Characterization, functional properties, and antioxidant activities of macromolecular extracts isolated from <italic style="font-style: italic">Pyropia yezoensis</italic>

WANG Xiaoqing; GENG Lihua; YUE Yang; WU Ning; ZHANG Quanbin; ZHOU Yongdong; WANG Jing

doi:10.1007/s00343-021-0456-8

Your Location：

Home >

Browse articles >

Characterization, functional properties, and antioxidant activities of macromolecular extracts isolated from Pyropia yezoensis

Biology | Updated：2023-04-27

- Characterization, functional properties, and antioxidant activities of macromolecular extracts isolated from Pyropia yezoensis
- Journal of Oceanology and Limnology Vol. 40, Issue 1, Pages: 273-283(2022)
- Affiliations：
  
  1.CAS and Shandong Province Key Laboratory of Experimental Marine Biology, Center for Ocean Mega-Science, Institute of Oceanology, Chinese Academy of Sciences, Qingdao 266071, China
  2.Laboratory for Marine Biology and Biotechnology, Pilot National Laboratory for Marine Science and Technology (Qingdao), Qingdao 266237, China
  3.University of Chinese Academy of Sciences, Beijing 100049, China
  4.Jiangsu Dongshang Eslite Co. Ltd., Yancheng 224145, China
- Author bio：
  
  Jing WANG, jingwang@qdio.ac.cn
- Funds：
- DOI：10.1007/s00343-021-0456-8
  CLC：
- Received：03 December 2020，
  
  Accepted：05 January 2021，
  
  Online First：15 January 2021，
  
  Published：2022-01
- Accepted：
Scan QR Code
WANG Xiaoqing, GENG Lihua, YUE Yang, et al. Characterization, functional properties, and antioxidant activities of macromolecular extracts isolated from Pyropia yezoensis[J]. Journal of Oceanology and Limnology, 2022, 40(1): 273-283.
DOI：

WANG Xiaoqing, GENG Lihua, YUE Yang, et al. Characterization, functional properties, and antioxidant activities of macromolecular extracts isolated from Pyropia yezoensis[J]. Journal of Oceanology and Limnology, 2022, 40(1): 273-283. DOI： 10.1007/s00343-021-0456-8.

摘要

Abstract

Pyropia yezoensis

is one of the most economical seaweed species in China. Determining how to make full use of mature

P. yezoensis

and exploring new ways to increase the value of its resources are important subjects of research for the development of the laver breeding industry. In this study

we provide a simple method to comprehensively extract the bioactive substances from

P. yezoensis

. The characteristics

functional properties

and antioxidant activities of two types of biopolymer extract from

P. yezoensis

were studied and analyzed relatively. Based on the characterization of water-soluble concentrate (WSC) and alkali-soluble concentrate (ASC)

obtained via chemical analysis

including Fourier Transform Infrared Spectrometer (FT-IR) and Thermal Gravimetric Analyzer (TGA)

and differential scanning calorimetry (DSC)

they both had typical polysaccharide and protein characteristics and steady composition. ASC showed higher nitrogen solubility

water holding capacity

and foaming ability. ASC could not only be used as a protein supplement

but also performed well in improving the properties of foods in terms of water holding and fat-absorption. The emulsifying activity and oil-holding capacity of WSC were observed to be higher than those of ASC. Thus

WSC has the potential to be used as an emulsifier. Surprisingly

WSC and ASC have radical scavenging capacity in vitro

which broadens the direction of their application.

关键词

Keywords

references

M Abdollahi , J Axelsson , N G Carlsson , G M Nylund , E Albers , I Undeland . Effect of stabilization method and freeze/thaw-aided precipitation on structural and functional properties of proteins recovered from brown seaweed (Saccharina latissima) . Food Hydrocolloids , 2019 . 96 140 - 150 . DOI: 10.1016/j.foodhyd.2019.05.007 http://doi.org/10.1016/j.foodhyd.2019.05.007 .

O Alizadeh , J Aliakbarlu . Effects of ultrasound and ohmic heating pretreatments on hydrolysis, antioxidant and antibacterial activities of whey protein concentrate and its fractions . LWT , 2020 . 131 109913 DOI: 10.1016/j.lwt.2020.109913 http://doi.org/10.1016/j.lwt.2020.109913 .

