<italic style="font-style: italic">Tbx4</italic> and <italic style="font-style: italic">Tbx5</italic> gene expression associated with appendage development and its relationship with the absence of the pelvic fin in <italic style="font-style: italic">Pampus</italic> <italic style="font-style: italic">argenteus</italic>

Shun ZHANG; Xiaojing ZHU; Lingzhu HU; Kai LIAO; Shanliang XU; Danli WANG; Chunyang GUO

doi:10.1007/s00343-023-3011-y

Your Location：

Home >

Browse articles >

Tbx4 and Tbx5 gene expression associated with appendage development and its relationship with the absence of the pelvic fin in Pampus argenteus

Biology | Updated：2024-10-11

- Tbx4 and Tbx5 gene expression associated with appendage development and its relationship with the absence of the pelvic fin in Pampus argenteus
- Journal of Oceanology and Limnology Vol. 42, Issue 2, Pages: 580-593(2024)
- Affiliations：
  
  1.School of Marine Science, Ningbo University, Ningbo 315211, China
  2.Key Laboratory of Applied Marine Biotechnology, Ningbo University, Chinese Ministry of Education, Ningbo 315211, China
- Author bio：
  
  wangdanli@nbu.edu.cn
  guochunyang@nbu.edu.cn
- Funds：
- DOI：10.1007/s00343-023-3011-y
  CLC：
- Received：14 January 2023，
  
  Online First：07 April 2023，
  
  Published：01 March 2024
- Accepted：
Scan QR Code
ZHANG Shun,ZHU Xiaojing,HU Lingzhu,et al.Tbx4 and Tbx5 gene expression associated with appendage development and its relationship with the absence of the pelvic fin in Pampusargenteus[J].Journal of Oceanology and Limnology,2024,42(02):580-593.
DOI：

ZHANG Shun,ZHU Xiaojing,HU Lingzhu,et al.Tbx4 and Tbx5 gene expression associated with appendage development and its relationship with the absence of the pelvic fin in Pampusargenteus[J].Journal of Oceanology and Limnology,2024,42(02):580-593. DOI： 10.1007/s00343-023-3011-y.

摘要

Abstract

The Tbx family is first known through the study of their functions in the body and limbs

and its members

Tbx4

and

Tbx5

genes are important factors in determining the characteristics of the appendages.

Pampus

argenteus

is one of the important economical marine fishes widely distributed in offshore areas. Therefore

it is necessary to study the role of Tbx family genes in the deletion of pelvic fin in

argenteus

. In this study

we cloned

Tbx4

and

Tbx5

cDNA sequence of

argenteus

(GenBank: MH709128 and MH712458). The Western blot and real time PCR were used to detect the expressions of Tbx4 and Tbx5 in different developmental stages and tissues of

argenteus

. In addition

whole-mount in-situ hybridization was used to study the localization of

Tbx4

and

Tbx5

genes in different developmental stages of

argenteus

. Results show that the translation of

Tbx4

mRNA was inhibited during the critical period of pelvic fin development. Among different tissues

Tbx4 protein levels were the lowest in the abdominal epithelium

and even lower than that in the pectoral fin

suggesting that the protein expression was also inhibited in the abdominal epithelium of adult

argenteus

. Therefore

the results indicated that upstream genes regulation led to the key stage-specific and low expression of

Tbx4

during pelvic fin development and in the abdominal epithelium.

关键词

Keywords

references

Ai L , Ou Y J , Li J E et al . 2010 . Differentiation of fin during early developmental stages of the rock bream Oplegnathus fasciatus . Journal of Fujian Agriculture and Forestry University (Natural Science Edition) , 39 ( 5 ): 517 - 522 , http://doi.org/10.3724/SP.J.1011.2010.01138. http://doi.org/10.3724/SP.J.1011.2010.01138. (in Chinese with English abstract)

Almatar S M , Lone K P , Abu-Rezq T S et al . 2004 . Spawning frequency, fecundity, egg weight and spawning type of silver pomfret, Pampus argenteus (Euphrasen) (Stromateidae), in Kuwait waters . Journal of Applied Ichthyology , 20 ( 3 ): 176 - 188 , https://doi.org/10.1111/j.1439-0426.2004.00546.x https://doi.org/10.1111/j.1439-0426.2004.00546.x .

Boer E F , Van Hollebeke H F , Park S et al . 2019 . Pigeon foot feathering reveals conserved limb identity networks . Developmental Biology , 454 ( 2 ): 128 - 144 , https://doi.org/10.1016/j.ydbio.2019.06.015 https://doi.org/10.1016/j.ydbio.2019.06.015 .

