Comparison in diversity of eukaryotic algae in surface sediments from different functional sea areas of Qingdao coast, the Yellow Sea, China: a metabarcoding approach

Zhaohui WANG; Mingdan LEI; Shuanghui JI; Changliang XIE; Jiazhuo CHEN; Weiguo LI; Tao JIANG

doi:10.1007/s00343-021-1200-0

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Comparison in diversity of eukaryotic algae in surface sediments from different functional sea areas of Qingdao coast, the Yellow Sea, China: a metabarcoding approach

Continued from the back cover | Updated：2023-05-20

- Comparison in diversity of eukaryotic algae in surface sediments from different functional sea areas of Qingdao coast, the Yellow Sea, China: a metabarcoding approach
- Journal of Oceanology and Limnology Vol. 40, Issue 6, Pages: 2322-2342(2022)
- Affiliations：
  
  1.College of Life Science and Technology, Jinan University, Guangzhou 510632, China
  2.Engineering Research Center of Tropical and Subtropical Aquatic Ecological Engineering, Ministry of Education, Guangzhou 510632, China
  3.School of Ocean, Yantai University, Yantai 264005, China
- Author bio：
  
  Zhaohui WANG, E-mail: twzh@jnu.edu.cn
  Tao JIANG, E-mail: jiangtaojnu@163.com
- Funds：
- DOI：10.1007/s00343-021-1200-0
  CLC：
- Received：22 June 2021，
  
  Accepted：11 August 2021，
  
  Online First：08 October 2021，
  
  Published：2022-11
- Accepted：
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Zhaohui WANG, Mingdan LEI, Shuanghui JI, et al. Comparison in diversity of eukaryotic algae in surface sediments from different functional sea areas of Qingdao coast, the Yellow Sea, China: a metabarcoding approach[J]. Journal of Oceanology and Limnology, 2022, 40(6): 2322-2342.
DOI：

Zhaohui WANG, Mingdan LEI, Shuanghui JI, et al. Comparison in diversity of eukaryotic algae in surface sediments from different functional sea areas of Qingdao coast, the Yellow Sea, China: a metabarcoding approach[J]. Journal of Oceanology and Limnology, 2022, 40(6): 2322-2342. DOI： 10.1007/s00343-021-1200-0.

摘要

Abstract

Surface sediment samples were collected in three different functional sea areas in Qingdao coast

East China

including the inner Jiaozhou Bay

the Laoshan Coast

and the Amphioxus Reserve area. Diversity and community structure of eukaryotic algae especially those of phytoplankton resting stages were assessed by metabarcoding V4 region of the 18S rDNA. Biogenic elements including total organic carbon (TOC)

organic matter (OM)

total nitrogen (TN)

total phosphorus (TP)

and biogenic silicon (BSi) were analyzed. A total of 1 496 eukaryotic operational taxonomic units (OTUs) were measured

including 207 algal OTUs

which contributed to 13.84% of the total OTUs. Ninety-eight species in 8 phyla

24 classes of eukaryotic algae were detected. Among them

47 species have been reported to form resting stages

and 12 species are firstly recorded in Chinese coastal waters. Dinoflagellates dominated in both DNA reads and OTU richness

which contributed to 73.02% and 61.35% of the eukaryotic algal sequences and OTU richness

respectively. DNA reads

OTU richness and alpha diversity indexes of eukaryotic algae were higher in the Laoshan Coast

and lower in Jiaozhou Bay. Eukaryotic algal community differed in the three sea areas

which was dominated by chrysophytes in Jiaozhou Bay

by dinoflagellates in the Laoshan Coast

and co-dominated by dinoflagellates and chrysophytes in the Amphioxus Reserve area. Clustering analysis showed that the Laoshan Coast and the Amphioxus Reserve area are clustered together

while Jiaozhou Bay is clustered separately. Thirty-six harmful algal bloom (HAB) species were detected

and 10 species have been reported to form blooms in Jiaozhou Bay and the Qingdao coast before. Some of these species occurred widely and dominantly in this study

suggesting high potential risk of HABs in the Qingdao coastal area.

关键词

Keywords

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