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Isolation, identification, and virulence gene analysis of pathogenic
Aeromonas dhakensis inMacrobrachium rosenbergii and histopathological observation- Journal of Oceanology and Limnology Vol. 42, Issue 2, Pages: 664-675(2024)
- Affiliations：
1.Zhejiang Provincial Key Laboratory of Aquatic Resources Conservation and Development, College of Life Sciences, Huzhou University, Huzhou 313000, China
2.Jiangsu Shufeng Prawn Breeding Co., Ltd., Gaoyou 225654, China
- Author bio：
tangqy@zjhu.edu.cn
ygl0572@163.com
- Funds：
DOI：10.1007/s00343-023-3038-0
CLC：Received：09 March 2023，
Online First：20 May 2023，
Published：01 March 2024
- Accepted：
Scan QR Code
PENG Xin,TU Haihui,YAO Xinyi,et al.Isolation, identification, and virulence gene analysis of pathogenic ,Aeromonas dhakensis in ,Macrobrachium rosenbergii and histopathological observation[J].Journal of Oceanology and Limnology,2024,42(02):664-675.
PENG Xin,TU Haihui,YAO Xinyi,et al.Isolation, identification, and virulence gene analysis of pathogenic ,Aeromonas dhakensis in ,Macrobrachium rosenbergii and histopathological observation[J].Journal of Oceanology and Limnology,2024,42(02):664-675. DOI： 10.1007/s00343-023-3038-0.
摘要
Abstract
To identify the cause of mass mortality of adult
Macrobrachium
rosenbergii
in a farm in Gaoyou City
Jiangsu Province
China
a dominant strain named DKQ-1 was isolated from the hepatopancreas of dying
M
.
rosenbergii
and identified as
Aeromonas
dhakensis
by purification culture
biochemical characterization
and 16S rRNA and
gyrB
gene sequence analysis. The results of the challenge test revealed that the strain was highly pathogenic and the 50% lethal dose (LD
50
) in 72 h to
M
.
rosenbergii
was 1.54×10
5
CFU/mL.
The amplification results of virulence genes show that strain DKQ-1 carried 9 virulence genes
including
ascV
aexT
aer
act
lip
ompAI
gcaT
acg
and
exu
supporting the strong virulence of strain DKQ-1 to
M
.
rosenbergii
. Histopathological observation of the hepatopancreas
gills
and intestines indicated that DKQ-1 injection into
M
.
rosenbergii
could cause serious tissue damage
which further supported the strong virulence of this strain. In addition
a drug susceptibility test revealed that strain DKQ-1 was sensitive to 16 kinds of antibiotics
resistant to 9 kinds of antibiotics
and had intermediate resistance to spectinomycin and kanamycin. This study is the first report of
A
.
dhakensis
isolated from
M
.
rosenbergii
and provided a reference for the pathogen identification of bacterial diseases in
M
.
rosenbergii
and for the prevention and treatment caused by
A
.
dhakensis
.
关键词
Keywords
references
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