Proteomic response of <italic style="font-style: italic">Phaeocystis</italic> <italic style="font-style: italic">globosa</italic> to nitrogen limitation

Haisu LIU; Ruiwang WEI; Qiangyong LEI; Lei CUI; Songhui LÜ

doi:10.1007/s00343-023-2313-4

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Proteomic response of Phaeocystis globosa to nitrogen limitation

Ecology | Updated：2024-10-15

- Proteomic response of Phaeocystis globosa to nitrogen limitation
- Journal of Oceanology and Limnology Vol. 42, Issue 1, Pages: 141-149(2024)
- Affiliations：
  
  1.Key Laboratory of Eutrophication and Red Tide Prevention, Research Center for Harmful Algae and Marine Biology, Jinan University, Guangzhou 510632, China
  2.Southern Marine Science and Engineering Guangdong Laboratory, Zhuhai 519082, China
  3.National Engineering Laboratory of Electronic and Information Standardization, Shenzhen 518057, China
- Author bio：
  
  leicui@jnu.edu.cn
  lusonghui@163.com
- Funds：
- DOI：10.1007/s00343-023-2313-4
  CLC：
- Received：09 November 2022，
  
  Accepted：16 January 2023，
  
  Online First：17 March 2023，
  
  Published：03 January 2024
- Accepted：
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LIU Haisu,WEI Ruiwang,LEI Qiangyong,et al.Research Paper Proteomic response of Phaeocystisglobosa to nitrogen limitation[J].Journal of Oceanology and Limnology,2024,42(01):141-149.
DOI：

LIU Haisu,WEI Ruiwang,LEI Qiangyong,et al.Research Paper Proteomic response of Phaeocystisglobosa to nitrogen limitation[J].Journal of Oceanology and Limnology,2024,42(01):141-149. DOI： 10.1007/s00343-023-2313-4.

摘要

Abstract

Phaeocystis

globosa

is an important unicellular eukaryotic alga that can also form colonies.

globosa

can cause massive harmful algal blooms and plays an important role in the global carbon or sulfur cycling. Thus far

the ecophysiology of

globosa

has been investigated by numerous studies. However

the proteomic response of

globosa

to nitrogen depletion remains largely unknown. We compared four protein preparation methods of

globosa

for two-dimensional electrophoresis (2-DE) (Urea/Triton X-100 with trichloroacetic acid (TCA)/acetone precipitation; TCA/acetone precipitation; Radio Immuno Precipitation Assay (RIPA) with TCA/acetone precipitation; and Tris buffer). Results show that the combination of RIPA with TCA/acetone precipitation had a clear gel background and showed the best protein spot separation effect

based on which the proteomic response to nitrogen depletion was studied using 2-DE. In addition

we identified six differentially expressed proteins whose relative abundance increased or decreased more than 1.5-fold (

0.05). Most proteins could not be identified

which might be attributed to the lack of genomic sequences of

globosa

. Under nitrogen limitation

replication protein-like

RNA ligase

and sn-glycerol-3-phospha

te dehydrogenase were reduced

which may decrease the DNA replication level and ATP production in

globosa

cells. The increase of endonuclease Ⅲ and transcriptional regulator enzyme may affect the metabolic and antioxidant function of

globosa

cells and induce cell apoptosis. These findings provide a basis for further proteomic study of

globosa

and the optimization of protein preparation methods of marine microalgae.

关键词

Keywords

references

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⁰

Proteomic response of Phaeocystis globosa to nitrogen limitation

DOI：10.1007/s00343-023-2313-4

摘要

Abstract

关键词

Keywords

references