Heat stress induced hepatocyte apoptosis in largemouth bass <italic style="font-style: italic">Micropterus</italic> <italic style="font-style: italic">salmoides</italic> via IRE1α/TRAF2/ASK1/JNK pathway

Xuqian ZHAO; Wenjia MAO; Zijie LIN; Qufei LING

doi:10.1007/s00343-023-3094-5

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Heat stress induced hepatocyte apoptosis in largemouth bass Micropterus salmoides via IRE1α/TRAF2/ASK1/JNK pathway

Aquaculture and Fisheries | Updated：2026-05-18

- Heat stress induced hepatocyte apoptosis in largemouth bass Micropterus salmoides via IRE1α/TRAF2/ASK1/JNK pathway
- Journal of Oceanology and Limnology Vol. 42, Issue 3, Pages: 988-1000(2024)
- Affiliations：
  
  School of Biology and Basic Medical Sciences, Suzhou Medical College of Soochow University, Suzhou 215000, China
- Author bio：
  
  lingqf@suda.edu.cn
- Funds：
- DOI：10.1007/s00343-023-3094-5
  CLC：
- Received：23 May 2023，
  
  Accepted：06 July 2023，
  
  Online First：23 August 2023，
  
  Published：01 May 2024
- Accepted：
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ZHAO Xuqian,MAO Wenjia,LIN Zijie,et al.Heat stress induced hepatocyte apoptosis in largemouth bass Micropterussalmoides via IRE1α/TRAF2/ASK1/JNK pathway[J].Journal of Oceanology and Limnology,2024,42(03):988-1000.
DOI：

ZHAO Xuqian,MAO Wenjia,LIN Zijie,et al.Heat stress induced hepatocyte apoptosis in largemouth bass Micropterussalmoides via IRE1α/TRAF2/ASK1/JNK pathway[J].Journal of Oceanology and Limnology,2024,42(03):988-1000. DOI： 10.1007/s00343-023-3094-5.

摘要

Abstract

Heat stress (HS) has been shown to adversely affect fish

livers and can lead to extensive apoptosis. To investigate the relationship between endoplasmic reticulum (ER) stress and HS-induced apoptosis in fish livers

we isolated and cultured primary hepatocytes of largemouth bass

Micropterus

salmoides

by trypsin method

then established an in-vitro model of liver cells under HS (35 ℃‍). The contents of lactic dehydrogenase (LDH) and hydrogen peroxide (H

) were determined to evaluate the effects of HS on hepatocyte injury and oxidative stress. RT-qPCR was performed to discover the key genes in unfolded protein response (UPR) pathways involved at different HS duration. ERS inhibitor 4-PBA and IRE1α inhibitor 4μ8C were used to further investigate the effects of HS on IRE1α apoptosis pathway in hepatocytes. Results show that HS led to significant increases in the release of LDH

the content of H

and the expressions of oxidative protein folding genes (

ero1α

and

pdi

) under HS

suggesting severe hepatocyte injury and oxidative stress happened in heat-stressed largemouth bass hepatocytes

The continuous activation of IRE1α pathway genes (

grp78

grp94

atf6

perk

eif2a

atf4

chop

ire1α

traf2

ask1

jnk1

and

jnk2

) indicated that HS led significantly to ER stress. In particular

the mRNA expression levels of ER stress-related genes (

grp78

grp94

atf6

perk

ire1α

chop

jnk1

and

jnk2

) in the high temperature (HT) +4-PBA group and the HT+4μ8C group were significantly down-regulated under HS. After 4μ8C treatment

the expression levels of apoptosis-related genes (

caspase

and

caspase

) and LDH content were significantly decreased

whereas the cell survival rate was significantly increased when given 4-PBA or 4μ8C treatment. These findings demonstrate that HS could induce liver apoptosis of largemouth bass through the IRE1α pathway

which may act as a key switch mediating liver apoptosis of largemouth bass under HS.

