Effects of pyraclostrobin on growth, oxidative stress, and gene expression in relation to stress and ATP-binding cassette transporters in <italic style="font-style: italic">Tetrahymena</italic> <italic style="font-style: italic">thermophila</italic>

Yang LIU; Jiale ZHANG; Peng XIAO; Xin LIU; Yisifu MA; Jing ZHANG; Bangjun ZHANG

doi:10.1007/s00343-023-2378-0

Your Location：

Home >

Browse articles >

Effects of pyraclostrobin on growth, oxidative stress, and gene expression in relation to stress and ATP-binding cassette transporters in Tetrahymena thermophila

Ecology | Updated：2024-10-15

- Effects of pyraclostrobin on growth, oxidative stress, and gene expression in relation to stress and ATP-binding cassette transporters in Tetrahymena thermophila
- Journal of Oceanology and Limnology Vol. 42, Issue 1, Pages: 150-162(2024)
- Affiliations：
  
  1.Henan International Joint Laboratory of Aquatic Ecotoxicology and Health Protection, Xinxiang 453007, China
  2.College of Life Sciences, Henan Normal University, Xinxiang 453007, China
  3.Journal of Henan Normal University, Xinxiang 453007, China
  4.College of Life and Environmental Science, Wenzhou University, Wenzhou 325035, China
  5.Department of Ecosystem Studies, School of Environmental Science, University of Shiga Prefecture, Shiga 522-8533, Japan
- Author bio：
  
  zjl@htu.cn
  zhangbangjun@htu.cn
- Funds：
- DOI：10.1007/s00343-023-2378-0
  CLC：
- Received：28 November 2022，
  
  Accepted：31 January 2023，
  
  Online First：28 February 2023，
  
  Published：03 January 2024
- Accepted：
Scan QR Code
LIU Yang,ZHANG Jiale,XIAO Peng,et al.Research PaperEffects of pyraclostrobin on growth, oxidative stress, and gene expression in relation to stress and ATP-binding cassette transporters in Tetrahymenathermophila[J].Journal of Oceanology and Limnology,2024,42(01):150-162.
DOI：

LIU Yang,ZHANG Jiale,XIAO Peng,et al.Research PaperEffects of pyraclostrobin on growth, oxidative stress, and gene expression in relation to stress and ATP-binding cassette transporters in Tetrahymenathermophila[J].Journal of Oceanology and Limnology,2024,42(01):150-162. DOI： 10.1007/s00343-023-2378-0.

摘要

Abstract

Pyraclostrobin (PYR)

a widely used fungicide

has negative effects on fish and algae

but its toxicity in protozoa remains unclear. In this study

the effects of PYR on the growth

oxidative stress

and gene expression related to stress and ATP-binding cassette (ABC) transporters in

Tetrahymena

thermophila

were investigated. The result showed that the 96-h IC

of PYR against

thermophila

was 17.2 mg/L. Moreover

PYR inhibited the growth of

thermophila

in concentration- or time-dependent manner. A morphological study revealed that the shape and size of

thermophila

changed

and damage of cell membrane surface was observed by scanning electron microscopy after 96 h of PYR exposure. The activities of superoxide dismutase (SOD) and catalase (CAT) increased throughout the experiment. In contrast

the glutathione (GSH) content was increased at 24 h and 48 h of exposure and decreased at 96 h. Moreover

a significant increase in malondialdehyde (MDA) level was observed in

thermophila

after 96 h of exposure. Furthermore

PYR upregulated the

HSP703

HSP705

GPx2

and

ABAC15

gene expression in the 0.1–5-mg/L groups and downregulated the

HSP704

HSP90

TGR

and

ABCC52

mRNA levels at 96 h of exposure. These results suggest that PYR may exert adverse effects on

thermophila

by inducing oxidative stress and changing the gene expression related to ABC transporters and stress

which may enrich the understanding of the toxicity mechanism of PYR in aquatic organisms and provide reference data for aquatic ecological risk assessments.