Bedoux G, Hardouin K, Burlot A S, Bourgougnon N. 2014. Bioactive components from seaweeds: cosmetic applications and future development. In : Bourgougnon N ed. Advances in Botanical Research. Academic Press, Oxford. p. 345-378.

S Benelhadj , A Gharsallaoui , P Degraeve , H Attia , D Ghorbel . Effect of pH on the functional properties of Arthrospira (Spirulina) platensis protein isolate . Food Chemistry , 2016 . 194 1056 - 1063 . DOI: 10.1016/j.foodchem.2015.08.133 http://doi.org/10.1016/j.foodchem.2015.08.133 .

M B Bera , R K Mukherjee . Solubility, emulsifying, and foaming properties of rice bran protein concentrates . Journal of Food Science , 1989 . 54 ( 1 ): 142 - 145 . DOI: 10.1111/j.1365-2621.1989.tb08587.x http://doi.org/10.1111/j.1365-2621.1989.tb08587.x .

T Bito , F Teng , F Watanabe . Bioactive compounds of edible purple laver Porphyra sp.(Nori) . Journal of Agricultural and Food Chemistry , 2017 . 65 ( 49 ): 10685 - 10692 . DOI: 10.1021/acs.jafc.7b04688 http://doi.org/10.1021/acs.jafc.7b04688 .

S Bleakley , M Hayes . Algal proteins: extraction, application, and challenges concerning production . Foods , 2017 . 6 ( 5 ): 33 DOI: 10.3390/foods6050033 http://doi.org/10.3390/foods6050033 .

N A Blouin , J A Brodie , A C Grossman , P Xu , S H Brawley . Porphyra : a marine crop shaped by stress . Trends in Plant Science , 2011 . 16 ( 1 ): 29 - 37 . DOI: 10.1016/j.tplants.2010.10.004 http://doi.org/10.1016/j.tplants.2010.10.004 .

G K Chandi , D S Sogi . Functional properties of rice bran protein concentrates . Journal of Food Engineering , 2007 . 79 ( 2 ): 592 - 597 . DOI: 10.1016/j.jfoodeng.2006.02.018 http://doi.org/10.1016/j.jfoodeng.2006.02.018 .

Y X Chen , J C Chen , C Chang , J Chen , F W Cao , J W Zhao , Y F Zheng , J J Zhu . Physicochemical and functional properties of proteins extracted from three microalgal species . Food Hydrocolloids , 2019 . 96 510 - 517 . DOI: 10.1016/j.foodhyd.2019.05.025 http://doi.org/10.1016/j.foodhyd.2019.05.025 .

X Chen , M H Wu , Q Yang , S Y Wang . Preparation, characterization of food grade phycobiliproteins from Porphyra haitanensis and the application in liposome-meat system . LWT , 2017 . 77 468 - 474 . DOI: 10.1016/j.lwt.2016.12.005 http://doi.org/10.1016/j.lwt.2016.12.005 .

P Chivero , S Gohtani , H Yoshii , A Nakamura . Physical properties of oil-in-water emulsions as a function of oil and soy soluble polysaccharide types . Food Hydrocolloids , 2014 . 39 34 - 40 . DOI: 10.1016/j.foodhyd.2013.12.017 http://doi.org/10.1016/j.foodhyd.2013.12.017 .

Damodaran S. 1997. Food proteins: an overview. In : Food Proteins and their Applications . Marcel Dekker, New York. p. 1-24.

M X Du , J H Xie , B Gong , X Xu , W Tang , X Li , C Li , M Y Xie . Extraction, physicochemical characteristics and functional properties of Mung bean protein . Food Hydrocolloids , 2018 . 76 131 - 140 . DOI: 10.1016/j.foodhyd.2017.01.003 http://doi.org/10.1016/j.foodhyd.2017.01.003 .

M DuBois , K A Gilles , J K Hamilton , P A Rebers , F Smith . Colorimetric method for determination of sugars and related substances . Analytical Chemistry , 1956 . 28 ( 3 ): 350 - 356 . DOI: 10.1021/ac60111a017 http://doi.org/10.1021/ac60111a017 .

