Expression profile of long non-coding RNAs in the intestine of black rockfish <italic style="font-style: italic">Sebastes schlegelii</italic> in response to <italic style="font-style: italic">Edwardsiella tarda</italic> infection

Xu YAN; Min CAO; Qiang FU; Ning YANG; Ningning WANG; Lin SONG; Chao LI

doi:10.1007/s00343-021-1230-7

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Expression profile of long non-coding RNAs in the intestine of black rockfish Sebastes schlegelii in response to Edwardsiella tarda infection

Aquaculture and Fisheries | Updated：2023-04-27

- Expression profile of long non-coding RNAs in the intestine of black rockfish Sebastes schlegelii in response to Edwardsiella tarda infection
- Journal of Oceanology and Limnology Vol. 41, Issue 1, Pages: 376-391(2023)
- Affiliations：
  
  1.College of Chemistry and Molecular Engineering, Qingdao University of Science and Technology, Qingdao 266011, China
  2.School of Marine Science and Engineering, Qingdao Agricultural University, Qingdao 266109, China
- Author bio：
  
  Chao LI, E-mail: chaoli@qau.edu.cn
- Funds：
- DOI：10.1007/s00343-021-1230-7
  CLC：
- Received：15 July 2021，
  
  Accepted：17 September 2021，
  
  Online First：22 December 2021，
  
  Published：2023-01
- Accepted：
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Xu YAN, Min CAO, Qiang FU, et al. Expression profile of long non-coding RNAs in the intestine of black rockfish Sebastes schlegelii in response to Edwardsiella tarda infection[J]. Journal of Oceanology and Limnology, 2023, 41(1): 376-391.
DOI：

Xu YAN, Min CAO, Qiang FU, et al. Expression profile of long non-coding RNAs in the intestine of black rockfish Sebastes schlegelii in response to Edwardsiella tarda infection[J]. Journal of Oceanology and Limnology, 2023, 41(1): 376-391. DOI： 10.1007/s00343-021-1230-7.

摘要

Abstract

Long non-coding RNAs (lncRNAs) are a class of transcripts longer than 200 bp

which have been emerged as essential regulators in numerous biological processes. Black rockfish (

Sebastes schlegelii

) is an economic fish that widely cultured in the coastal areas of China

Japan

and South Korea. With the expansion of aquacultural scale

various pathogens have threatened its industry and reduced its economic values. It has been reported that lncRNA were involved in the immune response and metabolic pathway in teleost

while no study is available on identification and functional analysis of lncRNAs in black rockfish so far. Herein

this study was performed to identify lncRNAs in the intestine of black rockfish after

Edwardsiella tarda

infection. In our results

a total of 9 311 lncRNAs were identified through high-throughput sequencing

and 102 lncRNAs were significantly regulated following challenge

which were predicted to target 3 348 mRNAs. Results of Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment analyses of these target genes showed they were function in catalytic activity

hydrolase activity

defense response and peptidase activity

which involved in metabolic pathways and immune related pathways. In addition

47 lncRNAs and 8 differentially expressed mRNAs (DEmRNAs) showed co-expression at two or more infection time points with metabolism and immunity functions. Moreover

real-time quantitative PCR (qRT-PCR) was performed to verify the reliability of sequencing gene expression analysis results. This research laid the foundation for further investigation of the regulatory roles of lncRNAs in the intestinal immune response of black rockfish.

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Expression profile of long non-coding RNAs in the intestine of black rockfish Sebastes schlegelii in response to Edwardsiella tarda infection

DOI：10.1007/s00343-021-1230-7

摘要

Abstract

关键词

Keywords

references