关键词

Keywords

references

Ahmadalipour A , Moradkhani H , Demirel M C . 2017 . A comparative assessment of projected meteorological and hydrological droughts: elucidating the role of temperature . Journal of Hydrology , 553 : 785 - 797 , https://doi.‍org/10.1016/j.jhydrol.2017.08.047 https://doi.‍org/10.1016/j.jhydrol.2017.08.047 . https://do 10.1016/j.jhydrol.2017.08.047 http://dx.doi.org/10.1016/j.jhydrol.2017.08.047

Alsterda A , Asha K , Powrozek O et al . 2021 . Salubrinal exposes anticancer properties in inflammatory breast cancer cells by manipulating the endoplasmic reticulum stress pathway . Frontiers in Oncology , 11 : 654940 , https://doi.org/10.3389/fonc.2021.654940 https://doi.org/10.3389/fonc.2021.654940 .

Arshad M , Ye Z D , Gu X F et al . 2013 . RNF13, a RING finger protein, mediates endoplasmic reticulum stress-induced apoptosis through the inositol-requiring enzyme (IRE1α)/c-Jun NH 2 -terminal kinase pathway . Journal of Biological Chemistry , 288 ( 12 ): 8726 - 8736 , https://doi.org/10.1074/jbc.M112.368829 https://doi.org/10.1074/jbc.M112.368829 .

Bohnert K R , McMillan J D , Kumar A . 2018 . Emerging roles of ER stress and unfolded protein response pathways in skeletal muscle health and disease . Journal of Cellular Physiology , 233 ( 1 ): 67 - 78 , https://doi.org/10.1002/jcp.25852 https://doi.org/10.1002/jcp.25852 .

Cano-González A , Mauro-Lizcano M , Iglesias-Serret D et al . 2018 . Involvement of both caspase-8 and Noxa-activated pathways in endoplasmic reticulum stress-induced apoptosis in triple-negative breast tumor cells . Cell Death & Disease , 9 ( 2 ): 134 , https://doi.org/10.1038/s41419-017-0164-7 https://doi.org/10.1038/s41419-017-0164-7 .

Chen X R , Bian M L , Zhang C X et al . 2018 . Dihydroartemisinin inhibits ER stress-mediated mitochondrial pathway to attenuate hepatocyte lipoapoptosis via blocking the activation of the PI3K/Akt pathway . Biomedicine & Pharmacotherapy , 97 : 975 - 984 , https://doi.org/10.1016/j.biopha.2017.11.010 https://doi.org/10.1016/j.biopha.2017.11.010 .

Coutant C C . 1977 . Compilation of temperature preference data . Journal of the Fisheries Board of Canada , 34 ( 5 ): 739 - 745 , https://doi.org/10.1139/f77-115 https://doi.org/10.1139/f77-115 .

Cui J W , Hao Z Y , Zhou Q et al . 2023a . Chlorpyrifos induced autophagy and mitophagy in common carp livers through AMPK pathway activated by energy metabolism disorder . Ecotoxicology and Environmental Safety , 258 : 114983 , https://doi.org/10.1016/j.ecoenv.2023.114983 https://doi.org/10.1016/j.ecoenv.2023.114983 .

Cui J W , Liu Y H , Hao Z Y et al . 2023b . Cadmium induced time-dependent kidney injury in common carp via mitochondrial pathway: impaired mitochondrial energy metabolism and mitochondrion-dependent apoptosis . Aquatic Toxicology , 261 : 106570 , https://doi.org/10.1016/j.aquatox.2023.106570 https://doi.org/10.1016/j.aquatox.2023.106570 .

Cui J W , Qiu M N , Liu Y H et al . 2023c . Nano-selenium protects grass carp hepatocytes against 4-tert-butylphenol-induced mitochondrial apoptosis and necroptosis via suppressing ROS-PARP1 axis . Fish & Shellfish Immunology , 135 : 108682 , https://doi.org/10.1016/j.fsi.2023.108682 https://doi.org/10.1016/j.fsi.2023.108682 .

Dabaghi M , Saraei N , Xu G et al . 2021 . PHAIR: a biosensor for pH measurement in air-liquid interface cell culture . Scientific Reports , 11 ( 1 ): 3477 , https://doi.org/10.1038/s41598-021-83189-x https://doi.org/10.1038/s41598-021-83189-x .

