Molecular identification, expression pattern, and in-vitro bioactivity analysis of insulin-like growth factor 2 in olive flounder <italic style="font-style: italic">Paralichthys olivaceus</italic>

Shuang JIAO; Xungang TAN; Feng YOU; Shujing ZHANG; Qiuxiang PANG

doi:10.1007/s00343-022-1358-0

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Molecular identification, expression pattern, and in-vitro bioactivity analysis of insulin-like growth factor 2 in olive flounder Paralichthys olivaceus

Biology | Updated：2023-04-27

- Molecular identification, expression pattern, and in-vitro bioactivity analysis of insulin-like growth factor 2 in olive flounder Paralichthys olivaceus
- Journal of Oceanology and Limnology Vol. 41, Issue 1, Pages: 280-289(2023)
- Affiliations：
  
  1.Anti-Aging & Regenerative Medicine Research Institution, School of Life Sciences and Medicine, Shandong University of Technology, Zibo 255000, China
  2.CAS and Shandong Province Key Laboratory of Experimental Marine Biology, Center for Ocean Mega-Science, Institute of Oceanology, Chinese Academy of Sciences, Qingdao 266071, China
  3.Laboratory for Marine Biology and Biotechnology, Pilot National Laboratory for Marine Science and Technology(Qingdao), Qingdao 266237, China
- Author bio：
  
  Shuang JIAO, E-mail: jiaoshuang@sdut.edu.cn
  Xungang TAN, E-mail: tanx@qdio.ac.cn
- Funds：
- DOI：10.1007/s00343-022-1358-0
  CLC：
- Received：29 October 2021，
  
  Accepted：06 December 2021，
  
  Online First：07 January 2022，
  
  Published：2023-01
- Accepted：
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Shuang JIAO, Xungang TAN, Feng YOU, et al. Molecular identification, expression pattern, and in-vitro bioactivity analysis of insulin-like growth factor 2 in olive flounder Paralichthys olivaceus[J]. Journal of Oceanology and Limnology, 2023, 41(1): 280-289.
DOI：

Shuang JIAO, Xungang TAN, Feng YOU, et al. Molecular identification, expression pattern, and in-vitro bioactivity analysis of insulin-like growth factor 2 in olive flounder Paralichthys olivaceus[J]. Journal of Oceanology and Limnology, 2023, 41(1): 280-289. DOI： 10.1007/s00343-022-1358-0.

摘要

Abstract

Insulin-like growth factors (IGFs) are key regulators of development and growth. Here

we characterized the

igf2

gene from olive flounder (

Paralichthys olivaceus

) and determined its temporal and spatial expression. We set up an in-vitro protein expression system in eukaryotic human embryonic kidney (HEK293T) cells and explored its effects on cell proliferation. The flounder

igf2

cDNA contained a 648-bp open reading frame (ORF) encoding a protein of 215 amino acids (aa)

which spanned the complete signal peptide (47 aa)

mature peptide (70 aa)

and E domain (98 aa). In adult flounder

igf2

mRNA was detected in all selected tissues. In early development

igf2

mRNA was detected throughout development from unfertilized eggs to hatching-stage embryos. In-situ hybridization analysis indicated that

igf2

mRNA was specially expressed in the brain region

floor plate

hypochord

otic vesicle

and pectoral fin during embryogenesis. Western blotting analysis indicated that the soluble recombinant flounder IGF2 protein was successfully produced through eukaryotic expression in HEK293T cells. In addition

the recombinant IGF2 protein significantly promoted the proliferation of human cervical carcinoma (HeLa) and HEK293T cells. These results provide new information about the structural and functional conservation

expression patterns

and biological activity of the

igf2

in teleosts.

