Involvement of the ammonium assimilation mediated by glutamate dehydrogenase in response to heat stress in the scleractinian coral<italic style="font-style: italic">Pocillopora damicornis</italic>

Jia TANG; Wenqi CAI; Zhicong YAN; Zhongjie WU; Qianxi YANG; Zhi ZHOU

doi:10.1007/s00343-021-1266-8

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Involvement of the ammonium assimilation mediated by glutamate dehydrogenase in response to heat stress in the scleractinian coralPocillopora damicornis

Biology | Updated：2023-05-19

- Involvement of the ammonium assimilation mediated by glutamate dehydrogenase in response to heat stress in the scleractinian coralPocillopora damicornis
- Journal of Oceanology and Limnology Vol. 40, Issue 5, Pages: 2001-2011(2022)
- Affiliations：
  
  1.State Key Laboratory of Marine Resource Utilization in South China Sea, Key Laboratory of Tropical Hydrobiology and Biotechnology of Hainan Province, Hainan Aquaculture Breeding Engineering Research Center, Hainan University, Haikou 570228, China
  2.Hainan Academy of Ocean and Fisheries Sciences, Haikou 570228, China
- Author bio：
  
  WUZhongjie,289663247@qq.com
  ZHOUZhi,zhouzhi@hainanu.edu.cn
- Funds：
- DOI：10.1007/s00343-021-1266-8
  CLC：
- Received：19 August 2021，
  
  Accepted：11 October 2021，
  
  Online First：24 October 2021，
  
  Published：2022-09
- Accepted：
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Jia TANG, Wenqi CAI, Zhicong YAN, et al. Involvement of the ammonium assimilation mediated by glutamate dehydrogenase in response to heat stress in the scleractinian coralPocillopora damicornis[J]. Journal of Oceanology and Limnology, 2022, 40(5): 2001-2011.
DOI：

Jia TANG, Wenqi CAI, Zhicong YAN, et al. Involvement of the ammonium assimilation mediated by glutamate dehydrogenase in response to heat stress in the scleractinian coralPocillopora damicornis[J]. Journal of Oceanology and Limnology, 2022, 40(5): 2001-2011. DOI： 10.1007/s00343-021-1266-8.

摘要

Abstract

Glutamate dehydrogenase (GDH) plays an important role in the ammonium assimilation and nitrogen metabolism by catalyzing the reversible oxidative deamination of L-glutamate to α-ketoglutarate. In the present study

the potential functions of GDH in response to heat stress were explored in the scleractinian coral

Pocillopora damicornis

(designated as PdGDH). The cDNA of PdGDH contained an open reading frame of 1 611 bp encoding a polypeptide of 536 amino acids

which exhibited the highest sequence identity to GDH of

Stylophora pistillata

(96% identity)

and the deduced PdGDH protein was predicted to contain one GdhA domain (from Val95 to Tyr525). The recombinant protein of PdGDH (rPdGDH) was expressed in

Escherichia coli

BL21 (DE3)-Transetta

and its catalytic activity was measured under diﬀerent temperatures

pH conditions and epigallocatechin-3-gallate (EGCG

a GDH inhibitor) concentrations. The purified rPdGDH only used reduced coenzyme nicotinamide adenine dinucleotide (NADH) as coenzyme

and its highest activity was observed at 35 ℃ and pH 7.5

respectively. The rPdGDH activity was negatively correlated with the concentration of EGCG

and was inhibited by more than half (65%

0.05) at 10-4 mol/L EGCG. No significant alteration of PdGDH mRNA expression was detected at 12 h after exposure to heat and ammonium (

0.05). Furthermore

the activities of NADH-GDH in the scleractinian coral

damicornis

increased significantly at 12 h after the heat and ammonium stress

and the NADH-GDH activity in the heat stress group (32.66 U/mg

0.05) was significantly higher than that in the heat and ammonium stress group (11.26 U/mg). These results collectively suggested that PdGDH

as a homologue of glutamate dehydrogenase in the scleractinian coral

damicornis

could respond to heat stress at the protein level

which would have ability to further promote ammonium assimilation to increase the heat acclimatization of the coral-Symbiodiniaceae symbiotic association.

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Keywords

references

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Involvement of the ammonium assimilation mediated by glutamate dehydrogenase in response to heat stress in the scleractinian coralPocillopora damicornis

DOI：10.1007/s00343-021-1266-8

摘要

Abstract

关键词

Keywords

references