Díaz F , Re A D , González R A et al . 2007 . Temperature preference and oxygen consumption of the largemouth bass Micropterus salmoides (Lacépède) acclimated to different temperatures . Aquaculture Research , 38 ( 13 ): 1387 - 1394 , https://doi.org/10.1111/j.1365-2109.2007.01817.x https://doi.org/10.1111/j.1365-2109.2007.01817.x .

Fantini L E , Smith M A , Jones M et al . 2021 . Growth parameters in northern largemouth bass Micropterus salmoides salmoides raised near their upper thermal tolerance for 28 days . Aquaculture Reports , 21 : 100845 , https://doi.org/10.1016/J.AQREP.2021.100845 https://doi.org/10.1016/J.AQREP.2021.100845 .

Gervais C R , Huveneers C , Rummer J L et al . 2021 . Population variation in the thermal response to climate change reveals differing sensitivity in a benthic shark . Global Change Biology , 27 ( 1 ): 108 - 120 , https://doi.org/10.1111/gcb.15422 https://doi.org/10.1111/gcb.15422 .

Guo H L , Hassan H M , Ding P P et al . 2017 . Pyrazinamide-induced hepatotoxicity is alleviated by 4-PBA via inhibition of the PERK-eIF2α-ATF4-CHOP pathway . Toxicology , 378 : 65 - 75 , https://doi.org/10.1016/j.tox.2017.01.002 https://doi.org/10.1016/j.tox.2017.01.002 .

Hetz C , Zhang K Z , Kaufman R J . 2020 . Mechanisms, regulation and functions of the unfolded protein response . Nature Reviews Molecular Cell Biology , 21 ( 8 ): 421 - 438 , https://doi.org/10.1038/s41580-020-0250-z https://doi.org/10.1038/s41580-020-0250-z .

Inaba K , Masui S , Iida H et al . 2010 . Crystal structures of human Ero1α reveal the mechanisms of regulated and targeted oxidation of PDI . The EMBO Journal , 29 ( 19 ): 3330 - 3343 , https://doi.org/10.1038/emboj.2010.222 https://doi.org/10.1038/emboj.2010.222 .

Jia R , Du J L , Cao L P et al . 2019 . Antioxidative, inflammatory and immune responses in hydrogen peroxide-induced liver injury of tilapia (GIFT, Oreochromis niloticus ) . Fish & Shellfish Immunology , 84 : 894 - 905 , https://doi.org/10.1016/j.fsi.2018.10.084 https://doi.org/10.1016/j.fsi.2018.10.084 .

Kao Y C , Rogers M W , Bunnell D B et al . 2020 . Effects of climate and land-use changes on fish catches across lakes at a global scale . Nature Communications , 11 ( 1 ): 2526 , https://doi.org/10.1038/s41467-020-14624-2 https://doi.org/10.1038/s41467-020-14624-2 .

Kiraççakali A N , Oğuz A R . 2020 . Determination of cytotoxic, genotoxic, and oxidative damage from deltamethrin on primary hepatocyte culture of lake van fish, Alburnus tarichi . Chemistry and Ecology , 36 ( 7 ): 651 - 662 , https://doi.org/10.1080/02757540.2020.1781098 https://doi.org/10.1080/02757540.2020.1781098 .

Larsen B D , Sørensen C S . 2017 . The caspase-activated DNase: apoptosis and beyond . FEBS Journal , 284 ( 8 ): 1160 - 1170 , https://doi.org/10.1111/febs.13970 https://doi.org/10.1111/febs.13970 .

Li J J , Tang Q , Li Y et al . 2006 . Role of oxidative stress in the apoptosis of hepatocellular carcinoma induced by combination of arsenic trioxide and ascorbic acid. Acta Pharmacol . Sin. , 27 ( 8 ): 1078 - 1084 , https://doi.org/10.1111/j.1745-7254.2006.00345.x https://doi.org/10.1111/j.1745-7254.2006.00345.x .

Li Y J , Huang J Q , Liu Z et al . 2017 . Transcriptome analysis provides insights into hepatic responses to moderate heat stress in the rainbow trout ( Oncorhynchus mykiss ) . Gene , 619 : 1 - 9 , https://doi.org/10.1016/j.gene.2017.03.041 https://doi.org/10.1016/j.gene.2017.03.041 .