关键词

Keywords

references

F G Ayson , E G De Jesus , S Moriyama , 等 . Differential expression of insulin-like growth factor Ⅰ and Ⅱ mRNAs during embryogenesis and early larval development in rabbitfish, Siganus guttatus . General and Comparative Endocrinology , 2002 . 126 ( 2 ): 165 - 174 . DOI: 10.1006/gcen.2002.7788 http://doi.org/10.1006/gcen.2002.7788 .

K Bäck , C Brännmark , P Strålfors , 等 . Differential effects of IGF-Ⅰ, IGF-Ⅱ and insulin in human preadipocytes and adipocytes--role of insulin and IGF-Ⅰ receptors . Molecular and Cellular Endocrinology , 2011 . 339 ( 1-2 ): 130 - 135 . DOI: 10.1016/j.mce.2011.04.005 http://doi.org/10.1016/j.mce.2011.04.005 .

S C Bendall , M H Stewart , P Menendez , 等 . IGF and FGF cooperatively establish the regulatory stem cell niche of pluripotent human cells in vitro . Nature , 2007 . 448 ( 7157 ): 1015 - 1021 . DOI: 10.1038/nature06027 http://doi.org/10.1038/nature06027 .

A L Brown , D E Graham , S P Nissley , 等 . Developmental regulation of insulin-like growth factor Ⅱ mRNA in different rat tissues . Journal of Biological Chemistry , 1986 . 261 ( 28 ): 13144 - 13150 . DOI: 10.1016/S0021-9258(18)69282-8 http://doi.org/10.1016/S0021-9258(18)69282-8 .

A Caelers , G Berishvili , M L Meli , 等 . Establishment of a real-time RT-PCR for the determination of absolute amounts of IGF-Ⅰ and IGF-Ⅱ gene expression in liver and extrahepatic sites of the tilapia . General and Comparative Endocrinology , 2004 . 137 ( 2 ): 196 - 204 . DOI: 10.1016/j.ygcen.2004.03.006 http://doi.org/10.1016/j.ygcen.2004.03.006 .

J Y Chen , C Y Chang , J C Chen , 等 . Production of biologically active recombinant tilapia insulin-like growth factor-Ⅱ polypeptides in Escherichia coli cells and characterization of the genomic structure of the coding region . DNA and Cell Biology , 1997 . 16 ( 7 ): 883 - 892 . DOI: 10.1089/dna.1997.16.883 http://doi.org/10.1089/dna.1997.16.883 .

M Codina , D G De La Serrana , J Sánchez-Gurmaches , 等 . Metabolic and mitogenic effects of IGF-Ⅱ in rainbow trout ( Oncorhynchus mykiss ) myocytes in culture and the role of IGF-Ⅱ in the PI3K/Akt and MAPK signalling pathways . General and Comparative Endocrinology , 2008 . 157 ( 2 ): 116 - 124 . DOI: 10.1016/j.ygcen.2008.04.009 http://doi.org/10.1016/j.ygcen.2008.04.009 .

A Denley , L J Cosgrove , G W Booker , 等 . Molecular interactions of the IGF system . Cytokine & Growth Factor Reviews , 2005 . 16 ( 4-5 ): 421 - 439 . DOI: 10.1016/j.cytogfr.2005.04.004 http://doi.org/10.1016/j.cytogfr.2005.04.004 .

C M Duan , J Ding , Q Li , 等 . Insulin-like growth factor binding protein 2 is a growth inhibitory protein conserved in zebrafish . Proceedings of the National Academy of Sciences of the United States of America , 1999 . 96 ( 26 ): 15274 - 15279 . DOI: 10.1073/pnas.96.26.15274 http://doi.org/10.1073/pnas.96.26.15274 .

C M Duan , H X Ren , S Gao . Insulin-like growth factors (IGFs), IGF receptors, and IGF-binding proteins: roles in skeletal muscle growth and differentiation . General and Comparative Endocrinology , 2010 . 167 ( 3 ): 344 - 351 . DOI: 10.1016/j.ygcen.2010.04.009 http://doi.org/10.1016/j.ygcen.2010.04.009 .

