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Characterization, expression dynamics, and potential function of OPA1 for regulation of mitochondrial morphology during spermiogenesis in
Phascolosoma esculenta - Journal of Oceanology and Limnology Vol. 42, Issue 1, Pages: 187-200(2024)
- Affiliations：
1.Key Laboratory of Applied Marine Biotechnology by the Ministry of Education and Key Laboratory of Marine Biotechnology of Zhejiang Province, School of Marine Sciences, Ningbo University, Ningbo 315211, China
2.College of Ecology, Lishui University, Lishui 323000, China
- Author bio：
tangdaojun@nbu.edu.cn
zhujunquan@nbu.edu.cn
- Funds：
DOI：10.1007/s00343-023-2343-y
CLC：Received：19 October 2022，
Accepted：25 November 2022，
Online First：08 February 2023，
Published：03 January 2024
- Accepted：
Scan QR Code
GAO Xinming,FENG Binbin,DU Chen,et al.Research PaperCharacterization, expression dynamics, and potential function of OPA1 for regulation of mitochondrial morphology during spermiogenesis in ,Phascolosoma,esculenta[J].Journal of Oceanology and Limnology,2024,42(01):187-200.
GAO Xinming,FENG Binbin,DU Chen,et al.Research PaperCharacterization, expression dynamics, and potential function of OPA1 for regulation of mitochondrial morphology during spermiogenesis in ,Phascolosoma,esculenta[J].Journal of Oceanology and Limnology,2024,42(01):187-200. DOI： 10.1007/s00343-023-2343-y.
摘要
Abstract
Mitochondria undergo morphological changes during spermatogenesis in some animals. The mechanism and role of mitochondrial morphology regulation
however
remain somewhat unclear. In this study
we analyzed the molecular characteristics
expression dynamics and subcellular localization of optic atrophy protein 1 (OPA1)
a mitochondrial fusion and cristae maintenance-related protein
to reveal the possible regulatory mechanisms underlying mitochondrial morphology in
Phascolosoma
esculenta
spermiogenesis. The full-length cDNA of the
P
.
esculenta
opa1
gene (
Pe
-
opa1
) is 3 743 bp in length and encodes 975 amino acids. The
Pe
-OPA1 protein is highly conservative and includes a transmembrane domain
a GTPase domain
two helical bundle domains
and a lipid-interacting stalk. Gene and protein expression was higher in the coelomic fluid (a site of spermatid development) of male
P
.
esculenta
and increased first and then decreased from March to December. Moreover
their expression during the breeding stage was significantl
y higher than during the non-breeding stage
suggesting that
Pe
-OPA1 is involved in
P
.
esculenta
reproduction. The
Pe
-OPA1 protein was more abundant in components consisting of many spermatids than in components without
indicating that
Pe
-OPA1 mainly plays a role in the spermatid in coelomic fluid. Moreover
Pe
-OPA1 was mainly detected in the spermatid mitochondria. Immunofluorescence experiments showed that the
Pe
-OPA1 are constitutively expressed and co-localized with mitochondria during spermiogenesis
suggesting its involvement in
P
.
esculenta
spermiogenesis. These results provide evidence for
Pe
-OPA1’s involvement in the regulation of mitochondrial morphology during spermiogenesis.
关键词
Keywords
references
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