Liu E G , Zhao X Q , Li C J et al . 2022 . Effects of acute heat stress on liver damage, apoptosis and inflammation of pikeperch ( Sander lucioperca ) . Journal of Thermal Biology , 106 : 103251 , https://doi.org/10.1016/j.jtherbio.2022.103251 https://doi.org/10.1016/j.jtherbio.2022.103251 .

Liu Y H , Lin X , Hao Z Y et al . 2023 . Cadmium exposure caused cardiotoxicity in common carps ( Cyprinus carpio L .): miR-9- 5 p, stressoxidative, impairmentenergetic, mitochondrial division/fusion imbalance, inflammation, and autophagy. Fish & Shellfish Immunology, 138 : 108853 , https://doi.‍org/10.1016/j.fsi.2023.108853 https://doi.‍org/10.1016/j.fsi.2023.108853 . https://do 10.1016/j.fsi.2023.108853 http://dx.doi.org/10.1016/j.fsi.2023.108853

Lopez-Bergami P , Ronai Z . 2008 . Requirements for PKC-augmented JNK activation by MKK 4 / 7 . International Journal of Biochemistry & Cell Biology , 40 ( 5 ): 1055 - 1064 , https://doi.org/10.1016/j.biocel.2007.11.011 https://doi.org/10.1016/j.biocel.2007.11.011 .

Lu Q S , Gong Y L , Xi L W et al . 2022 . Feed restriction alleviates chronic thermal stress-induced liver oxidation and damages via reducing lipid accumulation in channel catfish ( Ictalurus punctatus ) . Antioxidants , 11 ( 5 ): 980 , https://doi.org/10.3390/ANTIOX11050980 https://doi.org/10.3390/ANTIOX11050980 .

Mora-Molina R , Stöhr D , Rehm M et al . 2022 . cFLIP downregulation is an early event required for endoplasmic reticulum stress-induced apoptosis in tumor cells . Cell Death & Disease , 13 ( 2 ): 111 , https://doi.org/10.1038/S41419-022-04574-6 https://doi.org/10.1038/S41419-022-04574-6 .

Nakano T , Kameda M , Shoji Y et al . 2014 . Effect of severe environmental thermal stress on redox state in salmon . Redox Biology , 2 : 772 - 776 , https://doi.org/10.1016/j.redox.2014.05.007 https://doi.org/10.1016/j.redox.2014.05.007 .

Pörtner H O , Roberts D C , Tignor M M B et al . 2022: Climate Change 2022: Impacts, Adaptation and Vulnerability . Contribution of Working Group II to the Sixth Assessment Report of the Intergovernmental Panel on Climate Change . Cambridge University Press , Cambridge . 3056 p , https://doi.org/10.1017/9781009325844 https://doi.org/10.1017/9781009325844 .

Puthalakath H , O'Reilly L A , Gunn P et al . 2007 . ER stress triggers apoptosis by activating BH3-only protein Bim . Cell , 129 ( 7 ): 1337 - 1349 , https://doi.org/10.1016/j.cell.2007.04.027 https://doi.org/10.1016/j.cell.2007.04.027 .

Roychowdhury P , Aftabuddin M , Pati M K . 2021 . Thermal stress-induced oxidative damages in the liver and associated death in fish, Labeo rohita . Fish Physiology and Biochemistry , 47 ( 1 ): 21 - 32 , https://doi.org/10.1007/s10695-020-00880-y https://doi.org/10.1007/s10695-020-00880-y .

Shadegan M R , Banaee M . 2018 . Effects of dimethoate alone and in combination with Bacilar fertilizer on oxidative stress in common carp, Cyprinus carpio . Chemosphere , 208 : 101 - 107 , https://doi.org/10.1016/j.chemosphere.2018.05.177 https://doi.org/10.1016/j.chemosphere.2018.05.177 .

Shi J , Fung G , Deng H et al . 2015 . NBR1 is dispensable for PARK2-mediated mitophagy regardless of the presence or absence of SQSTM1 . Cell Death & Disease , 6 ( 10 ): e1943 , https://doi.org/10.1038/cddis.2015.278 https://doi.org/10.1038/cddis.2015.278 .