D E Graham , M M Rechler , A L Brown , 等 . Coordinate developmental regulation of high and low molecular weight mRNAs for rat insulin-like growth factor Ⅱ . Proceedings of the National Academy of Sciences of the United States of America , 1986 . 83 ( 12 ): 4519 - 4523 . DOI: 10.1073/pnas.83.12.4519 http://doi.org/10.1073/pnas.83.12.4519 .

M W Greene , T T Chen . Temporal expression pattern of insulin-like growth factor mRNA during embryonic development in a teleost, rainbow trout ( Onchorynchus mykiss ) . Molecular Marine Biology and Biotechnology , 1997 . 6 ( 2 ): 144 - 151 . .

T A Hall . BioEdit: a user-friendly biological sequence alignment editor and analysis program for Windows 95/98/NT . Nucleic Acids Symposium Series , 1999 . 41 95 - 98 . .

S Jiao , X G Tan , Q Wang , 等 . The olive flounder ( Paralichthys olivaceus ) Pax3 homologues are highly conserved, encode multiple isoforms and show unique expression patterns . Comparative Biochemistry and Physiology Part B: Biochemistry and Molecular Biology , 2015 . 180 7 - 15 . DOI: 10.1016/j.cbpb.2014.10.002 http://doi.org/10.1016/j.cbpb.2014.10.002 .

J I Jones , D R Clemmons . Insulin-like growth factors and their binding proteins: biological actions . Endocrine Reviews , 1995 . 16 ( 1 ): 3 - 34 . DOI: 10.1210/edrv-16-1-3 http://doi.org/10.1210/edrv-16-1-3 .

S Kumar , G Stecher , M Li , 等 . MEGA X: Molecular Evolutionary Genetics Analysis across computing platforms . Molecular Biology and Evolution , 2018 . 35 ( 6 ): 1547 - 1549 . DOI: 10.1093/molbev/msy096 http://doi.org/10.1093/molbev/msy096 .

A J Latimer , B Appel . Notch signaling regulates midline cell specification and proliferation in zebrafish . Developmental Biology , 2006 . 298 ( 2 ): 392 - 402 . DOI: 10.1016/j.ydbio.2006.05.039 http://doi.org/10.1016/j.ydbio.2006.05.039 .

J E Lee , J Pintar , A Efstratiadis . Pattern of the insulin-like growth factor Ⅱ gene expression during early mouse embryogenesis . Development , 1990 . 110 ( 1 ): 151 - 159 . DOI: 10.1242/dev.110.1.151 http://doi.org/10.1242/dev.110.1.151 .

J Z Li , P Wu , Y Liu , 等 . Temporal and spatial expression of the four Igf ligands and two Igf type 1 receptors in zebrafish during early embryonic development . Gene Expression Patterns , 2014 . 15 ( 2 ): 104 - 111 . DOI: 10.1016/j.gep.2014.05.006 http://doi.org/10.1016/j.gep.2014.05.006 .

S Moriyama , F G Ayson , H Kawauchi . Growth regulation by insulin-like growth factor-Ⅰ in fish . Bioscience, Biotechnology, and Biochemistry , 2000 . 64 ( 8 ): 1553 - 1562 . DOI: 10.1271/bbb.64.1553 http://doi.org/10.1271/bbb.64.1553 .

S Moriyama , K Yamaguchi , T Takasawa , 等 . Identification of two insulin-like growth factor Ⅱs in the Japanese eel, Anguilla japonica : cloning, tissue distribution, and expression after growth hormone treatment and seawater acclimation . Comparative Biochemistry and Physiology Part B: Biochemistry and Molecular Biology , 2008 . 149 ( 1 ): 47 - 57 . DOI: 10.1016/j.cbpb.2007.08.005 http://doi.org/10.1016/j.cbpb.2007.08.005 .