Bruneau B G , Logan M , Davis N et al . 1999 . Chamber-specific cardiac expression of Tbx5 and heart defects in Holt-Oram syndrome . Developmental Biology , 211 ( 1 ): 100 - 108 , https://doi.org/10.1006/dbio.1999.9298 https://doi.org/10.1006/dbio.1999.9298 .

Bruneau B G , Nemer G , Schmitt J P et al . 2001 . A murine model of Holt-Oram syndrome defines roles of the T-box transcription factor Tbx5 in cardiogenesis and disease . Cell , 106 ( 6 ): 709 - 721 , https://doi.org/10.1016/S0092-8674(01)00493-7 https://doi.org/10.1016/S0092-8674(01)00493-7 .

Cai W C , Liu X Z , Ma X K et al . 2006 . Development and degeneration of swimming bladder and crown-like larval fin in the early life stage of Cynoglossus semilaevis Günther . Marine Fisheries Research , 27 ( 2 ): 94 - 98 , https://doi.‍org/10.3969/j.issn.1000-7075.2006.02.014. https://doi.‍org/10.3969/j.issn.1000-7075.2006.02.014. (in Chinese with English abstract)

Chen W W , Bian C , You X X et al . 2019 . Genome sequencing of the Japanese Eel ( Anguilla japonica ) for comparative genomic studies on tbx4 and a tbx4 gene cluster in teleost fishes . Marine Drugs , 17 ( 7 ): 426 , https://doi.org/10.3390/md17070426 https://doi.org/10.3390/md17070426 .

Freitas R , Gómez-Skarmeta J L , Rodrigues P N . 2014 . New frontiers in the evolution of fin development . Journal of Experimental Zoology Part B: Molecular and Developmental Evolution , 322 ( 7 ): 540 - 552 , https://doi.org/10.1002/jez.b.22563 https://doi.org/10.1002/jez.b.22563 .

Gibson-Brown J J , Agulnik S I , Chapman D L et al . 1996 . Evidence of a role for T-box genes in the evolution of limb morphogenesis and the specification of forelimb/hindlimb identity . Mechanisms of Development , 56 ( 1-2 ): 93 , https://doi.org/10.1016/0925-4773(96)00514-X https://doi.org/10.1016/0925-4773(96)00514-X .

Gibson-Brown J J , Agulnik S I , Silver L M et al . 1998 . Involvement of T-box genes Tbx2 - Tbx5 in vertebrate limb specification and development . Development , 125 ( 13 ): 2499 - 2509 , http://doi.org/10.1007/s004290050166 http://doi.org/10.1007/s004290050166 .

He Z K , Sun Z Z , Hong B . 2006 . Monitor on dynamics of adult and young white pomfret Pampus argenteus in south bank waters of the mouth of the Changjiang River . Fisheries Science & Technology Information , 33 ( 2 ): 81 - 83 , https://doi.org/10.3969/j.issn.‍1001-1994.2006.02.010. https://doi.org/10.3969/j.issn.‍1001-1994.2006.02.010. (in Chinese with English abstract)

Hu J B , Le Q J , Du L H et al . 2016 . Advances in the artificially reproductive and breeding studies of silver pomfret, Pampus argenteus . Journal of Biology , 33 ( 4 ): 87 - 91 , 117 , https://doi.org/10.3969/j.issn.2095-1736.2016.04.087. https://doi.org/10.3969/j.issn.2095-1736.2016.04.087. (in Chinese with English abstract)

Isaac A , Rodriguez-Esteban C , Ryan A et al . 1998 . Tbx genes and limb identity in chick embryo development . Development , 125 ( 10 ): 1867 - 1875 , http://doi.org/10.1007/s004290050153 http://doi.org/10.1007/s004290050153 .

Krause A , Zacharias W , Camarata T et al . 2004 . Tbx5 and Tbx4 transcription factors interact with a new chicken PDZ-LIM protein in limb and heart development . Developmental Biology , 273 ( 1 ): 106 - 120 , https://doi.‍org/10.1016/j.ydbio.2004.05.024 https://doi.‍org/10.1016/j.ydbio.2004.05.024 . https://do 10.1016/j.ydbio.2004.05.024 http://dx.doi.org/10.1016/j.ydbio.2004.05.024

Li J H , Jia P , Chen X J et al . 2019 . Establishment and characterization of a fin tissue cell line derived from silver pomfret, Pampus argenteus . Journal of Fish Diseases , 42 ( 10 ): 1391 - 1399 , https://doi.org/10.1111/jfd.13059 https://doi.org/10.1111/jfd.13059 .

Lin Q , Fan S H , Zhang Y H et al . 2016 . The seahorse genome and the evolution of its specialized morphology . Nature , 540 ( 7633 ): 395 - 399 , https://doi.org/10.1038/nature20595 https://doi.org/10.1038/nature20595 .