关键词

Keywords

references

Bard S M . 2000 . Multixenobiotic resistance as a cellular defense mechanism in aquatic organisms . Aquatic Toxicology , 48 ( 4 ): 357 - 389 , https://doi.org/10.1016/S0166-445X(00)00088-6 https://doi.org/10.1016/S0166-445X(00)00088-6 .

Bleier L , Wittig I , Heide H et al . 2015 . Generator-specific targets of mitochondrial reactive oxygen species . Free Radical Biology and Medicine , 78 : 1 - 10 , https://doi.org/10.1016/j.freeradbiomed.2014.10.511 https://doi.org/10.1016/j.freeradbiomed.2014.10.511 .

Blokhina O , Virolainen E , Fagerstedt K V . 2003 . Antioxidants, oxidative damage and oxygen deprivation stress: a review . Annals of Botany , 91 ( 2 ): 179 - 194 , https://doi.org/10.1093/aob/mcf118 https://doi.org/10.1093/aob/mcf118 .

Cai L , Qin X J , Xu Z H et al . 2019 . Comparison of cytotoxicity evaluation of anticancer drugs between real-time cell analysis and CCK-8 method . ACS Omega , 4 ( 7 ): 12036 - 12042 , https://doi.org/10.1021/acsomega.9b01142 https://doi.org/10.1021/acsomega.9b01142 .

Carrasco-Pujante J , Bringas C , Malaina I et al . 2021 . Associative conditioning is a robust systemic behavior in unicellular organisms: an interspecies comparison . Frontiers in Microbiology , 12 : 707086 , https://doi.org/10.3389/fmicb.2021.707086 https://doi.org/10.3389/fmicb.2021.707086 .

Chen L G , Tang L Z , Bai Y C . 2022 . Combination effect of perfluorobutanesulfonate and probiotics on thyroid endocrine system of zebrafish . Journal of Henan Normal University (Natural Science Edition) , 50 ( 1 ): 1 - 11 , https://doi.org/10.16366/j.cnki.1000-2367.2022.01.001 https://doi.org/10.16366/j.cnki.1000-2367.2022.01.001 .

Choi J , Liu R M , Forman H J . 1997 . Adaptation to oxidative stress: quinone-mediated protection of signaling in rat lung epithelial L2 cells . Biochemical Pharmacology , 53 ( 7 ): 987 - 993 , https://doi.org/10.1016/S0006-2952(96)00867-2 https://doi.org/10.1016/S0006-2952(96)00867-2 .

Couto N , Malys N , Gaskell S J et al . 2013 . Partition and turnover of glutathione reductase from Saccharomyces cerevisiae : a proteomic approach . Journal of Proteome Research , 12 ( 6 ): 2885 - 2894 , https://doi.org/10.1021/pr4001948 https://doi.org/10.1021/pr4001948 .

Cui F , Chai T T , Liu X X et al . 2017 . Toxicity of three strobilurins (kresoxim-methyl, pyraclostrobin, and trifloxystrobin) on Daphnia magna . Environmental Toxicology and Chemistry , 36 ( 1 ): 182 - 189 , https://doi.org/10.1002/etc.3520 https://doi.org/10.1002/etc.3520 .

Dean M , Hamon Y , Chimini G . 2001 . The human ATP-binding cassette (ABC) transporter superfamily . Journal of Lipid Research , 42 ( 7 ): 1007 - 1017 , https://doi.org/10.1016/S0022-2275(20)31588-1 https://doi.org/10.1016/S0022-2275(20)31588-1 .

Díaz S , Martín-González A , Cubas L et al . 2016 . High resistance of Tetrahymena thermophila to paraquat: mitochondrial alterations, oxidative stress and antioxidant genes expression . Chemosphere , 144 : 909 - 917 , https://doi.org/10.1016/j.chemosphere.2015.09.010 https://doi.org/10.1016/j.chemosphere.2015.09.010 .

Ding W K , Shangguan Y Y , Zhu Y Q et al . 2021 . Negative impacts of microcystin-LR and glyphosate on zebrafish intestine: linked with gut microbiota and microRNAs? Environmental Pollution , 286 : 117685 , https://doi.org/10.1016/j.envpol.2021.117685 https://doi.org/10.1016/j.envpol.2021.117685 .