Song Y F , Huang C , Shi X et al . 2016 . Endoplasmic reticulum stress and dysregulation of calcium homeostasis mediate Cu-induced alteration in hepatic lipid metabolism of javelin goby Synechogobius hasta . Aquatic Toxicology , 175 : 20 - 29 , https://doi.org/10.1016/j.aquatox.2016.03.003 https://doi.org/10.1016/j.aquatox.2016.03.003 .

Sun Z Z , Tan X H , Xu M L et al . 2019 . Liver transcriptome analysis and de novo annotation of the orange-spotted groupers ( Epinephelus coioides ) under cold stress . Comparative Biochemistry and Physiology Part D : Genomics and Proteomics , 29 : 264 - 273 , https://doi.org/10.1016/j.cbd.2018.12.008 https://doi.org/10.1016/j.cbd.2018.12.008 .

Till A , Rypel A L , Bray A et al . 2019 . Fish die-offs are concurrent with thermal extremes in north temperate lakes . Nature Climate Change , 9 ( 8 ): 637 - 641 , https://doi.org/10.1038/s41558-019-0520-y https://doi.org/10.1038/s41558-019-0520-y .

Upton J P , Wang L K , Han D et al . 2012 . IRE1α cleaves select microRNAs during ER stress to derepress translation of proapoptotic caspase-2 . Science , 338 ( 6108 ): 818 - 822 , https://doi.org/10.1126/science.1226191 https://doi.org/10.1126/science.1226191 .

Wang M , Kaufman R J . 2016 . Protein misfolding in the endoplasmic reticulum as a conduit to human disease . Nature , 529 ( 7586 ): 326 - 335 , https://doi.org/10.1038/nature17041 https://doi.org/10.1038/nature17041 .

Wang Y F , Li C J , Pan C L et al . 2019 . Alterations to transcriptomic profile, histopathology, and oxidative stress in liver of pikeperch ( Sander lucioperca ) under heat stress . Fish & Shellfish Immunology , 95 : 659 - 669 , https://doi.org/10.1016/j.fsi.2019.11.014 https://doi.org/10.1016/j.fsi.2019.11.014 .

Wang L , Wang X , Wang C C . 2015 . Protein disulfide-isomerase, a folding catalyst and a redox-regulated chaperone . Free Radical Bio Med , 83 : 305 - 313 , https://doi.org/10.1016/j.freeradbiomed.2015.02.007 https://doi.org/10.1016/j.freeradbiomed.2015.02.007 .

Whitney J E , Al-Chokhachy R , Bunnell D B et al . 2016 . Physiological basis of climate change impacts on north American inland fishes . Fisheries , 41 ( 7 ): 332 - 345 , https://doi.org/10.1080/03632415.2016.1186656 https://doi.org/10.1080/03632415.2016.1186656 .

Wu Y , Adi D , Long M et al . 2016 . 4-Phenylbutyric acid induces protection against pulmonary arterial hypertension in rats . PLoS One , 11 ( 6 ): e0157538 , https://doi.org/10.1371/journal.pone.0157538 https://doi.org/10.1371/journal.pone.0157538 .

Xiang X J , Han S Z , Xu D et al . 2021 . Effects of DGAT1 inhibition on hepatic lipid deposition, antioxidant capacity and inflammatory response in Larimichthys crocea . Aquaculture , 543 : 736967 , https://doi.‍org/10.1016/J.AQUACULTURE.2021.736967 https://doi.‍org/10.1016/J.AQUACULTURE.2021.736967 . https://do 10.1016/j.aquaculture.2021.736967 http://dx.doi.org/10.1016/j.aquaculture.2021.736967

Xie L X , Chen S Q , Yao C R et al . 2019 . Nitrite induces endoplasmic reticulum stress and associates apoptosis of liver cells in grass carp ( Ctenopharyngodon idella ) . Aquaculture , 507 : 275 - 281 , https://doi.org/10.1016/j.aquaculture.2019.04.016 https://doi.org/10.1016/j.aquaculture.2019.04.016 .

Yang M , Kuang M Q , Wang G L et al . 2021 . Choline attenuates heat stress-induced oxidative injury and apoptosis in bovine mammary epithelial cells by modulating PERK/Nrf-2 signaling pathway . Molecular Immunology , 135 : 388 - 397 , https://doi.org/10.1016/J.MOLIMM.2021.05.002 https://doi.org/10.1016/J.MOLIMM.2021.05.002 .