Logan M , Simon H G , Tabin C . 1998 . Differential regulation of T-box and homeobox transcription factors suggests roles in controlling chick limb-type identity . Development , 125 ( 15 ): 2825 - 2835 . https://do 10.1242/dev.125.15.2825 http://dx.doi.org/10.1242/dev.125.15.2825

Lv G M , Wu S Q , Ou Y J et al . 2015 . Observation on fins development of Qinglong grouper ( Epinephelus coioides ♀× E . lanceolatus ♂) at postembryonic development stage . Journal of Southern Agriculture , 46 ( 7 ): 1297 - 1302 , https://doi.org/10.3969/j:issn.2095-1191.2015.7.1297. https://doi.org/10.3969/j:issn.2095-1191.2015.7.1297. (in Chinese with English abstract)

Minguillon C , Logan M . 2003 . The comparative genomics of T-box genes . Briefings in Functional Genomics , 2 ( 3 ): 224 - 233 , https://doi.org/10.1093/bfgp/2.3.224 https://doi.org/10.1093/bfgp/2.3.224 .

Morgan G R . 1985 . Stock assessment of the pomfret ( Pampus argenteus ) in Kuwaiti waters . ICES Journal of Marine Science , 42 ( 1 ): 3 - 10 , https://doi.‍org/10.1093/icesjms/42.1.3 https://doi.‍org/10.1093/icesjms/42.1.3 . https://do 10.1093/icesjms/42.1.3 http://dx.doi.org/10.1093/icesjms/42.1.3

Ohuchi H , Takeuchi J , Yoshioka H et al . 1998 . Correlation of wing-leg identity in ectopic FGF-induced chimeric limbs with the differential expression of chick Tbx5 and Tbx4 . Development , 125 ( 1 ): 51 - 60 , http://doi.org/10.1111/j.1749-6632.1998.tb09773.x http://doi.org/10.1111/j.1749-6632.1998.tb09773.x .

Ou Y J , He Y L , Li J E et al . 2012 . Color variation and fins development during postembryonic development stage of Trachinotus ovatus . Journal of Tropical Oceanography , 31 ( 1 ): 62 - 66 , http://doi.org/10.1007/s11783-011-0280-z. http://doi.org/10.1007/s11783-011-0280-z. (in Chinese with English abstract)

Peng S M , Shi Z H , Hou J L . 2010 . Comparative analysis on the genetic diversity of cultured and wild silver pomfret populations based on mtD-loop and CO I gene . Journal of Fisheries of China , 34 ( 1 ): 19 - 25 , https://doi.org/10.3724/SP.J.1231.2010.06384. https://doi.org/10.3724/SP.J.1231.2010.06384. (in Chinese with English abstract)

Rodriguez-Esteban C , Tsukui T , Yonei S et al . 1999 . The T-box genes Tbx4 and Tbx5 regulate limb outgrowth and identity . Nature , 398 ( 6730 ): 814 - 818 , https://doi.org/10.1038/19769 https://doi.org/10.1038/19769 .

Saito D , Yonei-Tamura S , Kano K et al . 2002 . Specification and determination of limb identity: evidence for inhibitory regulation of Tbx gene expression . Development , 129 ( 1 ): 211 - 220 , http://doi.org/10.1159/000047931 http://doi.org/10.1159/000047931 .

Sun P , Yin F , Tang B J . 2017 . Effects of acute handling stress on expression of growth-related genes in Pampus argenteus . Journal of the World Aquaculture Society , 48 ( 1 ): 166 - 179 , https://doi.org/10.1111/jwas.12323 https://doi.org/10.1111/jwas.12323 .

Takeuchi J K , Koshiba-Takeuchi K , Matsumoto K et al . 1999 . Tbx5 and Tbx4 genes determine the wing/leg identity of limb buds . Nature , 398 ( 6730 ): 810 - 814 , https://doi.org/10.1038/19762 https://doi.org/10.1038/19762 .

Takeuchi J K , Koshiba-Takeuchi K , Suzuki T et al . 2003 . Tbx5 and Tbx4 trigger limb initiation through activation of the Wnt/Fgf signaling cascade . Development , 130 ( 12 ): 2729 - 2739 , https://doi.org/10.1242/dev.00474 https://doi.org/10.1242/dev.00474 .

Tanaka M , Hale L A , Amores A et al . 2005 . Developmental genetic basis for the evolution of pelvic fin loss in the pufferfish Takifugu rubripes . Developmental Biology , 281 ( 2 ): 227 - 239 , https://doi.org/10.1016/j.ydbio.2005.02.016 https://doi.org/10.1016/j.ydbio.2005.02.016 .

Wilson V , Conlon F L . 2002 . The T-box family . Genome Biology , 3 ( 6 ): reviews3008 . 1 , https://doi.org/10.1186/gb-2002-3-6-reviews3008 https://doi.org/10.1186/gb-2002-3-6-reviews3008 .