Eisen J A , Coyne R S , Wu M et al . 2006 . Macronuclear genome sequence of the ciliate Tetrahymena thermophila , a model eukaryote . PLoS Biology , 4 ( 9 ): e286 , https://doi.org/10.1371/journal.pbio.0040286 https://doi.org/10.1371/journal.pbio.0040286 .

Fan R F , Wang C C . 2022 . Research progress of bisphenol A-induced neuronal apoptosis and inflammatory death . Journal of Henan Normal University (Natrual Science Edition) , 50 ( 1 ): 12 - 22 , https://doi.org/10.16366/j.cnki.1000-2367.2022.01.002 https://doi.org/10.16366/j.cnki.1000-2367.2022.01.002 .

Feder M E , Hofmann G E . 1999 . Heat-shock proteins, molecular chaperones, and the stress response: evolutionary and ecological physiology . Annual Review of Physiology , 61 ( 1 ): 243 - 282 , https://doi.org/10.1146/annurev.physiol.61.1.243 https://doi.org/10.1146/annurev.physiol.61.1.243 .

Fidder B N , Reátegui-Zirena E G , Olson A D et al . 2016 . Energetic endpoints provide early indicators of life history effects in a freshwater gastropod exposed to the fungicide, pyraclostrobin . Environmental Pollution , 211 : 183 - 190 , https://doi.org/10.1016/j.envpol.2015.12.018 https://doi.org/10.1016/j.envpol.2015.12.018 .

Fridovich I . 2004 . Mitochondria: are they the seat of senescence? Aging Cell , 3 ( 1 ): 13 - 16 , https://doi.org/10.1046/j.1474-9728.2003.00075.x https://doi.org/10.1046/j.1474-9728.2003.00075.x .

Gao L , Yuan T , Cheng P et al . 2015 . Effects of triclosan and triclocarban on the growth inhibition, cell viability, genotoxicity and multixenobiotic resistance responses of Tetrahymena thermophila . Chemosphere , 139 : 434 - 440 , https://doi.org/10.1016/j.chemosphere.2015.07.059 https://doi.org/10.1016/j.chemosphere.2015.07.059 .

Guha P , Dey A , Sen R et al . 2011 . Intracellular GSH depletion triggered mitochondrial Bax translocation to accomplish resveratrol-induced apoptosis in the U937 cell line . Journal of Pharmacology and Experimental Therapeutics , 336 ( 1 ): 206 - 214 , https://doi.org/10.1124/jpet.110.171983 https://doi.org/10.1124/jpet.110.171983 .

Guo J , Pu Y , Zhong L Q et al . 2021 . Lead impaired immune function and tissue integrity in yellow catfish ( Peltobargus fulvidraco ) by mediating oxidative stress, inflammatory response and apoptosis . Ecotoxicology and Environmental Safety , 226 : 112857 , https://doi.org/10.1016/j.ecoenv.2021.112857 https://doi.org/10.1016/j.ecoenv.2021.112857 .

Guo X Y , Wu W Z , Song N H et al . 2017 . Residue dynamics and risk assessment of pyraclostrobin in rice, plants, hulls, field soil, and paddy water . Human and Ecological Risk Assessment , 23 ( 1 ): 67 - 81 , https://doi.org/10.1080/10807039.2016.1222579 https://doi.org/10.1080/10807039.2016.1222579 .

Hooser E A , Belden J B , Smith L M et al . 2012 . Acute toxicity of three strobilurin fungicide formulations and their active ingredients to tadpoles . Ecotoxicology , 21 ( 5 ): 1458 - 1464 , https://doi.org/10.1007/s10646-012-0899-y https://doi.org/10.1007/s10646-012-0899-y .

Huang A G , Tu X , Liu L et al . 2016 . The oxidative stress response of myclobutanil and cyproconazole on Tetrahymena thermophila . Environmental Toxicology and Pharmacology , 41 : 211 - 218 , https://doi.org/10.1016/j.etap.2015.12.008 https://doi.org/10.1016/j.etap.2015.12.008 .

