16S rRNA gene sequencing analysis on changes in the intestinal flora of<italic style="font-style: italic">Procambarus clarkii</italic>with "Black May" disease

Jie GONG; Guoqing SHEN; Mengru ZHU; Ming ZHAN; Changjun XI; Yan SHUI; Zenghong XU; Huaishun SHEN

doi:10.1007/s00343-021-1278-4

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16S rRNA gene sequencing analysis on changes in the intestinal flora ofProcambarus clarkiiwith "Black May" disease

Aquaculture and Fisheries | Updated：2023-05-19

- 16S rRNA gene sequencing analysis on changes in the intestinal flora ofProcambarus clarkiiwith "Black May" disease
- Journal of Oceanology and Limnology Vol. 40, Issue 5, Pages: 2068-2079(2022)
- Affiliations：
  
  1.Wuxi Fisheries College, Nanjing Agricultural University, Nanjing 210095, China
  2.Key Laboratory of Freshwater Fisheries and Germplasm Resources Utilization, Ministry of Agriculture, Freshwater Fisheries Research Center, Chinese Academy of Fishery Sciences, Wuxi 214081, China
- Author bio：
  
  Huaishun SHEN,shenhuaishun@ffrc.cn
- Funds：
- DOI：10.1007/s00343-021-1278-4
  CLC：
- Received：03 September 2021，
  
  Accepted：08 October 2021，
  
  Online First：26 November 2021，
  
  Published：2022-09
- Accepted：
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Jie GONG, Guoqing SHEN, Mengru ZHU, et al. 16S rRNA gene sequencing analysis on changes in the intestinal flora ofProcambarus clarkiiwith "Black May" disease[J]. Journal of Oceanology and Limnology, 2022, 40(5): 2068-2079.
DOI：

Jie GONG, Guoqing SHEN, Mengru ZHU, et al. 16S rRNA gene sequencing analysis on changes in the intestinal flora ofProcambarus clarkiiwith "Black May" disease[J]. Journal of Oceanology and Limnology, 2022, 40(5): 2068-2079. DOI： 10.1007/s00343-021-1278-4.

摘要

Abstract

The morbidity and mortality peak of farmed

Procambarus clarkii

occurs around May every year

a phenomenon known as "Black May" disease (BMD). Increasing evidence shows that the intestinal flora is closely related to host health. We analyzed and compared the microbiota of healthy and BMD-affected

clarkii

intestines. The results show that there was no significant difference in bacterial α-diversity (richness

=0.59; evenness

=0.43; and diversity

=0.052) between the diseased group and the control group. Four dominant phyla in the intestines of crayfish in the control group

namely Tenericutes (30.86%)

Bacteroidetes (29.99%)

Firmicutes (22.23%)

and Proteobacteria (15.23%)

were identified. However

a striking shift in the microbial composition were found in the intestines of

clarkii

with BMD. Bacteroidetes was a dominant phylum in healthy

clarkii

whereas the prevalence was low in diseased

clarkii

(1.87%). By contrast

the prevalence of Proteobacteria was significantly higher (

0.05) in

clarkii

with BMD than in

clarkii

without BMD.

Candidatus Bacilloplasma

Bacteroides

Vibrio

and

Aeromonas

showed significant differences (

0.05) at the genus level. Tax4Fun function prediction indicated that the relative abundance of genes involved in energy metabolism in the intestinal flora of

clarkii

with BMD was significantly reduced (

0.05). Therefore

BMD can change the composition of the intestinal microbiota of

clarkii

. This study contributes to the understanding of the relationship between intestinal flora and host especially in aquatic animals.

关键词

Keywords

references

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16S rRNA gene sequencing analysis on changes in the intestinal flora ofProcambarus clarkiiwith "Black May" disease

DOI：10.1007/s00343-021-1278-4

摘要

Abstract

关键词

Keywords

references