Quantitative proteomics provides insight into the response of the marine dinoflagellate <italic style="font-style: italic">Prorocentrum donghaiense</italic> to changes in ambient phosphorus

Shufeng ZHANG; Chunjuan YUAN; Ying CHEN; Lin LIN; Dazhi WANG

doi:10.1007/s00343-021-1030-0

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Quantitative proteomics provides insight into the response of the marine dinoflagellate Prorocentrum donghaiense to changes in ambient phosphorus

Ecology | Updated：2023-05-20

- Quantitative proteomics provides insight into the response of the marine dinoflagellate Prorocentrum donghaiense to changes in ambient phosphorus
- Journal of Oceanology and Limnology Vol. 40, Issue 2, Pages: 563-576(2022)
- Affiliations：
  
  1.State Key Laboratory of Marine Environmental Science/College of the Environment and Ecology, Xiamen University, Xiamen 361102, China
  2.Key Laboratory of Marine Ecology & Environmental Sciences, Institute of Oceanology, Chinese Academy of Sciences, Qingdao 266071, China
- Author bio：
  
  Dazhi WANG, E-mail: dzwang@xmu.edu.cn
- Funds：
- DOI：10.1007/s00343-021-1030-0
  CLC：
- Received：19 October 2020，
  
  Accepted：24 February 2021，
  
  Online First：11 May 2021，
  
  Published：2022-03
- Accepted：
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Shufeng ZHANG, Chunjuan YUAN, Ying CHEN, et al. Quantitative proteomics provides insight into the response of the marine dinoflagellate Prorocentrum donghaiense to changes in ambient phosphorus[J]. Journal of Oceanology and Limnology, 2022, 40(2): 563-576.
DOI：

Shufeng ZHANG, Chunjuan YUAN, Ying CHEN, et al. Quantitative proteomics provides insight into the response of the marine dinoflagellate Prorocentrum donghaiense to changes in ambient phosphorus[J]. Journal of Oceanology and Limnology, 2022, 40(2): 563-576. DOI： 10.1007/s00343-021-1030-0.

摘要

Abstract

Dinoflagellates are the major causative agents of harmful algal blooms in the global ocean and they usually form blooms under conditions of very low dissolved inorganic phosphorus (DIP). However

the mechanisms underpinning the dinoflagellate blooms remain unclear. Here

we quantitatively compared protein expression profiles of a marine dinoflagellate

Prorocentrum donghaiense

grown in inorganic P-replete

P-deficient

and DIP- and dissolved organic phosphorus (DOP)-resupplied conditions by employing a Tandem Mass Tag (TMT)-based quantitative proteomic approach. Proteins involved in intracellular P reallocation

organic P

and non-P lipid utilization were up-regulated under the P-deficient condition

while inorganic phosphate transporters varied insignificantly. In response to the P resupplementation

nitrogen metabolism

ribosome

porphyrin

and chlorophyll metabolism were up-regulated

while lysosome

and starch and sucrose metabolism were down-regulated. Notably

photosynthesis was up-regulated and secondary metabolism was down-regulated only in the DIP-resupplied cells

whereas amino acid metabolism and vitamin B6 metabolism were up-regulated in the DOP-resupplied cells

indicating differential response mechanisms of

P. donghaiense

to DIP or DOP resupplementation. Our results indicated that

P. donghaiense

initiated multiple strategies in response to an ambient inorganic P-deficiency

and its efficient DOP assimilation by providing both P and carbon sources might be a key factor driving bloom formations of

P. donghaiense

in a low DIP environment.

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Keywords

references

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Quantitative proteomics provides insight into the response of the marine dinoflagellate Prorocentrum donghaiense to changes in ambient phosphorus

DOI：10.1007/s00343-021-1030-0

摘要

Abstract

关键词

Keywords

references