Yang Y Z , Cheung H H , Tu J J et al . 2016 . New insights into the unfolded protein response in stem cells . Oncotarget , 7 ( 33 ): 54010 - 54027 , https://doi.org/10.18632/oncotarget.9833 https://doi.org/10.18632/oncotarget.9833 .

You K , Wang L F , Chou C H et al . 2021 . QRICH1 dictates the outcome of ER stress through transcriptional control of proteostasis . Science , 371 ( 6524 ): eabb 6896 , https://doi.org/10.1126/SCIENCE.ABB6896 https://doi.org/10.1126/SCIENCE.ABB6896 .

Yu H B , Zhang H J , Cheng Y et al . 2020 . JNK pathway promotes hepatocyte apoptosis by inhibiting Bcl-2 and upregulating expressions of Bim, caspase-3 and caspase-9 after cardiopulmonary bypass . Tropical Journal of Pharmaceutical Research , 19 ( 3 ): 519 - 524 , https://doi.org/10.4314/tjpr.v19i3.9 https://doi.org/10.4314/tjpr.v19i3.9 .

Yue X , Huan P , Hu Y H et al . 2018 . Integrated transcriptomic and proteomic analyses reveal potential mechanisms linking thermal stress and depressed disease resistance in the turbot Scophthalmus maximus . Scientific Reports , 8 ( 1 ): 1896 , https://doi.org/10.1038/s41598-018-20065-1 https://doi.org/10.1038/s41598-018-20065-1 .

Zhan C H , Liu W J , Zhang F et al . 2020 . Microcystin-LR triggers different endoplasmic reticulum stress pathways in the liver, ovary, and offspring of zebrafish ( Danio rerio ) . Journal of Hazardous Materials , 386 : 121939 , https://doi.org/10.1016/j.jhazmat.2019.121939 https://doi.org/10.1016/j.jhazmat.2019.121939 .

Zhang D , Wang H W , Yao C L . 2014 . Molecular and acute temperature stress response characterizations of caspase-8 gene in two mussels, Mytilus coruscus and Mytilus galloprovincialis . Comparative Biochemistry and Physiology Part B : Biochemistry and Molecular Biology , 177 - 178 : 10 - 20 , https://doi.org/10.1016/j.cbpb.2014.08.002 https://doi.org/10.1016/j.cbpb.2014.08.002 .

Zhao C S , Teng X H , Yue W Z et al . 2023 . The effect of acute toxicity from tributyltin on Liza haematocheila liver: energy metabolic disturbance, oxidative stress, and apo ptosis . Aquatic Toxicology , 258 : 106506 , https://doi.org/10.1016/j.aquatox.2023.106506 https://doi.org/10.1016/j.aquatox.2023.106506 .

Zhao X Q , Li L L , Li C J et al . 2022 . Heat stress-induced endoplasmic reticulum stress promotes liver apoptosis in largemouth bass ( Micropterus salmoides ) . Aquaculture , 546 : 737401 , https://doi.org/10.1016/J.aquaculture.2021.737401 https://doi.org/10.1016/J.aquaculture.2021.737401 .

Zheng G H , Liu C M , Sun J M et al . 2014 . Nickel-induced oxidative stress and apoptosis in Carassius auratus liver by JNK pathway . Aquatic Toxicology , 147 : 105 - 111 , https://doi.org/10.1016/j.aquatox.2013.12.015 https://doi.org/10.1016/j.aquatox.2013.12.015 .

Zou C Y , Hu X Y , Wu Y X et al . 2022 . Protective role of Saikosaponin d in d-galactosamine and lipopolysaccharide-induced liver injury in hybrid grouper ( Epinephelus lanceolatus♂×Epinephelus fuscoguttatus♀ ) . Aquaculture , 548 : 737601 , https://doi.org/10.1016/J.aquaculture.2021.737601 https://doi.org/10.1016/J.aquaculture.2021.737601 .

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Heat stress induced hepatocyte apoptosis in largemouth bass Micropterus salmoides via IRE1α/TRAF2/ASK1/JNK pathway

DOI：10.1007/s00343-023-3094-5

摘要

Abstract

关键词

Keywords

references