Effect of dietary supplement of inactivated <italic style="font-style: italic">Lactobacillus</italic> <italic style="font-style: italic">plantarum</italic> Ep-M17 on growth performance, immune response, disease resistance, and intestinal microbiota in <italic style="font-style: italic">Penaeus</italic> <italic style="font-style: italic">vannamei</italic>

Ting WU; Xiaoman HU; Wenlong XU; Yang DU; Jiong CHEN

doi:10.1007/s00343-023-2399-8

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Effect of dietary supplement of inactivated Lactobacillus plantarum Ep-M17 on growth performance, immune response, disease resistance, and intestinal microbiota in Penaeus vannamei

Aquaculture and Fisheries | Updated：2024-10-11

- Effect of dietary supplement of inactivated Lactobacillus plantarum Ep-M17 on growth performance, immune response, disease resistance, and intestinal microbiota in Penaeus vannamei
- Journal of Oceanology and Limnology Vol. 42, Issue 2, Pages: 676-694(2024)
- Affiliations：
  
  1.State Key Laboratory for Managing Biotic and Chemical Threats to the Quality and Safety of Agro-products, Ningbo University, Ningbo 315211, China
  2.Laboratory of Biochemistry and Molecular Biology, School of Marine Sciences, Ningbo University, Ningbo 315211, China
  3.Key Laboratory of Applied Marine Biotechnology of Ministry of Education, Ningbo University, Ningbo 315211, China
- Author bio：
  
  duyang@nbu.edu.cn
  chenjiong@nbu.edu.cn
- Funds：
- DOI：10.1007/s00343-023-2399-8
  CLC：
- Received：04 January 2023，
  
  Published：01 March 2024
- Accepted：
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WU Ting,HU Xiaoman,XU Wenlong,et al.Effect of dietary supplement of inactivated Lactobacillusplantarum Ep-M17 on growth performance, immune response, disease resistance, and intestinal microbiota in Penaeusvannamei[J].Journal of Oceanology and Limnology,2024,42(02):676-694.
DOI：

WU Ting,HU Xiaoman,XU Wenlong,et al.Effect of dietary supplement of inactivated Lactobacillusplantarum Ep-M17 on growth performance, immune response, disease resistance, and intestinal microbiota in Penaeusvannamei[J].Journal of Oceanology and Limnology,2024,42(02):676-694. DOI： 10.1007/s00343-023-2399-8.

摘要

Abstract

Our previous study found that feeding with

Lactobacillus

plantarum

Ep-M17 could effectively affect the growth performance

immune response

and gut microbiota of

Penaeus

vannamei

. However

high temperature and pressure during feed pelletizing is the main problem that can lead to a decrease in the activity of probiotics or cause their inactivation. Further investigation needs to investigate whether inactivated Ep-M17 can exert similar effects as live Ep-M17. Therefore

we evaluated the effects of inactivated

plantarum

Ep-M17 on growth performance

immune response

disease resistance

and gut microbiota in

vannamei

. Results show that adding inactivated Ep-M17 to the feed also promoted body weight gain and increased relative immune protection in shrimp. Also

histological examination revealed that the administration of inactivated Ep-M17 led to improvements in the density and distribution of microvilli in the intestines and enhancements in the abunda

nce of B and R cells in the hepatopancreas. Additionally

the inactivated Ep-M17 supplementation resulted in increased activity levels of nutrient immune-related enzymes in both the shrimp hepatopancreas and intestines. Moreover

it stimulated the expression of

Lvlec

PEN

Crustin

LGBP

Lysozyme

and

proPo

genes in both the hepatopancreas and intestines. Furthermore

the inactivated Ep-M17 also increased bacterial diversity in the gut of shrimp and promoted the abundance of specific flora

facilitating the host organism’s metabolism and immunity to improve the disease resistance of shrimp. Therefore

supplementation of inactivated

plantarum

Ep-M17 in shrimp diets can exert similar effects as live

plantarum

Ep-M17 effectively improving growth performance

gut microbiota

immune response

and disease resistance in

vannamei

关键词

Keywords

references

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Effect of dietary supplement of inactivated Lactobacillus plantarum Ep-M17 on growth performance, immune response, disease resistance, and intestinal microbiota in Penaeus vannamei

DOI：10.1007/s00343-023-2399-8

摘要

Abstract

关键词

Keywords

references