Huang X P , Yang S , Li B X et al . 2021 . Comparative toxicity of multiple exposure routes of pyracl ostrobin in adult zebrafish ( Danio rerio ) . Science of the Total Environment , 777 : 145957 , https://doi.org/10.1016/j.scitotenv.2021.145957 https://doi.org/10.1016/j.scitotenv.2021.145957 .

Ikwegbue P C , Masamba P , Oyinloye B E et al . 2017 . Roles of heat shock proteins in apoptosis, oxidative stress, human inflammatory diseases, and cancer . Pharmaceuticals , 11 ( 1 ): 2 , https://doi.org/10.3390/ph11010002 https://doi.org/10.3390/ph11010002 .

Juganson K , Mortimer M , Ivask A et al . 2017 . Mechanisms of toxic action of silver nanoparticles in the protozoan Tetrahymena thermophila : from gene expression to phenotypic events . Environmental Pollution , 225 : 481 - 489 , https://doi.org/10.1016/j.envpol.2017.03.013 https://doi.org/10.1016/j.envpol.2017.03.013 .

Kalmar B , Greensmith L . 2009 . Induction of heat shock proteins for protection against oxidative stress . Advanced Drug Delivery Reviews , 61 ( 4 ): 310 - 318 , https://doi.org/10.1016/j.addr.2009.02.003 https://doi.org/10.1016/j.addr.2009.02.003 .

Kim C , Choe H , Park J et al . 2021 . Molecular mechanisms of developmental toxicities of azoxystrobin and pyraclostrobin toward zebrafish ( Danio rerio ) embryos: visualization of abnormal development using two transgenic lines . Environmental Pollution , 270 : 116087 , https://doi.org/10.1016/j.envpol.2020.116087 https://doi.org/10.1016/j.envpol.2020.116087 .

Lainhart W , Stolfa G , Koudelka G B . 2009 . Shiga toxin as a bacterial defense against a eukaryotic predator, Tetrahymena thermophila . Journal of Bacteriology , 191 ( 16 ): 5116 - 5122 , https://doi.org/10.1128/JB.00508-09 https://doi.org/10.1128/JB.00508-09 .

Li D , Liu M Y , Yang Y S et al . 2016 . Strong lethality and teratogenicity of strobilurins on Xenopus tropicalis embryos: basing on ten agricultural fungicides . Environmental Pollution , 208 : 868 - 874 , https://doi.org/10.1016/j.envpol.2015.11.010 https://doi.org/10.1016/j.envpol.2015.11.010 .

Li H , Cao F J , Zhao F et al . 2018 . Developmental toxicity, oxidative stress and immunotoxicity induced by three strobilurins (pyraclostrobin, trifloxystrobin and picoxystrobin) in zebrafish embryos . Chemosphere , 207 : 781 - 790 , https://doi.‍org/10.1016/j.chemosphere.‍2018.05.146 https://doi.‍org/10.1016/j.chemosphere.‍2018.05.146 . https://do 10.1016/j.chemosphere.2018.05.146 http://dx.doi.org/10.1016/j.chemosphere.2018.05.146

Li H , Jing T F , Li T B et al . 2021a . Ecotoxicological effects of pyraclostrobin on tilapia ( Oreochromis niloticus ) via various exposure routes . Environmental Pollution , 285 : 117188 , https://doi.org/10.1016/j.envpol.2021.117188 https://doi.org/10.1016/j.envpol.2021.117188 .

Li H , Zhao F , Cao F J et al . 2019 . Mitochondrial dysfunction-based cardiotoxicity and neurotoxicity induced by pyraclostrobin in zebrafish larvae . Environmental Pollution , 251 : 203 - 211 , https://doi.org/10.1016/j.envpol.2019.04.122 https://doi.org/10.1016/j.envpol.2019.04.122 .

Li M H . 2019 . Comparative toxicities of 10 widely used biocides in three freshwater invertebrate species . Chemistry and Ecology , 35 ( 5 ): 472 - 482 , https://doi.org/10.1080/02757540.2019.1579311 https://doi.org/10.1080/02757540.2019.1579311 .

