Physiological and histological responses of <italic style="font-style: italic">Phascolosoma esculenta</italic> (Sipuncula: Phascolosomatidea) to acute heat stress

Xinming GAO; Haiyan Yang; Daojun Tang; Chen Du; Shan Jin; Congcong HOU; Chundan ZHANG; Junquan ZHU; Jianping Wang

doi:10.1007/s00343-021-1013-1

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Physiological and histological responses of Phascolosoma esculenta (Sipuncula: Phascolosomatidea) to acute heat stress

Article | Updated：2023-05-20

- Physiological and histological responses of Phascolosoma esculenta (Sipuncula: Phascolosomatidea) to acute heat stress
- Journal of Oceanology and Limnology Vol. 40, Issue 2, Pages: 643-655(2022)
- Affiliations：
  
  1.Key Laboratory of Applied Marine Biotechnology by the Ministry of Education, School of Marine Sciences, Ningbo University, Ningbo, 315211, China
  2.Ningbo Academy of Oceanology and Fisheries, Ningbo, 315012, China
- Author bio：
  
  Junquan ZHU, E-mail: zhujunquan@nbu.edu.cn
  Jianping WANG, E-mail: wjping805@126.com
- Funds：
- DOI：10.1007/s00343-021-1013-1
  CLC：
- Received：11 January 2021，
  
  Accepted：22 March 2021，
  
  Online First：19 April 2021，
  
  Published：2022-03
- Accepted：
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Xinming GAO, Haiyan Yang, Daojun Tang, et al. Physiological and histological responses of Phascolosoma esculenta (Sipuncula: Phascolosomatidea) to acute heat stress[J]. Journal of Oceanology and Limnology, 2022, 40(2): 643-655.
DOI：

Xinming GAO, Haiyan Yang, Daojun Tang, et al. Physiological and histological responses of Phascolosoma esculenta (Sipuncula: Phascolosomatidea) to acute heat stress[J]. Journal of Oceanology and Limnology, 2022, 40(2): 643-655. DOI： 10.1007/s00343-021-1013-1.

摘要

Abstract

Temperature is an important factor affecting the growth

development

and survival of organisms. The effects of temperature stress on aquatic organisms have received increasing attention

as these organisms are mostly poikilotherms and their body temperature are directly corresponding changes with ambient temperature

resulting in they are easily exposed in temperature stress. However

little is known about the effects of high temperature on Sipuncula. In this study

we investigated the effects of acute heat stress on malondialdehyde (MDA) concentration

the activities of antioxidant (superoxide dismutase and glutathione peroxidase) and immunity-related (acidic and alkaline phosphatase) enzymes

heat shock protein 70 (

hsp70

) and

hsp90

gene expression

and the histological structure of the sipunculid

Phascolosoma esculenta

. Within the coelom fluid

the MDA concentration and all detected antioxidant enzyme activities increased during high temperature stress; significant increases were also observed here and in the intestine in the

hsp70

and

hsp90

mRNA expression levels. These results indicated that acute heat stress caused oxidative stress; antioxidants and heat shock proteins probably act to protect

P. esculenta

against oxidative damage

constituting part of its physiological mechanism for adaptation to high temperatures. In addition

the increased activity of the acidic and alkaline phosphatases indicated effects on its nonspecific immune system. Furthermore

damaged tissue structures were observed in the body wall

retractor muscle

intestine

and nephridium after 96 h of 40-℃ stress. The damaged cells of these tissues showed obvious condensed chromatin around the nuclear membrane. This histological damage suggests that heat stress could affect movement

food absorption

digestion

and excretion in

P. esculenta

. These results elucidate the effects of temperature stress on

P. esculenta

and its physiological response mechanisms and provide practical indicators for assessing heat stress status and determining suitable culture temperatures for

P. esculenta

关键词

Keywords

references

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Related Institution

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CAS and Shandong Province Key Laboratory of Experimental Marine Biology, Center for Ocean Mega-Science, Institute of Oceanology, Chinese Academy of Sciences

Marine Biology and Biotechnology Laboratory, Pilot National Laboratory for Marine Science and Technology(Qingdao)

Key Laboratory of Mariculture, Ministry of Education, Ocean University of China

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⁰

Physiological and histological responses of Phascolosoma esculenta (Sipuncula: Phascolosomatidea) to acute heat stress

DOI：10.1007/s00343-021-1013-1

摘要

Abstract

关键词

Keywords

references