Impact of ocean acidification on physiology and microbiota in hepatopancreas of Pacific oyster <italic style="font-style: italic">Crassostrea gigas</italic>

Lingshuai Zhang; Xiudan Wang; Weiqian ZHANG; Xiaoting Yin; Qing Liu; Limei Qiu

doi:10.1007/s00343-021-0462-x

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Impact of ocean acidification on physiology and microbiota in hepatopancreas of Pacific oyster Crassostrea gigas

Article | Updated：2023-05-20

- Impact of ocean acidification on physiology and microbiota in hepatopancreas of Pacific oyster Crassostrea gigas
- Journal of Oceanology and Limnology Vol. 40, Issue 2, Pages: 620-633(2022)
- Affiliations：
  
  1.CAS and Shandong Province Key Laboratory of Experimental Marine Biology, Institute of Oceanology, Center for Ocean MegaScience, Chinese Academy of Sciences, Qingdao 266071, China
  2.Qingdao University of Science and Technology, Qingdao 266071, China
  3.University of Chinese Academy of Sciences, Beijing 100049, China
  4.Laboratory for Marine Biology and Biotechnology, Pilot National Laboratory for Marine Science and Technology (Qingdao), Qingdao 266237, China
- Author bio：
  
  Limei QIU, E-mail: qiulimei@qdio.ac.cn
- Funds：
- DOI：10.1007/s00343-021-0462-x
  CLC：
- Received：01 December 2020，
  
  Accepted：27 February 2021，
  
  Online First：14 April 2021，
  
  Published：2022-03
- Accepted：
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Lingshuai Zhang, Xiudan Wang, Weiqian ZHANG, et al. Impact of ocean acidification on physiology and microbiota in hepatopancreas of Pacific oyster Crassostrea gigas[J]. Journal of Oceanology and Limnology, 2022, 40(2): 620-633.
DOI：

Lingshuai Zhang, Xiudan Wang, Weiqian ZHANG, et al. Impact of ocean acidification on physiology and microbiota in hepatopancreas of Pacific oyster Crassostrea gigas[J]. Journal of Oceanology and Limnology, 2022, 40(2): 620-633. DOI： 10.1007/s00343-021-0462-x.

摘要

Abstract

The hepatopancreas is an important tissue involved in various biological metabolism for mollusks

but its responses to ocean acidification (OA) have not been well evaluated. In this study

the oysters were cultured in simulated conditions by continuously bubbling with ambient air (pH=8.10) or air-CO

(pH=7.50) for up to two months

and the variations on the antioxidant capacity

digestive ability

and microbiota composition in hepatopancreas of

Crassostrea gigas

were analyzed. The results show that although superoxide dismutase and glutathione responded quickly to OA stress

the antioxidant capacity of the hepatopancreas was inhibited

as revealed by the decrease of the total antioxidant capacity

which led to an upward trend of the malondialdehyde

demonstrating that the oxidative damages were accumulated under the OA process. The determination of the digestive ability manifested as the decrease of pepsin activity and the recovery of lipase and amylase activity after long-term acidification

which may be helpful to improve the adaptability of oysters. In addition

analysis on 16S rDNA amplicon revealed that the total species abundance and diversity of the hepatopancreas microbiota experienced a dynamic change

but finally it decreased greatly after long-term acidification. The structure of the hepatopancreas microbiota was changed drastically with the change of the dominant species from aerobic to the anaerobic and facultative anaerobic bacteria

and the abnormal proliferation of some species

such as genus of

Mycoplasma

and order Clostridiales

which may aggravate the adverse effects of OA on the physiological functions of the hepatopancreas. As a result

our findings enrich our understanding of the accumulated oxidative damage and adaptive digestive ability in oyster hepatopancreas caused by OA. For the first time

the changes of the hepatopancreas microbiota under long-term acidification conditions are described

proving a good reference for the study of the response and adaptation mechanisms of bivalve mollusks in a wide range of oceans OA.

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Keywords

references

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Impact of ocean acidification on physiology and microbiota in hepatopancreas of Pacific oyster Crassostrea gigas

DOI：10.1007/s00343-021-0462-x

摘要

Abstract

关键词

Keywords

references