Proteomic analysis provides insights into the function of Polian vesicles in the sea cucumber<italic style="font-style: italic">Apostichopus japonicus</italic>post-evisceration

Jinlin JI; Zhenhui WANG; Wei ZHU; Qiang LI; Yinan WANG

doi:10.1007/s00343-021-1198-3

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Proteomic analysis provides insights into the function of Polian vesicles in the sea cucumberApostichopus japonicuspost-evisceration

Biology | Updated：2023-05-19

- Proteomic analysis provides insights into the function of Polian vesicles in the sea cucumberApostichopus japonicuspost-evisceration
- Journal of Oceanology and Limnology Vol. 40, Issue 5, Pages: 2028-2041(2022)
- Affiliations：
  
  1.College of Marine and Biology Engineering, Yancheng Institute of Technology, Yancheng 224051, China
  2.Dalian Ocean University, Dalian 116023, China
- Author bio：
  
  WANG Yinan,wangyinan@dlou.edu.cn
- Funds：
- DOI：10.1007/s00343-021-1198-3
  CLC：
- Received：28 June 2021，
  
  Accepted：23 September 2021，
  
  Online First：04 December 2021，
  
  Published：2022-09
- Accepted：
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Jinlin JI, Zhenhui WANG, Wei ZHU, et al. Proteomic analysis provides insights into the function of Polian vesicles in the sea cucumberApostichopus japonicuspost-evisceration[J]. Journal of Oceanology and Limnology, 2022, 40(5): 2028-2041.
DOI：

Jinlin JI, Zhenhui WANG, Wei ZHU, et al. Proteomic analysis provides insights into the function of Polian vesicles in the sea cucumberApostichopus japonicuspost-evisceration[J]. Journal of Oceanology and Limnology, 2022, 40(5): 2028-2041. DOI： 10.1007/s00343-021-1198-3.

摘要

Abstract

The Polian vesicle is the main accessory structure in the water vascular system of sea cucumbers. It can function to hold water vascular fluid under slight pressure and act as a hematopoiesis

excretory

and inflammatory response organ. Being the only organ to remain after evisceration

the Polian vesicle may function in the survival and regeneration of sea cucumber. We performed Tandem Mass Tag (TMT)-based proteomics to identify how proteins in the Polian vesicle of

Apostichopus japonicus

respond to evisceration. Among the 8 453 proteins identified from vesicle samples before evisceration (PV0h) and at 6-h post-evisceration (PV6h) and 3-d post-evisceration (PV3d)

we detected 222 differentially abundant proteins (DAPs). Most of the annotated DAPs were associated with cell growth and proliferation

immune response and wound healing

substance transport and metabolism

cytoskeleton/cilia/flagella

extracellular matrix

energy production and conversion

protein synthesis and modification

and signal recognition and transduction. Compared with PV0h

fewer DAPs were identified at PV6h

and more DAPs were found at PV3d

and these DAPs were widely distributed among multiple biological processes. Our results indicate that a wide range of biological processes was induced in Polian vesicles in response to evisceration. In particular

Polian vesicles may play important roles in the restoration of coelomocytes

immune defense

and wound healing in sea cucumber. We propose that the Polian vesicle may be involved in visceral regeneration through nutrition and energy supply and by promoting dedifferentiation and migration. Together

these results provided new insights into the function of the Polian vesicle in

japonicus

post-evisceration.

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Keywords

references

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Proteomic analysis provides insights into the function of Polian vesicles in the sea cucumberApostichopus japonicuspost-evisceration

DOI：10.1007/s00343-021-1198-3

摘要

Abstract

关键词

Keywords

references