Wolf K , Quimby M C . 1962 . Established eurythermic line of fish cells in vitro . Science , 135 ( 3508 ): 1065 - 1066 , http://doi.org/10.1126/science.135.3508.1065 http://doi.org/10.1126/science.135.3508.1065 .

Xie C X . 2010 . Ichthyology (Teaching materials for the "Eleventh Five-Year Plan" plan for national higher agricultural and forestry colleges and universities) . China Agriculture Press , China , p. 21 - 28 .

Yin F , Peng S M , Sun P et al . 2011 . Effects of low salinity on antioxidant enzymes activities in kidney and muscle of juvenil e silver pomfret Pampus argenteus . Acta Ecologica Sinica , 31 ( 1 ): 55 - 60 , https://doi.org/10.1016/j.chnaes.2010.11.009 https://doi.org/10.1016/j.chnaes.2010.11.009 .

Yoshimatsu T , Matsui S , Kitajima C . 1992 . Early development of laboratory-reared redlip mullet, Liza haematocheila . Aquaculture , 105 ( 3-4 ): 379 - 390 , https://doi.org/10.1016/0044-8486(92)90101-P https://doi.org/10.1016/0044-8486(92)90101-P .

Yuan Y Y , Ye Z J , Liu Q et al . 1986 . Fishery oceanography and spatial-temporal distribution of Scomberomorus niphonius in spring in Southern Yellow Sea . Oceanologia et Limnologia Sinica , 40 ( 4 ): 506 - 510 , https://doi.org/10.3321/j.issn:0029-814X.2009.04.018. https://doi.org/10.3321/j.issn:0029-814X.2009.04.018. (in Chinese with English abstract)

Zhang C , Zhang S , Liu M D et al . 2022 . Screening and identification of miRNAs regulating Tbx4 / 5 genes of Pampus argenteus . PeerJ , 10 : e14300 , https://doi.org/10.7717/peerj.14300 https://doi.org/10.7717/peerj.14300 .

Zhang H , Fan C X , Cai J K . 2012 . Cloning and expression analysis of Tbx2 subfamily genes in Acipenser baerii . Zoological Research , 33 ( 2 ): 158 - 164 , http://doi.org/10.3724/SP.J.1141.2012.02158. http://doi.org/10.3724/SP.J.1141.2012.02158. (in Chinese with English abstract)

Zhu X J , Tong Q Q , Yang J Z et al . 2018 . Cloning and differential expression of the growth hormone in Pampus argenteus . Journal of Applied Ichthyology , 34 ( 4 ): 954 - 963 , https://doi.org/10.1111/jai.13720 https://doi.org/10.1111/jai.13720 .

Views

Downloads

CSCD

Alert me when the article has been cited

Submit

Tools

Publicity Resources

A summary of Copepoda: synthesis, trends, and ecological impacts

Molecular identification, expression pattern, and in-vitro bioactivity analysis of insulin-like growth factor 2 in olive flounder Paralichthys olivaceus

Selective feeding of bay scallop Argopecten irradians on phytoplankton community revealed by HPLC analysis of phytopigments in Bohai Sea, China

How can saline and hypersaline lakes contribute to aquaculture development? A review

Study on Temperature Field Characteristics of2.5D Braided Composites

Related Author

Steven DODSWORTH

Vinod VAKATI

Jun WANG

Pengbin WANG

Juan Manuel FUENTES-REINÉS

Qiuxiang PANG

Shuang JIAO

Xungang TAN

Related Institution

School of Biological Sciences, University of Portsmouth, Portsmouth PO1 2DY, United Kingdom

Key Laboratory of Conservation and Utilization of Fish Resources, College of Life Sciences, Neijiang Normal University

Universidad del Magdalena, Grupo de Investigación en Biodiversidad y Ecología Aplicada, Santa Marta AA

Department of Life Science, College of Natural Sciences, Hanyang University, Seoul 04763, Republic of Korea

Fourth Institute of Oceanography, Ministry of Natural Resources

Address：Editorial Office of JOL, 88 Haijun Rd. Qingdao, 266000, China
Tel：+86-532-82898754 Email：jol@qdio.ac.cn
Technical support is provided by Beijing Founder electronics co., LTD 京ICP备09064830号-19 京公网安备11010802024621
It is recommended to read the content of this site in Chrome&IE9+. Please switch to extreme mode in browser 360.
Cookies We use cookies to help provide and enhance our service and tailor content. By continuing, you agree to the use of cookies.

⁰

Tbx4 and Tbx5 gene expression associated with appendage development and its relationship with the absence of the pelvic fin in Pampus argenteus

DOI：10.1007/s00343-023-3011-y

摘要

Abstract

关键词

Keywords

references