M Nipkow , E Wirthgen , P Luft , 等 . Characterization of igf1 and igf2 genes during maraena whitefish ( Coregonus maraena ) ontogeny and the effect of temperature on embryogenesis and igf expression . Growth Hormone & IGF Research , 2018 . 40 32 - 43 . DOI: 10.1016/j.ghir.2018.04.003 http://doi.org/10.1016/j.ghir.2018.04.003 .

S D O'Dell , I N Day . Molecules in focus Insulin-like growth factor Ⅱ (IGF-Ⅱ) . The International Journal of Biochemistry & Cell Biology , 1998 . 30 ( 7 ): 767 - 771 . DOI: 10.1016/s1357-2725(98)00048-x http://doi.org/10.1016/s1357-2725(98)00048-x .

L Perez , J B Ortiz-Delgado , M Manchado . Molecular characterization and transcriptional regulation by GH and GnRH of insulin-like growth factors Ⅰ and Ⅱ in white seabream ( Diplodus sargus ) . Gene , 2016 . 578 ( 2 ): 251 - 262 . DOI: 10.1016/j.gene.2015.12.030 http://doi.org/10.1016/j.gene.2015.12.030 .

V Perrot , E B Moiseeva , Y Gozes , 等 . Ontogeny of the insulin-like growth factor system (IGF-Ⅰ, IGF-Ⅱ, and IGF-1R) in gilthead seabream ( Sparus aurata ): expression and cellular localization . General and Comparative Endocrinology , 1999 . 116 ( 3 ): 445 - 460 . DOI: 10.1006/gcen.1999.7337 http://doi.org/10.1006/gcen.1999.7337 .

B C Peterson , B G Bosworth , A L Bilodeau . Differential gene expression of IGF-Ⅰ, IGF-Ⅱ, and toll-like receptors 3 and 5 during embryogenesis in hybrid (channel×blue) and channel catfish . Comparative Biochemistry and Physiology Part A: Molecular & Integrative Physiology , 2005 . 141 ( 1 ): 42 - 47 . DOI: 10.1016/j.cbpb.2005.03.009 http://doi.org/10.1016/j.cbpb.2005.03.009 .

A L Pierce , J P Breves , S Moriyama , 等 . Differential regulation of Igf1 and Igf2 mRNA levels in tilapia hepatocytes: effects of insulin and cortisol on GH sensitivity . Journal of Endocrinology , 2011 . 211 ( 2 ): 201 - 210 . DOI: 10.1530/JOE-10-0456 http://doi.org/10.1530/JOE-10-0456 .

M Placzek , J Briscoe . The floor plate: multiple cells, multiple signals . Nature Reviews Neuroscience , 2005 . 6 ( 3 ): 230 - 240 . DOI: 10.1038/nrn1628 http://doi.org/10.1038/nrn1628 .

G Radaelli , M Patruno , L Maccatrozzo , 等 . Expression and cellular localization of insulin-like growth factor-Ⅱ protein and mRNA in Sparus aurata during development . Journal of Endocrinology , 2003 . 178 ( 2 ): 285 - 299 . DOI: 10.1677/joe.0.1780285 http://doi.org/10.1677/joe.0.1780285 .

M Reinecke , B T Björnsson , W W Dickhoff , 等 . Growth hormone and insulin-like growth factors in fish: where we are and where to go . General and Comparative Endocrinology , 2005 . 142 ( 1-2 ): 20 - 24 . DOI: 10.1016/j.ygcen.2005.01.016 http://doi.org/10.1016/j.ygcen.2005.01.016 .

M Reinecke . Insulin-like growth factors and fish reproduction . Biology of Reproduction , 2010 . 82 ( 4 ): 656 - 661 . DOI: 10.1095/biolreprod.109.080093 http://doi.org/10.1095/biolreprod.109.080093 .

P Rotwein . Structure, evolution, expression and regulation of insulin-like growth factors Ⅰ and Ⅱ . Growth Factors , 1991 . 5 ( 1 ): 3 - 18 . DOI: 10.3109/08977199109000267 http://doi.org/10.3109/08977199109000267 .

