Presence of a long nuclear-localization signal sequence in homeodomain transcription factor Nkx1.2

Xinyi LI; Lihui CHEN; Xinyuan WANG; Chen SUN; Guangdong JI; Guobin HU; Zhenhui LIU

doi:10.1007/s00343-023-3007-7

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Presence of a long nuclear-localization signal sequence in homeodomain transcription factor Nkx1.2

Biology | Updated：2024-10-11

- Presence of a long nuclear-localization signal sequence in homeodomain transcription factor Nkx1.2
- Journal of Oceanology and Limnology Vol. 42, Issue 2, Pages: 620-626(2024)
- Affiliations：
  
  1.College of Marine Life Science and Institute of Evolution & Marine Biodiversity, Ocean University of China, Qingdao 266003, China
  2.Laoshan Laboratory, Qingdao 266237, China
- Author bio：
  
  zhenhuiliu@ouc.edu.cn
- Funds：
- DOI：10.1007/s00343-023-3007-7
  CLC：
- Received：09 January 2023，
  
  Online First：03 May 2023，
  
  Published：01 March 2024
- Accepted：
Scan QR Code
LI Xinyi,CHEN Lihui,WANG Xinyuan,et al.Presence of a long nuclear-localization signal sequence in homeodomain transcription factor Nkx1.2[J].Journal of Oceanology and Limnology,2024,42(02):620-626.
DOI：

LI Xinyi,CHEN Lihui,WANG Xinyuan,et al.Presence of a long nuclear-localization signal sequence in homeodomain transcription factor Nkx1.2[J].Journal of Oceanology and Limnology,2024,42(02):620-626. DOI： 10.1007/s00343-023-3007-7.

摘要

Abstract

Homeodomains

a 60-amino acid sequence encoded by 180 nucleotides

are highly conserved DNA-binding motifs that are present in a variety of transcription factors in species ranging from yeast to humans. The NKX proteins belong to the homeodomain (HD)-containing transcription factor family. They play vital roles in the regulation of morphogenesis. NKX1-2 is one member of the NKX subfamily. At present

information about its nuclear localization signal (NLS) sequence is limited. We studied the NLS sequence of zebrafish Nkx1.2 by introducing sequence changes such as deletion

mutation

and truncation

and identified an NLS motif (QNRRTKWKKQ) that is localized at the C-terminus of the homeodomain. Moreover

the deletion of two amino acid residues (RR) in this NLS motif prevents Nkx1.2 from entering the nucleus

indicating that the two amino acids are essential for Nkx1.2 nuclear localization. However

the NLS motif alone is unable to target cytoplasmic protein glutathione S-transferase (GST) to the nucleus. An intact homeodomain is necessary for mediating the complete nuclear transport of cytoplasmic protein. Unlike most nuclear import proteins with short NLS sequences

a long NLS is present in zebrafish Nkx1.2. We also demonstrated that the sequences of homeodomain of NKX1.2 are well conserved among different species. This study is informative to verify the function of the NKX1.2 protein.

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references

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