Li X Y , Qin Y J , Wang Y et al . 2021b . Relative comparison of strobilurin fungicides at environmental levels: focus on mitochondrial function and larval activity in early staged zebrafish ( Danio rerio ) . Toxicology , 452 : 152706 , https://doi.org/10.1016/j.tox.2021.152706 https://doi.org/10.1016/j.tox.2021.152706 .

Liu L , Jiang C , Wu Z Q et al . 2013 . Toxic effects of three strobilurins (trifloxystrobin, azoxystrobin and kresoxim-methyl) on mRNA expression and antioxidant enzymes in grass carp ( Ctenopharyngodon idella ) juveniles . Ecotoxicology and Environmental Safety , 98 : 297 - 302 , https://doi.org/10.1016/j.ecoenv.2013.10.011 https://doi.org/10.1016/j.ecoenv.2013.10.011 .

Liu X X , Wang Y , Chen H et al . 2018 . Acute toxicity and associated mechanisms of four strobilurins in algae . Environmental Toxicology and Pharmacology , 60 : 12 - 16 , https://doi.org/10.1016/j.etap.2018.03.021 https://doi.org/10.1016/j.etap.2018.03.021 .

Liu Y , Zhang J L , Zhao H Y et al . 2022 . Effects of polyvinyl chloride microplastics on reproduction, oxidative stress and reproduction and detoxification-related genes in Daphnia magna . Comparative Biochemistry and Physiology Part C : Toxicology & Pharmacology , 254 : 109269 , https://doi.org/10.1016/j.cbpc.2022.109269 https://doi.org/10.1016/j.cbpc.2022.109269 .

Livak K J , Schmittgen T D . 2001 . Analysis of relative gene expression data using real-time quantitative PCR and the 2 -ΔΔ C t method . Methods , 25 ( 4 ): 402 - 408 , https://doi.org/10.1006/meth.2001.1262 https://doi.org/10.1006/meth.2001.1262 .

Luz A L , Kassotis C D , Stapleton H M et al . 2018 . The high-production volume fungicide pyraclostrobin induces triglyceride accumulation associated with mitochondrial dysfunction, and promotes adipocyte differentiation independent of PPARγ activation, in 3T3-L1 cells . Toxicology , 393 : 150 - 159 , https://doi.org/10.1016/j.tox.2017.11.010 https://doi.org/10.1016/j.tox.2017.11.010 .

Mao L G , Jia W , Zhang L et al . 2020 . Embryonic development and oxidative stress effects in the larvae and adult fish livers of zebrafish ( Danio rerio ) exposed to the strobilurin fungicides, kresoxim-methyl and pyraclostrobin . Science of the Total Environment , 729 : 139031 , https://doi.org/10.1016/j.scitotenv.2020.139031 https://doi.org/10.1016/j.scitotenv.2020.139031 .

Mastrantonio V , Ferrari M , Epis S et al . 2017 . Gene expression modulation of ABC transporter genes in response to permethrin in adults of the mosquito malaria vector Anopheles stephensi . Acta Tropica , 171 : 37 - 43 , https://doi.org/10.1016/j.actatropica.2017.03.012 https://doi.org/10.1016/j.actatropica.2017.03.012 .

Maurya R , Dubey K , Singh D et al . 2019 . Effect of difenoconazole fungicide on physiological responses and ultrastructural modifications in model organism Tetrahymena pyriformis . Ecotoxicology and Environmental Safety , 182 : 109375 , https://doi.org/10.1016/j.ecoenv.2019.109375 https://doi.org/10.1016/j.ecoenv.2019.109375 .

Maurya R , Pandey A K . 2020 . Importance of p rotozoa Tetrahymena in toxicological studies: a review . Science of the Total Environment , 741 : 140058 , https://doi.org/10.1016/j.scitotenv.2020.140058 https://doi.org/10.1016/j.scitotenv.2020.140058 .

Mimbs W H IV , Cusaac J P W , Smith L M et al . 2016 . Occurrence of current-use fungicides and bifenthrin in Rainwater Basin wetlands . Chemosphere , 159 : 275 - 281 , https://doi.org/10.1016/j.chemosphere.2016.06.012 https://doi.org/10.1016/j.chemosphere.2016.06.012 .

