Screening for lipophilic marine toxins and their potential producers in coastal waters of Weihai in autumn, 2020

Huixia GENG; Hongxiao SUN; Chao LIU; Fanzhou KONG; Qingchun ZHANG; Tian YAN; Rencheng YU

doi:10.1007/s00343-022-2301-0

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Screening for lipophilic marine toxins and their potential producers in coastal waters of Weihai in autumn, 2020

Marine algal toxins | Updated：2023-05-20

- Screening for lipophilic marine toxins and their potential producers in coastal waters of Weihai in autumn, 2020
- Journal of Oceanology and Limnology Vol. 40, Issue 6, Pages: 2218-2230(2022)
- Affiliations：
  
  1.CAS Key Laboratory of Marine Ecology and Environmental Sciences, Institute of Oceanology, Chinese Academy of Sciences, Qingdao 266071, China
  2.Laboratory for Marine Ecology and Environmental Science, Pilot National Laboratory for Marine Science and Technology(Qingdao), Qingdao 266237, China
  3.University of Chinese Academy of Sciences, Beijing 100049, China
  4.Center for Ocean Mega-Science, Chinese Academy of Sciences, Qingdao 266071, China
- Author bio：
  
  Rencheng YU, E-mail: rcyu@qdio.ac.cn
- Funds：
- DOI：10.1007/s00343-022-2301-0
  CLC：
- Received：17 August 2022，
  
  Accepted：15 September 2022，
  
  Online First：30 September 2022，
  
  Published：2022-11
- Accepted：
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Huixia GENG, Hongxiao SUN, Chao LIU, et al. Screening for lipophilic marine toxins and their potential producers in coastal waters of Weihai in autumn, 2020[J]. Journal of Oceanology and Limnology, 2022, 40(6): 2218-2230.
DOI：

Huixia GENG, Hongxiao SUN, Chao LIU, et al. Screening for lipophilic marine toxins and their potential producers in coastal waters of Weihai in autumn, 2020[J]. Journal of Oceanology and Limnology, 2022, 40(6): 2218-2230. DOI： 10.1007/s00343-022-2301-0.

摘要

Abstract

Lipophilic marine toxins (LMTs) produced by some microalgae in the sea could accumulate in shellfish and pose potential threats to the health of seafood consumers. Phytoplankton and shellfish samples were collected from coastal waters of Weihai in Shandong Peninsula

China in autumn

2020

and screened for lipophilic marine toxins and their potential producers using liquid chromatography-tandem mass spectrometry (LC-MS/MS) analysis and high throughput sequencing of partial DNA (V4 region of the 18S rRNA gene) extracted from phytoplankton. Pectenotoxin-2 (PTX2)

trace amounts of azaspiracid (AZA1 or AZA40)

and 13-desmethyl spirolide C (13-DesMe-C) were detected in phytoplankton samples

while PTX2 and gymnodimine (GYM) were detected in shellfish samples. The toxin content in shellfish samples was much lower than the regulatory limit or values reported previously. Results suggest that lipophilic marine toxins should have low risk in coastal waters of Weihai in autumn. Based on the data of high throughput sequencing

the OTUs were assigned to 5 identified species of

Alexandrium

including

A. ostenfeldii

capable of producing 13-DesMe-C and GYM. Two OTUs were found closely related to the toxic species in genus

Dinophysis

but it is impossible to assign them to any identified species due to the low resolving power of the V4 region for

Dinophysis

. The OTUs could not be assigned to any identified species in the genus

Azadinium

suggesting the existence of unidentified species in this region.

关键词

Keywords

references

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