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Physiological and molecular responses of invasive cyanobacterium
Raphidiopsis raciborskii to ambient phosphorus deficiency- Journal of Oceanology and Limnology Vol. 40, Issue 5, Pages: 1792-1803(2022)
- Affiliations：
Key Laboratory of Eco-environments in Three Gorges Reservoir Region (Ministry of Education), Chongqing Key Laboratory of Plant Ecology and Resources Research in Three Gorges Reservoir Region, School of Life Sciences, Southwest University, Chongqing 400715, China
- Author bio：
Zhongxing WU,wuzhx@swu.edu.cn
- Funds：
DOI：10.1007/s00343-022-1314-z
CLC：Received：09 October 2021，
Accepted：19 November 2021，
Online First：18 January 2022，
Published：2022-09
- Accepted：
Scan QR Code
Junqiong SHI, Shuhan HE, Lu ZHAO, et al. Physiological and molecular responses of invasive cyanobacterium
Raphidiopsis raciborskii to ambient phosphorus deficiency[J]. Journal of Oceanology and Limnology, 2022, 40(5): 1792-1803.Junqiong SHI, Shuhan HE, Lu ZHAO, et al. Physiological and molecular responses of invasive cyanobacterium
Raphidiopsis raciborskii to ambient phosphorus deficiency[J]. Journal of Oceanology and Limnology, 2022, 40(5): 1792-1803. DOI： 10.1007/s00343-022-1314-z.
摘要
Abstract
Raphidiopsis raciborskii
can cause harmful cyanobacterial blooms when concentrations of environmental phosphorus (P) are very low
thus the physiological and molecular mechanisms involved in the acclimation to P need to be characterized better. The growth
chlorophyll fluorescence
alkaline phosphatase
and expression of genes directly involved in P assimilation were compared in the
R. raciborskii
FACHB 1496 strain grown with and without inorganic P. The specific growth rate (
μ
)
Chl
a
and six fluorescence parameters (minimal fluorescence (
F
0
)
maximal fluorescence (
F
m
)
maximal variable fluorescence (
F
v
)
electron transport flux (further than
Q
A
) per RC (ET
0
/RC)
quantum yield of the electron transport in PSII (
Ø
E0
)
and the probability that an electron from a trapped exciton is moved into the electron transport chain beyond
Q
A
−
(
ψ
0
)) markedly decreased in
R. raciborskii
in response to experimental P-deficiency. In contrast
the relative variable fluorescence at the J-step (
V
J
)
trapped energy flux (leading to
Q
A
reduction) per RC (TR
0
/RC)
and alkaline phosphatase activity significantly increased. In addition
gene expressions involved in the alkaline phosphatase (
phoA1
and
phoA2
)
high-affinity inorganic P transporter (
pstS1
)
phosphonate transporter and metabolism (
phnD
and
phnM
)
and nucleotidase (
nucH
) were significantly upregulated under P deficiency. However
physiological and molecular responses were resumed rapidly after P re-supplementation following P-deficient conditions. Our results highlight that
R. raciborskii
can perform coordinated and complex cellular and physiological responses to cope with P deficiency
reflecting
R. raciborskii'
s multi-faceted machinery to respond to environmental P fluctuations
关键词
Keywords
references
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