M B Soares , D N Ishii , A Efstratiadis . Developmental and tissue-specific expression of a family of transcripts related to rat insulin-like growth factor Ⅱ mRNA . Nucleic Acids Research , 1985 . 13 ( 4 ): 1119 - 1134 . DOI: 10.1093/nar/13.4.1119 http://doi.org/10.1093/nar/13.4.1119 .

Sussenbach J S, Rodenburg R J T, Scheper W et al. 1993. Transcriptional and post-transcriptional regulation of the human IGF-Ⅱ gene expression. In : Le Roith D, Raizada M K eds. Current Directions in Insulin-Like Growth Factor Research. Springer, Boston, p. 63-71, https://doi.org/10.1007/978-1-4615-2988-0_7 https://doi.org/10.1007/978-1-4615-2988-0_7 .

M C L Tse , Q P Vong , C H K Cheng , 等 . PCR-cloning and gene expression studies in common carp ( Cyprinus carpio ) insulin-like growth factor-Ⅱ . Biochimica et Biophysica Acta , 2002 . 1575 ( 1-3 ): 63 - 74 . DOI: 10.1016/s0167-4781(02)00244-0 http://doi.org/10.1016/s0167-4781(02)00244-0 .

B Wang , Y J Xu , X Z Liu , 等 . Molecular characterization and expression profiles of insulin-like growth factors in yellowtail kingfish ( Seriola lalandi ) during embryonic development . Fish Physiology and Biochemistry , 2019a . 45 ( 1 ): 375 - 390 . DOI: 10.1007/s10695-018-0570-5 http://doi.org/10.1007/s10695-018-0570-5 .

C Wang , X M Li , H X Dang , 等 . Insulin-like growth factor 2 regulates the proliferation and differentiation of rat adipose-derived stromal cells via IGF-1R and IR . Cytotherapy , 2019b . 21 ( 6 ): 619 - 630 . DOI: 10.1016/j.jcyt.2018.11.010 http://doi.org/10.1016/j.jcyt.2018.11.010 .

Y A White , J T Kyle , A W Wood . Targeted gene knockdown in zebrafish reveals distinct intraembryonic functions for insulin-like growth factor Ⅱ signaling . Endocrinology , 2009 . 150 ( 9 ): 4366 - 4375 . DOI: 10.1210/en.2009-0356 http://doi.org/10.1210/en.2009-0356 .

A W Wood , C M Duan , H A Bern . Insulin-like growth factor signaling in fish . International Review of Cytology , 2005 . 243 215 - 285 . DOI: 10.1016/S0074-7696(05)43004-1 http://doi.org/10.1016/S0074-7696(05)43004-1 .

Y J Xu , K Zang , X Z Liu , 等 . Insulin-like growth factors Ⅰ and Ⅱ in starry flounder ( Platichthys stellatus ): molecular cloning and differential expression during embryonic development . Fish Physiology and Biochemistry , 2015 . 41 ( 1 ): 139 - 152 . DOI: 10.1007/s10695-014-0012-y http://doi.org/10.1007/s10695-014-0012-y .

S M Zou , H Kamei , Z Modi , 等 . Zebrafish IGF genes: gene duplication, conservation and divergence, and novel roles in midline and notochord development . PLoS One , 2009 . 4 ( 9 ): e7026 DOI: 10.1371/journal.pone.0007026 http://doi.org/10.1371/journal.pone.0007026 .

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School of Biological Sciences, University of Portsmouth, Portsmouth PO1 2DY, United Kingdom

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Molecular identification, expression pattern, and in-vitro bioactivity analysis of insulin-like growth factor 2 in olive flounder Paralichthys olivaceus

DOI：10.1007/s00343-022-1358-0

摘要

Abstract

关键词

Keywords

references