Morrison S A , Belden J B . 2016 . Development of Helisoma trivolvis pond snails as biological samplers for biomonitoring of current-use pesticides . Environmental Toxicology and Chemistry , 35 ( 9 ): 2320 - 2329 , https://doi.org/10.1002/etc.3400 https://doi.org/10.1002/etc.3400 .

Morrison S A , Mcmurry S T , Smith L M et al . 2013 . Acute toxicity of pyraclostrobin and trifloxystrobin to Hyalella azteca . Environmental Toxicology and Chemistry , 32 ( 7 ): 1516 - 1525 , https://doi.org/10.1002/etc.2228 https://doi.org/10.1002/etc.2228 .

Ning Y Z , Dang H , Liu G L et al . 2015 . ATP-binding cassette transporter enhances tolerance to DDT in Tetrahymena . Science China Life Sciences , 58 ( 3 ): 297 - 304 , https://doi.org/10.1007/s11427-014-4743-x https://doi.org/10.1007/s11427-014-4743-x .

Ochoa-Acuña H G , Bialkowski W , Yale G et al . 2009 . Toxicity of soybean rust fungicides to freshwater algae and Daphnia magna . Ecotoxicology , 18 ( 4 ): 440 - 446 , https://doi.org/10.1007/s10646-009-0298-1 https://doi.org/10.1007/s10646-009-0298-1 .

Ott M , Gogvadze V , Orrenius S et al . 2007 . Mitochondria, oxidative stress and cell death . Apoptosis , 12 ( 5 ): 913 - 922 , https://doi.org/10.1007/s10495-007-0756-2 https://doi.org/10.1007/s10495-007-0756-2 .

Pan Y Y , Tian L N , Zhao Q L et al . 2022 . Evaluation of the acute toxic effects of crude oil on intertidal mudskipper ( Boleophthalmus pectinirostris ) based on antioxidant enzyme activity and the integrated biomarker response . Environmental Pollution , 292 : 118341 , https://doi.org/10.1016/j.envpol.2021.118341 https://doi.org/10.1016/j.envpol.2021.118341 .

Pisoschi A M , Pop A . 2015 . The role of antioxidants in the chemistry of oxidative stress: a review . European Journal of Medicinal Chemistry , 97 : 55 - 74 , https://doi.org/10.1016/j.ejmech.2015.04.040 https://doi.org/10.1016/j.ejmech.2015.04.040 .

Rahal A , Kumar A , Singh V et al . 2014 . Oxidative stress, prooxidants, and antioxidants: the interplay . BioMed Research International , 2014 : 761264 , https://doi.org/10.1155/2014/761264 https://doi.org/10.1155/2014/761264 .

Shi M M , Kugelman A , Iwamoto T et al . 1994 . Quinone-induced oxidative stress elevates glutathione and induces γ-glutamylcysteine synthetase activity in rat lung epithelial L2 cells . Journal of Biological Chemistry , 269 ( 42 ): 26512 - 26517 , https://doi.org/10.1016/S0021-9258(18)47224-9 https://doi.org/10.1016/S0021-9258(18)47224-9 .

Su L J , Zhang J H , Gomez H et al . 2019 . Reactive oxygen species-induced lipid peroxidation in apoptosis, autophagy, and ferroptosis . Oxidative Medicine and Cellular Longevity , 2019 : 5080843 , https://doi.org/10.1155/2019/5080843 https://doi.org/10.1155/2019/5080843 .

Tahedl H , Häder D P . 2001 . Automated biomonitoring using real time movement analysis of Euglena gracilis . Ecotoxicology and Environmental Safety , 48 ( 2 ): 161 - 169 , https://doi.org/10.1006/eesa.2000.2004 https://doi.org/10.1006/eesa.2000.2004 .

Veedu S K , Ayyasamy G , Tamilselvan H et al . 2022 . Single and joint toxicity assessment of acetamiprid and thiamethoxam neonicotinoids pesticides on biochemical indices and antioxidant enzyme activities of a freshwater fish Catla catla . Comparative Biochemistry and Physiology Part C : Toxicology & Pharmacology , 257 : 109336 , https://doi.org/10.1016/j.cbpc.2022.109336 https://doi.org/10.1016/j.cbpc.2022.109336 .