J Emerit , M Edeas , F Bricaire . Neurodegenerative diseases and oxidative stress . Biomedicine & Pharmacotherapy , 2004 . 58 ( 1 ): 39 - 46 . https://pubmed.ncbi.nlm.nih.gov/15031734/ https://pubmed.ncbi.nlm.nih.gov/15031734/ , .

J Fleurence . Seaweed proteins: biochemical, nutritional aspects and potential uses . Trends in Food Science & Technology , 1999 . 10 ( 1 ): 25 - 28 . https://www.sciencedirect.com/science/article/pii/S0924224499000151 https://www.sciencedirect.com/science/article/pii/S0924224499000151 , .

Fleurence J. 2004. Seaweed proteins. In : Proteins in Food Processing. Woodhead Publishing, Cambridge, UK. p. 197-213.

J Fleurence , C Le Coeur , S Mabeau , M Maurice , A Landrein . Comparison of different extractive procedures for proteins from the edible seaweeds Ulva rigida and Ulva rotundata . Journal of Applied Phycology , 1995 . 7 ( 6 ): 577 - 582 . DOI: 10.1007/BF00003945 http://doi.org/10.1007/BF00003945 .

M Garcia-Vaquero , M Lopez-Alonso , M Hayes . Assessment of the functional properties of protein extracted from the brown seaweed Himanthalia elongata (Linnaeus) S.F. Gray . Food Research International , 2017 . 99 971 - 978 . DOI: 10.1016/j.foodres.2016.06.023 http://doi.org/10.1016/j.foodres.2016.06.023 .

L H Geng , J Wang , Z S Zhang , Y Yue , Q B Zhang . Structure and bioactivities of porphyrans and oligoporphyrans . Current Pharmaceutical Design , 2019 . 25 ( 11 ): 1163 - 1171 . DOI: 10.2174/1381612825666190430111725 http://doi.org/10.2174/1381612825666190430111725 .

S Honda , E Akao , S Suzuki , M Okuda , K Kakehi , J Nakamura . High-performance liquid chromatography of reducing carbohydrates as strongly ultraviolet-absorbing and electrochemically sensitive 1-phenyl-3-methyl5-pyrazolone derivatives . Analytical Biochemistry , 1989 . 180 ( 2 ): 351 - 357 . DOI: 10.1016/0003-2697(89)90444-2 http://doi.org/10.1016/0003-2697(89)90444-2 .

Hudek K, Davis L C, Ibbini J, Erickson L. 2014. Commercial products from algae. In: Algal Biorefineries. Springer, Dordrecht. p. 275-295.

S Isaka , K Cho , S Nakazono , R Abu , M Ueno , D Kim , T Oda . Antioxidant and anti-inflammatory activities of porphyran isolated from discolored nori ( Porphyra yezoensis ) . International Journal of Biological Macromolecules , 2015 . 74 68 - 75 . DOI: 10.1016/j.ijbiomac.2014.11.043 http://doi.org/10.1016/j.ijbiomac.2014.11.043 .

M Kačuráková , P Capek , V Sasinková , N Wellner , A Ebringerová . FT-IR study of plant cell wall model compounds: pectic polysaccharides and hemicelluloses . Carbohydrate Polymers , 2000 . 43 ( 2 ): 195 - 203 . DOI: 10.1016/S0144-8617(00)00151-X http://doi.org/10.1016/S0144-8617(00)00151-X .

B M Khan , H M Qiu , S Y Xu , Y Liu , K L Cheong . Physicochemical characterization and antioxidant activity of sulphated polysaccharides derived from Porphyra haitanensis . International Journal of Biological Macromolecules , 2020 . 145 1155 - 1161 . DOI: 10.1016/j.ijbiomac.2019.10.040 http://doi.org/10.1016/j.ijbiomac.2019.10.040 .

J L Kong , S N Yu . Fourier transform infrared spectroscopic analysis of protein secondary structures . Acta Biochimica et Biophysica Sinica , 2007 . 39 ( 8 ): 549 - 559 . DOI: 10.1111/j.1745-7270.2007.00320.x http://doi.org/10.1111/j.1745-7270.2007.00320.x .

H Leridon . World population outlook: explosion or implosion? . Population & Societies , 2020 . 572 1 - 4 . https://www.cairn-int.info/journal-population-and-societies-2020-1-page-1.htm https://www.cairn-int.info/journal-population-and-societies-2020-1-page-1.htm , .

