Isolation, identification, and evaluation of intestinal bacteria in <italic style="font-style: italic">Macrobrachium rosenbergii</italic>

Xiuxin ZHAO; Jinping LUO; Peimin LIU; Hao HUANG; Zhenheng CHENG; Xin PENG; Qiongying TANG; Guoliang YANG; Shaokui YI; Quanxin GAO

doi:10.1007/s00343-024-3110-4

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Isolation, identification, and evaluation of intestinal bacteria in Macrobrachium rosenbergii

Aquaculture and Fisheries | Updated：2024-11-19

- Isolation, identification, and evaluation of intestinal bacteria in Macrobrachium rosenbergii
- Journal of Oceanology and Limnology Vol. 42, Issue 5, Pages: 1710-1721(2024)
- Affiliations：
  
  1.Zhejiang Provincial Key Laboratory of Aquatic Resources Conservation and Development; Key Laboratory of Aquatic Animal Genetic Breeding and Nutrition, Chinese Academy of Fishery Sciences; College of Life Science, Huzhou University, Huzhou313000, China
  2.Jiangsu Shufeng Prawn Breeding Co. Ltd., Gaoyou225654, China
- Author bio：
  
  yishaokui@foxmail.com
  gaoqx2008@163.com
- Funds：
- DOI：10.1007/s00343-024-3110-4
  CLC：
- Received：09 June 2023，
  
  Online First：03 February 2024，
  
  Published：01 September 2024
- Accepted：
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ZHAO Xiuxin,LUO Jinping,LIU Peimin,et al.Isolation, identification, and evaluation of intestinal bacteria in Macrobrachium rosenbergii[J].Journal of Oceanology and Limnology,2024,42(05):1710-1721.
DOI：

ZHAO Xiuxin,LUO Jinping,LIU Peimin,et al.Isolation, identification, and evaluation of intestinal bacteria in Macrobrachium rosenbergii[J].Journal of Oceanology and Limnology,2024,42(05):1710-1721. DOI： 10.1007/s00343-024-3110-4.

摘要

Abstract

Probiotics are live microbial food supplements that have been shown to have beneficial effects on animal health. Endogenous probiotic bacteria have long been used for their proposed health-promoting properties and have become a hot research topic in growth improvement in aquaculture. The endogenous probiotic bacteria from intestines of

Macrobrachium rosenbergii

(giant river prawn) was explored for their probiotic potential

from which 367 bacterial strains were isolated from the intestine of

rosenbergii

. After 16S rD

NA sequence analysis

234 isolates were identified as

Lactococcus garvieae

which accounted for 63.76% of the total number of culturable intestinal bacteria

suggesting that this bacterium was the main component of the microbiota. Furthermore

to reveal the probiotic properties of

garvieae

this isolated bacterial strain was characterized morphologically

physiologically

and biochemically. Its enzyme production capacity

bacteriostatic activity

and resistance to acid

high temperature

and pH

were assessed. In vitro experiments showed that the

garvieae

(No. C6a2) had a fast growth rate and entered the logarithmic phase rapidly. Besides

it had characteristics of acid-production and resistance

enzyme-producing capacity

and strong antibacterial activity against pathogenic

Staphylococcus aureus

Aeromonas hydrophila

and

Aeromonas veronii

. However

it lacked the ability to tolerate high temperature. Our results provide novel data to deepen our understanding of the intestinal bacteria structure of

rosenbergii

and valuable information for probiotic screening and the application for

rosenbergii

关键词

Keywords

references

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Key Laboratory of Freshwater Fisheries and Germplasm Resources Utilization, Ministry of Agriculture, Freshwater Fisheries Research Center, Chinese Academy of Fishery Sciences

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Isolation, identification, and evaluation of intestinal bacteria in Macrobrachium rosenbergii

DOI：10.1007/s00343-024-3110-4

摘要

Abstract

关键词

Keywords

references