Wang K , Sun Z H , Yang L D et al . 2020 . Respiratory toxicity of azoxystrobin, pyraclostrobin and coumoxystrobin on Chlorella vulgaris . Bulletin of Environmental Contamination and Toxicology , 104 ( 6 ): 799 - 803 , https://doi.org/10.1007/s00128-020-02869-y https://doi.org/10.1007/s00128-020-02869-y .

Wang X H , Li X Y , Wang Y et al . 2021 . A comprehensive review of strobilurin fungicide toxicity in aquatic species: emphasis on mode of action from the zebrafish model . Environmental Pollution , 275 : 116671 , https://doi.org/10.1016/j.envpol.2021.116671 https://doi.org/10.1016/j.envpol.2021.116671 .

Wightwick A M , Bui A D , Zhang P et al . 2012 . Environmental fate of fungicides in surface waters of a horticultural-production catchment in southeastern Australia . Archives of Environmental Contamination and Toxicology , 62 ( 3 ): 380 - 390 , https://doi.org/10.1007/s00244-011-9710-y https://doi.org/10.1007/s00244-011-9710-y .

Williams D L , Bonilla M , Gladyshev V N et al . 2013 . Thioredoxin glutathione reductase-dependent redox networks in platyhelminth parasites . Antioxidants & Redox Signaling , 19 ( 7 ): 735 - 745 , https://doi.org/10.1089/ars.2012.4670 https://doi.org/10.1089/ars.2012.4670 .

Wu C , Guo W B , Liu Y Y et al . 2021 . Perturbation of calcium homeostasis and multixenobiotic resistance by nanoplastics in the ciliate Tetrahymena thermophila . Journal of Hazardous Materials , 403 : 123923 , https://doi.org/10.1016/j.jhazmat.2020.123923 https://doi.org/10.1016/j.jhazmat.2020.123923 .

Xiong J , Feng L F , Yuan D X et al . 2010 . Genome-wide identification and evolution of ATP-binding cassette transporters in the ciliate Tetrahymena thermophila : a case of functional divergence in a multigene family . BMC Evolutionary Biology , 10 ( 1 ): 330 , https://doi.org/10.1186/1471-2148-10-330 https://doi.org/10.1186/1471-2148-10-330 .

Yang Y . 2014 . The overview of 2013 China pesticide industry and the prediction and analysis of industrial development in 2014 . Agrochemicals , 53 ( 2 ): 145 - 148 , https://doi.org/10.16820/j.cnki.1006-0413.2014.02.022 https://doi.org/10.16820/j.cnki.1006-0413.2014.02.022 .

Yu M , Wang S H , Luo Y R et al . 2009 . Effects of the 1-alkyl-3-methylimidazolium bromide ionic liquids on the antioxidant defense system of Daphnia magna . Ecotoxicology and Environmental Safety , 72 ( 6 ): 1798 - 1804 , https://doi.org/10.1016/j.ecoenv.2009.05.002 https://doi.org/10.1016/j.ecoenv.2009.05.002 .

Yu T , Barchetta S , Pucciarelli S et al . 2012 . A novel robust heat-inducible promoter for heterologous gene expression in Tetrahymena thermophila . Protist , 163 ( 2 ): 284 - 295 , https://doi.org/10.1016/j.protis.2011.07.003 https://doi.org/10.1016/j.protis.2011.07.003 .

Zhang B J , Zhang J L , Chen L G . 2021 . Effects of 2,4-D on the antioxidant system and the gene expression of the earthworm Eisenia fetida . Journal of Henan Normal University (Natural Science Edition) , 49 ( 5 ): 89 - 95 , https://doi.org/10.16366/j.cnki.1000-2367.2021.05.012 https://doi.org/10.16366/j.cnki.1000-2367.2021.05.012 .

Zhang C , Wang J , Zhang S et al . 2017 . Acute and subchronic toxicity of pyraclostrobin in zebrafish ( Danio rerio ) . Chemosphere , 188 : 510 - 516 , https://doi.org/10.1016/j.chemosphere.2017.09.025 https://doi.org/10.1016/j.chemosphere.2017.09.025 .