S Mohamed , S N Hashim , H A Rahman . Seaweeds: a sustainable functional food for complementary and alternative therapy . Trends in Food Science & Technology , 2012 . 23 ( 2 ): 83 - 96 . https://www.sciencedirect.com/science/article/pii/S0924224411001737 https://www.sciencedirect.com/science/article/pii/S0924224411001737 , .

J F Niu , Z F Chen , G C Wang , B C Zhou . Purification of phycoerythrin from Porphyra yezoensis Ueda (Bangiales, Rhodophyta) using expanded bed absorption . Journal of Applied Phycology , 2010 . 22 ( 1 ): 25 - 31 . DOI: 10.1007/s10811-009-9420-2 http://doi.org/10.1007/s10811-009-9420-2 .

Nollet L M L, Toldrá F. 2012. Handbook of Analysis of Active Compounds in Functional Foods. CRC Press, Boca Raton, FL.

I F Olawuyi , S R Kim , D Hahn , W Y Lee . Influences of combined enzyme-ultrasonic extraction on the physicochemical characteristics and properties of okra polysaccharides . Food Hydrocolloids , 2020 . 100 105396 DOI: 10.1016/j.foodhyd.2019.105396 http://doi.org/10.1016/j.foodhyd.2019.105396 .

Patel A R. 2018. Edible foams stabilized by food-grade polymers. In: Gutiérrez T ed. Polymers for Food Applications. Springer, Cham. p. 251-269.

M N Perović , Z D Knežević Jugović , M G Antov . Improved recovery of protein from soy grit by enzyme-assisted alkaline extraction . Journal of Food Engineering , 2020 . 276 109894 DOI: 10.1016/j.jfoodeng.2019.109894 http://doi.org/10.1016/j.jfoodeng.2019.109894 .

S Poole , S I West , C L Walters . Protein-protein interactions: their importance in the foaming of heterogeneous protein systems . Journal of the Science of Food and Agriculture , 1984 . 35 ( 6 ): 701 - 711 . DOI: 10.1002/jsfa.2740350618 http://doi.org/10.1002/jsfa.2740350618 .

W Qu , H L Ma , T Wang , H Zheng . Alternating two-frequency countercurrent ultrasonic-assisted extraction of protein and polysaccharide from Porphyra yezoensis . Transactions of the Chinese Society of Agricultural Engineering , 2013 . 29 ( 1 ): 285 - 292 . https://www.researchgate.net/publication/288776928_Alternating_two-frequency_countercurrent_ultrasonic-assisted_extraction_of_protein_and_polysaccharide_from_Porphyra_yezoensis https://www.researchgate.net/publication/288776928_Alternating_two-frequency_countercurrent_ultrasonic-assisted_extraction_of_protein_and_polysaccharide_from_Porphyra_yezoensis , .

D A Rees , E Conway . The structure and biosynthesis of porphyran: a comparison of some samples . The Biochemical Journal , 1962 . 84 ( 2 ): 411 - 416 . DOI: 10.1042/bj0840411 http://doi.org/10.1042/bj0840411 .

S L Rodríguez-Ambriz , A L Martínez-Ayala , F Millán , G Dávila-Ortíz . Composition and functional properties of Lupinus campestris protein isolates . Plant Foods for Human Nutrition , 2005 . 60 ( 3 ): 99 - 107 . DOI: 10.1007/s11130-005-6835-z http://doi.org/10.1007/s11130-005-6835-z .

S Rouimi , C Schorsch , C Valentini , S Vaslin . Foam stability and interfacial properties of milk protein-surfactant systems . Food Hydrocolloids , 2005 . 19 ( 3 ): 467 - 478 . DOI: 10.1016/j.foodhyd.2004.10.032 http://doi.org/10.1016/j.foodhyd.2004.10.032 .

Söderberg J. 2013. Functional properties of legume proteins compared to egg proteins and their potential as egg replacers in vegan food. Second cycle, A2E. Uppsala: SLU, Dept. of Food Science.

H H Sun , W J Mao , Y Chen , S D Guo , H Y Li , X H Qi , Y L Chen , J Xu . Isolation, chemical characteristics and antioxidant properties of the polysaccharides from marine fungus Penicillium sp.F23-2 . Carbohydrate Polymers , 2009 . 78 ( 1 ): 117 - 124 . DOI: 10.1016/j.carbpol.2009.04.017 http://doi.org/10.1016/j.carbpol.2009.04.017 .

