Two bicistronic DNA vaccines against <italic style="font-style: italic">Vibrio anguillarum</italic> and the immune effects on flounder <italic style="font-style: italic">Paralichthys olivaceus</italic>

Hanlin LI; Jing XING; Xiaoqian TANG; Xiuzhen SHENG; Heng CHI; Wenbin ZHAN

doi:10.1007/s00343-021-1092-z

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Two bicistronic DNA vaccines against Vibrio anguillarum and the immune effects on flounder Paralichthys olivaceus

Aquaculture and Fisheries | Updated：2023-05-20

- Two bicistronic DNA vaccines against Vibrio anguillarum and the immune effects on flounder Paralichthys olivaceus
- Journal of Oceanology and Limnology Vol. 40, Issue 2, Pages: 786-804(2022)
- Affiliations：
  
  1.Laboratory of Pathology and Immunology of Aquatic Animals, KLMME, Ocean University of China, Qingdao 266003, China
  2.Laboratory for Marine Fisheries Science and Food Production Processes, Pilot National Laboratory for Marine Science and Technology(Qingdao), Qingdao 266071, China
- Author bio：
  
  Jing XING, E-mail: xingjing@ouc.edu.cn
- Funds：
- DOI：10.1007/s00343-021-1092-z
  CLC：
- Received：22 March 2021，
  
  Accepted：22 April 2021，
  
  Online First：11 May 2021，
  
  Published：2022-03
- Accepted：
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Hanlin LI, Jing XING, Xiaoqian TANG, et al. Two bicistronic DNA vaccines against Vibrio anguillarum and the immune effects on flounder Paralichthys olivaceus[J]. Journal of Oceanology and Limnology, 2022, 40(2): 786-804.
DOI：

Hanlin LI, Jing XING, Xiaoqian TANG, et al. Two bicistronic DNA vaccines against Vibrio anguillarum and the immune effects on flounder Paralichthys olivaceus[J]. Journal of Oceanology and Limnology, 2022, 40(2): 786-804. DOI： 10.1007/s00343-021-1092-z.

摘要

Abstract

Chemokines are cytokines that can promote the activation and migration of immune cells

and increase the recognition of antigen by antigen-presenting cells (APC). Previous studies showed that a DNA vaccine can induce humoral and cellular immune responses of flounder after immunization. To explore the improvement of chemokines on the efficiency of OmpK vaccine

two bicistronic DNA candidate vaccines were constructed and the immune responses they induced in the flounder were investigated by reverse transcription polymerase chain reaction (RT-PCR)

indirect immunofluorescent assay (IFA)

E staining

flow cytometry (FCM)

and quantificational real-time polymerase chain reaction (qRT-PCR). pBudCE4.1 plasmid as an expression vector

bicistronic DNA vaccines encoding OmpK gene and CC-motif ligand 4 gene (p-OmpKCCL4)

or Ompk gene and CC-motif ligand 19 gene (p-OmpK-CCL19) were successfully constructed. The results showed that two bicistronic DNA vaccines expressed Ompk protein of

Vibrio anguillarum

and CCL4/CCL19 proteins of flounder both in vitro and in vivo. After immunization

a large number of leucocytes in muscle were recruited at the injection site in treatment groups. The constructed vaccines induced significant increases in CD4-1

and CD4-2

T lymphocytes

and sIgM

B lymphocytes in peripheral blood

spleen

and head kidney. The percentage of T lymphocytes peaked on the 14

post-vaccination day whereas that of B lymphocytes peaked in the 6

post-vaccination week. Moreover

the expression profiles of 10 immune-related genes increased in muscles around the injection site

spleen

and head kidney. After the challenge

p-OmpKCCL4 and p-OmpK-CCL19 conferred a relative percentage survival (RPS) of 74.1% and 63.3%

respectively

higher than p-OmpK alone (40.8%). In conclusion

both CCL4 and CCL19 can improve the protection of p-OmpK via evoking local immune response and then humoral and cellular immunity. CCL4 and CCL19 will be potential molecular adjuvants for use in DNA vaccines.

关键词

Keywords

references

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Two bicistronic DNA vaccines against Vibrio anguillarum and the immune effects on flounder Paralichthys olivaceus

DOI：10.1007/s00343-021-1092-z

摘要

Abstract

关键词

Keywords

references