Transcriptome analysis reveals immune-related genes in tissues of <italic style="font-style: italic">Vibrio anguillarum</italic>-infected turbot <italic style="font-style: italic">Scophthalmus maximus</italic>

Yuting SONG; Maqsood Ahmed SOOMRO; Xianzhi DONG; Guobin HU

doi:10.1007/s00343-023-2363-7

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Transcriptome analysis reveals immune-related genes in tissues of Vibrio anguillarum-infected turbot Scophthalmus maximus

Aquaculture and Fisheries | Updated：2024-10-15

- Transcriptome analysis reveals immune-related genes in tissues of Vibrio anguillarum-infected turbot Scophthalmus maximus
- Journal of Oceanology and Limnology Vol. 42, Issue 1, Pages: 332-344(2024)
- Affiliations：
  
  1.College of Marine Life Sciences, Ocean University of China, Qingdao 266003, China
  2.Institute of Evolution & Marine Biodiversity, Ocean University of China, Qingdao 266003, China
  3.Institute of Biophysis, Chinese Academy of Sciences, Beijing 100101, China
- Author bio：
  
  xzdong@ibp.ac.cn
  huguobin@ouc.edu.cn
- Funds：
- DOI：10.1007/s00343-023-2363-7
  CLC：
- Received：15 September 2022，
  
  Accepted：04 November 2022，
  
  Online First：16 February 2023，
  
  Published：03 January 2024
- Accepted：
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SONG Yuting,SOOMRO Maqsood Ahmed,DONG Xianzhi,et al.Research PaperTranscriptome analysis reveals immune-related genes in tissues of Vibrio anguillarum-infected turbot Scophthalmus maximus[J].Journal of Oceanology and Limnology,2024,42(01):332-344.
DOI：

SONG Yuting,SOOMRO Maqsood Ahmed,DONG Xianzhi,et al.Research PaperTranscriptome analysis reveals immune-related genes in tissues of Vibrio anguillarum-infected turbot Scophthalmus maximus[J].Journal of Oceanology and Limnology,2024,42(01):332-344. DOI： 10.1007/s00343-023-2363-7.

摘要

Abstract

Turbot

Scophthalmus maximus

is an important mariculture fish species with high economic value.

However

the bacterial diseases caused by

Vibrio anguillarum

infection bring huge economic losses to the turbot aquaculture industry. To understand the immune response of the turbot against

anguillarum

infection and to explore novel immune-related genes

the transcriptome analysis of turbot spleen and gills were conducted after

anguillarum

infection. Differentially expressed genes (DEGs) were identified in spleen and gill of the turbot amounted to 17 261 and 16 436

respectively. A large number of immune-related DEGs were enriched in cytokine-cytokine receptor interaction signaling pathway

and the others by the kyoto encyclopedia of genes and genomes (KEGG) enrichment. The gene ontology (GO) classification analysis revealed that

anguillarum

infection had the greatest effect on biological processes and cellular components. Twelve immune-related DEGs were identified in the spleen (

cstl

egfl6

lamb21

v2rx4

calcr

and

gpr78a

) and gills (

ghra

sh3gl2a

cst12

inhbaa

cxcl8

and

) by heat map. The protein-protein interaction (PPI) networks were constructed to analyze the

immune mechanism. The results demonstrate that the maturation and antigen processing of major histocompatibility complex (MHC) class II molecule

and calcitonin- or adrenomedullin-regulated physiological activity were important events in the immunity of turbot against

anguillarum

infection. In the gills

the protein interactions in TGF-β signaling pathway

production of inflammatory factors

and endocytosis regulation were most significant. Our research laid a foundation for discovering novel immune-related genes and enriching the knowledge of immune mechanisms of turbot against

anguillarum

infection.

关键词

Keywords

references

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Transcriptome analysis reveals immune-related genes in tissues of Vibrio anguillarum-infected turbot Scophthalmus maximus

DOI：10.1007/s00343-023-2363-7

摘要

Abstract

关键词

Keywords

references