Zhang C , Zhou T T , Xu Y Q et al . 2020 . Ecotoxicology of strobilurin fungicides . Science of the Total Environment , 742 : 140611 , https://doi.org/10.1016/j.scitotenv.2020.140611 https://doi.org/10.1016/j.scitotenv.2020.140611 .

Zhao H Y , Zhang J L , Rajeshkumar S et al . 2022 . Hepatopancreas toxicity and immunotoxicity of a fungicide, pyraclostrobin, on common carp . Comparative Biochemistry and Physiology Part C : Toxicology & Pharmacology , 262 : 109445 , https://doi.org/10.1016/j.cbpc.2022.109445 https://doi.org/10.1016/j.cbpc.2022.109445 .

Zhao Y , Fan J H , Luo Y et al . 2019 . Di-(2-ethylhexyl) phthalate (DEHP)-induced hepatotoxicity in quail ( Coturnix japonica ) via suppression of the heat shock response . Chemosphere , 228 : 685 - 693 , https://doi.org/10.1016/j.chemosphere.2019.04.172 https://doi.org/10.1016/j.chemosphere.2019.04.172 .

Zhu B , Liu G L , Liu L et al . 2015 . Assessment of trifloxystrobin uptake kinetics, developmental toxicity and mRNA expression in rare minnow embryos . Chemo sphere , 120 : 447 - 455 , https://doi.org/10.1016/j.chemosphere.‍2014.07.100 https://doi.org/10.1016/j.chemosphere.‍2014.07.100 .

Views

Downloads

CSCD

Alert me when the article has been cited

Submit

Tools

Publicity Resources

Marine fungal metabolite butyrolactone I improves Aβ_1-42-induced cognitive impairment in mice via gut-brain axial microbiota remodeling, anti-inflammation, and antioxidation

Dopamine mediates the link between aggressive behavior and biogenic amine receptor genes in mud crab Scylla paramamosain

Effects of sulfate content and salinity on growth, oxygen consumption, Na⁺/K⁺-ATPase activity, and hepatopancreas histopathology of Pacific white shrimp Litopenaeus vannamei

Size-dependent toxicity of microplastics induced gut cell apoptosis in brine shrimp Artemia parthenogenetica through p53 signaling pathway

Multivariable Constrained Model Predictive Control ofAero-Engine Based on Augmented Predictive Model

Related Author

Fuyan YANG

Longjian ZHOU

Jiahang DENG

Yuan WANG

Zhiyou YANG

Yongping ZHANG

Yayue LIU

Yi ZHANG

Related Institution

Guangdong Provincial Key Laboratory of Aquatic Product Processing and Safety, Guangdong Provincial Engineering Laboratory for Marine Biological Products, Marine Biomedicine R&D Center at Shenzhen Institute of Guangdong Ocean University, Zhanjiang Municipal Key Laboratory of Marine Drugs and Nutrition for Brain Health, Research Institute for Marine Drugs and Nutrition, College of Food Science and Technology, Guangdong Ocean University

Southern Marine Science and Engineering Guangdong Laboratory

Collaborative Innovation Center of Seafood Deep Processing, Dalian Polytechnic University

School of Marine Sciences, Ningbo University

Ningbo Institute of Oceanography

Address：Editorial Office of JOL, 88 Haijun Rd. Qingdao, 266000, China
Tel：+86-532-82898754 Email：jol@qdio.ac.cn
Technical support is provided by Beijing Founder electronics co., LTD 京ICP备09064830号-19 京公网安备11010802024621
It is recommended to read the content of this site in Chrome&IE9+. Please switch to extreme mode in browser 360.
Cookies We use cookies to help provide and enhance our service and tailor content. By continuing, you agree to the use of cookies.

⁰

Effects of pyraclostrobin on growth, oxidative stress, and gene expression in relation to stress and ATP-binding cassette transporters in Tetrahymena thermophila

DOI：10.1007/s00343-023-2378-0

摘要

Abstract

关键词

Keywords

references