Characterization and comparison of two C-type lectins with novel key motifs from mud crab <italic style="font-style: italic">Scylla paramamosain</italic>

Boxin ZENG; Taiwei DONG; Yanting XIA; Shun YANG; Mengmeng HUANG; Hui FEI

doi:10.1007/s00343-021-1153-3

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Characterization and comparison of two C-type lectins with novel key motifs from mud crab Scylla paramamosain

Biology | Updated：2023-05-20

- Characterization and comparison of two C-type lectins with novel key motifs from mud crab Scylla paramamosain
- Journal of Oceanology and Limnology Vol. 40, Issue 4, Pages: 1628-1639(2022)
- Affiliations：
  
  1.College of Life Sciences and Medicine, Zhejiang Sci-Tech University, Hangzhou 310018, China
  2.Zhejiang Provincial Key Laboratory of Silkworm Bioreactor and Biomedicine, Zhejiang Sci-Tech University, Hangzhou 310018, China
- Author bio：
  
  Mengmeng HUANG, E-mail: mmhuang15@zstu.edu.cn
  Hui FEI, E-mail: feihui@zstu.edu.cn
- Funds：
- DOI：10.1007/s00343-021-1153-3
  CLC：
- Received：09 June 2021，
  
  Accepted：21 July 2021，
  
  Online First：06 September 2021，
  
  Published：2022-07
- Accepted：
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Boxin ZENG, Taiwei DONG, Yanting XIA, et al. Characterization and comparison of two C-type lectins with novel key motifs from mud crab Scylla paramamosain[J]. Journal of Oceanology and Limnology, 2022, 40(4): 1628-1639.
DOI：

Boxin ZENG, Taiwei DONG, Yanting XIA, et al. Characterization and comparison of two C-type lectins with novel key motifs from mud crab Scylla paramamosain[J]. Journal of Oceanology and Limnology, 2022, 40(4): 1628-1639. DOI： 10.1007/s00343-021-1153-3.

摘要

Abstract

As an important pattern recognition receptor (PRR) in the innate immune system

C-type lectin plays an important role in the innate immune process of invertebrates. Two C-type lectins

CTL-C and

CTL-D were characterized from mud crab (

Scylla paramamosain

). The predicted

CTL-C and

CTL-D proteins both contain a single carbohydrate-recognition domain (CRD) with key motif GlnPro-Ala (QPA) and Met-Pro-Ala (MPA)

respectively.

CTL-C and

CTL-D transcripts distributed in all examined tissues

and the expression level was the highest in hepatopancreas. As PRR

the purified recombinant proteins r

CTL-C and r

CTL-D have high affinity for three kinds of pathogen-associated molecular patterns (PAMPs): β-glucan

lipopolysaccharide

and peptidoglycan. Besides

CTL-D can bind to all nine microorganisms tested

while r

CTL-C can bind to seven microorganisms except for

Staphylococcus aureus

and

Micrococcus luteus

. Both r

CTL-C and r

CTL-D showed agglutination activity towards fungi

Pichia pastoris and Saccharomyces cerevisiae

. However

CTL-C and r

CTL-D exhibited different antimicrobial activities: r

CTL-D has a certain inhibitory effect on the growth of

Vibrio fluvialis

and

M. luteus

while r

CTL-C has no obvious inhibitory activity. The results show that r

CTL-C and r

CTL-D had better phagocytosis-promoting effect on

M. luteus

than the negative control. Meanwhile

both r

CTL-C and r

CTL-D had certain encapsulation-promoting activity. Collectively

two C-type lectins with novel key motifs make an important impact as PRR in immune response towards pathogens. At the same time

they play different functions in the innate immunity of mud crab

S. paramamosain

关键词

Keywords

references

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Characterization and comparison of two C-type lectins with novel key motifs from mud crab Scylla paramamosain

DOI：10.1007/s00343-021-1153-3

摘要

Abstract

关键词

Keywords

references