Screening of nanobody against<italic style="font-style: italic">Microcystis</italic>from a human phage display nanobody library

Yao ZU; Wenjie MIAO; Yu LUO; Chen XU; Qianhui PAN; Siyu CHEN; Jianhong LI

doi:10.1007/s00343-022-1361-5

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Screening of nanobody againstMicrocystisfrom a human phage display nanobody library

Special Section: Cyanobacterial blooms in China: ecology, toxicity, and treatment | Updated：2023-05-19

- Screening of nanobody againstMicrocystisfrom a human phage display nanobody library
- Journal of Oceanology and Limnology Vol. 40, Issue 5, Pages: 1696-1705(2022)
- Affiliations：
  
  School of Life Sciences, Nanjing Normal University, Nanjing 210046, China
- Author bio：
  
  Jianhong LI,lijianhong@njnu.edu.cn;516389186@qq.com
- Funds：
- DOI：10.1007/s00343-022-1361-5
  CLC：
- Received：29 October 2021，
  
  Accepted：07 December 2021，
  
  Online First：21 January 2022，
  
  Published：2022-09
- Accepted：
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Yao ZU, Wenjie MIAO, Yu LUO, et al. Screening of nanobody againstMicrocystisfrom a human phage display nanobody library[J]. Journal of Oceanology and Limnology, 2022, 40(5): 1696-1705.
DOI：

Yao ZU, Wenjie MIAO, Yu LUO, et al. Screening of nanobody againstMicrocystisfrom a human phage display nanobody library[J]. Journal of Oceanology and Limnology, 2022, 40(5): 1696-1705. DOI： 10.1007/s00343-022-1361-5.

摘要

Abstract

Microcystis

species identification is essential for ecological studies and water bloom control. Immunoassays are more specific and convenient and several approaches have been used to develop for diagnosing harmful red tide algae. However

investigations on

Microcystis

identification using immunological approaches are still in the initial stage. In this study

Microcystis aeruginosa

PCC7806 lysates were utilized as coated antigens to enrich and screen specific

Microcystis

nanobodies from a human domain antibody display library. After three rounds of enrichment

10 positive monoclonal particles were isolated from the library and the most two positive nanobodies (DAb2 and Dab3) were effectively produced in

Escherichia coli

BL21. Finally

the DAb2 showed specific immune binding to different

Microcystis

by the immuno-dot blot assay. This antibody could be used to establish an immunological method to identify

Microcystis

关键词

Keywords

references

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Screening of nanobody againstMicrocystisfrom a human phage display nanobody library

DOI：10.1007/s00343-022-1361-5

摘要

Abstract

关键词

Keywords

references