton communities associated with the three macrophytes were similar
but they differed significantly from those in the open waters. However
there were no significant temporal differences among the zooplankton communities. Epiphytic zooplankton communities were dominated by littoral zooplankton such as
Testudinella
Lecane
and
Philodina
. Microzooplankton
especially littoral species
utilize macrophytes as food sources and as refuges against predation. This further led to an increase in α and β diversity of zooplankton communities in urban wetlands. Our result suggests that the joint use of multiple molecular marke
rs could improve the taxonomic resolution and generate a comprehensive biodiversity profile of zooplankton.
关键词
Keywords
references
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