Diversity and community structure of eukaryotic microalgae in surface sediments in the central Bohai Sea, China, based on a metabarcoding approach

Zhaohui WANG; Chaofan WANG; Maoting WANG; Weiguo LI; Wencong ZHONG; Lei LIU; Tao JIANG

doi:10.1007/s00343-021-0481-7

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Diversity and community structure of eukaryotic microalgae in surface sediments in the central Bohai Sea, China, based on a metabarcoding approach

Dormant cells and cysts | Updated：2023-05-20

- Diversity and community structure of eukaryotic microalgae in surface sediments in the central Bohai Sea, China, based on a metabarcoding approach
- Journal of Oceanology and Limnology Vol. 40, Issue 6, Pages: 2277-2291(2022)
- Affiliations：
  
  1.College of Life Science and Technology, Jinan University, Guangzhou 510632, China
  2.Engineering Research Center of Tropical and Subtropical Aquatic Ecological Engineering, Ministry of Education, Guangzhou 510632, China
  3.School of Ocean, Yantai University, Yantai 264005, China
- Author bio：
  
  WANG Zhaohui, E-mail: twzh@jnu.edu.cn
  JIANG Tao, E-mail: jiangtaophy@163.com
- Funds：
- DOI：10.1007/s00343-021-0481-7
  CLC：
- Received：03 January 2021，
  
  Accepted：09 March 2021，
  
  Online First：25 August 2022，
  
  Published：2022-11
- Accepted：
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Zhaohui WANG, Chaofan WANG, Maoting WANG, et al. Diversity and community structure of eukaryotic microalgae in surface sediments in the central Bohai Sea, China, based on a metabarcoding approach[J]. Journal of Oceanology and Limnology, 2022, 40(6): 2277-2291.
DOI：

Zhaohui WANG, Chaofan WANG, Maoting WANG, et al. Diversity and community structure of eukaryotic microalgae in surface sediments in the central Bohai Sea, China, based on a metabarcoding approach[J]. Journal of Oceanology and Limnology, 2022, 40(6): 2277-2291. DOI： 10.1007/s00343-021-0481-7.

摘要

Abstract

Sediment samples were collected at 17 stations in the central Bohai Sea

China

and the diversity and community structure of eukaryotic microalgae were assessed by metabarcoding the V4 region of 18S rDNA. A total of 930 operational taxonomic units (OTUs) were detected for microeukaryotes

including 98 algal OTUs. The algal communities comprised 42 genera belonging to 19 classes of six phyla

and they were dominated by chrysophytes and dinoflagellates. Dinoflagellates were also the most diverse microalgal group. The nano-sized dinoflagellates

Biecheleria halophila

and

Azadinium trinitatum

occurred abundantly in the study area; however

they have not been reported previously

as they may be overlooked or misidentified in light microscopy. Many pico-sized chlorophytes were detected in the sediment samples. Sixteen of the detected OTUs were assigned to potentially harmful and/or bloom-forming microalgae

suggesting some potential risks of harmful algal blooms in the central Bohai Sea. The capacity of metabarcoding to detect morphologically cryptic and small species makes this method a sufficiently sensitive means of detection for assessing eukaryotic microalgae in sediments.

关键词

Keywords

references

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