K Suresh Kumar , K Ganesan , K Selvaraj , P V Subba Rao . Studies on the functional properties of protein concentrate of Kappaphycus alvarezii (Doty) Doty—an edible seaweed . Food Chemistry , 2014 . 153 353 - 360 . DOI: 10.1016/j.foodchem.2013.12.058 http://doi.org/10.1016/j.foodchem.2013.12.058 .

Y P Timilsena , R Adhikari , C J Barrow , B Adhikari . Physicochemical and functional properties of protein isolate produced from Australian chia seeds . Food Chemistry , 2016 . 212 648 - 656 . DOI: 10.1016/j.foodchem.2016.06.017 http://doi.org/10.1016/j.foodchem.2016.06.017 .

B Wathelet . Nutritional analyses for proteins and amino acids in beans ( Phaseolus sp.) . Biotechnology, Agronomy, Society and Environment , 1999 . 3 ( 4 ): 197 - 200 . https://www.researchgate.net/publication/26392425_Nutritional_analyses_for_proteins_and_amino_acids_in_beans_Phaseolus_sp https://www.researchgate.net/publication/26392425_Nutritional_analyses_for_proteins_and_amino_acids_in_beans_Phaseolus_sp , .

S Y Xu , X Q Chen , Y Liu , K L Cheong . Ultrasonic/microwave-assisted extraction, simulated digestion, and fermentation in vitro by human intestinal flora of polysaccharides from Porphyra haitanensis . International Journal of Biological Macromolecules , 2020 . 152 748 - 756 . DOI: 10.1016/j.ijbiomac.2020.02.305 http://doi.org/10.1016/j.ijbiomac.2020.02.305 .

W Yaphe . Colorimetric determination of 3, 6-anhydrogalactose and galactose in marine algal polysaccharides . Analytical Chemistry , 1960 . 32 ( 10 ): 1327 - 1330 . DOI: 10.1021/ac60166a030 http://doi.org/10.1021/ac60166a030 .

Y P Zhu , X Y Zhao , X W Zhang , H K Liu . Extraction, structural and functional properties of Haematococcus pluvialis protein after pigment removal . International Journal of Biological Macromolecules , 2019 . 140 1073 - 1083 . DOI: 10.1016/j.ijbiomac.2019.08.209 http://doi.org/10.1016/j.ijbiomac.2019.08.209 .

Views

Downloads

CSCD

Alert me when the article has been cited

Submit

Tools

Publicity Resources

Provenance of colorless and brown volcanic glass in late Pleistocene tephra layers in the Western Philippine Sea

Cytological and transcriptional analysis reveal phosphatidylinositol signaling pathway plays key role in mitotic division of Pyropia yezoensis

Development of organelle single nucleotide polymorphism (SNP) markers and their application for the identification of cytoplasmic inheritance patterns inPyropia yezoensis(Bangiales, Rhodophyta)

Construction of high-density genetic linkage map of Pyropia yezoensis (Bangiales, Rhodophyta) and identification of red color trait QTLs in the thalli

Defensive physiological characters of Pyropia yezoensis resistant lines to the red rot disease

Related Author

Tiegang LI

Congying LI

Zhifang XIONG

Xiaojing ZHOU

Peng HUANG

Guoliang ZHANG

Zhaohui ZHANG

Zhishun ZHANG

Related Institution

Key Laboratory of Marine Sedimentology and Environmental Geology, First Institute of Oceanography, Ministry of Natural Resources

College of Marine Technology and Environment, Dalian Ocean University

Institute of Marine Chemistry and Environment, Ocean College, Zhejiang University

University of Chinese Academy of Sciences

Laboratory for Marine Geology, Qingdao Marine Science and Technology Center

Address：Editorial Office of JOL, 88 Haijun Rd. Qingdao, 266000, China
Tel：+86-532-82898754 Email：jol@qdio.ac.cn
Technical support is provided by Beijing Founder electronics co., LTD 京ICP备09064830号-19 京公网安备11010802024621
It is recommended to read the content of this site in Chrome&IE9+. Please switch to extreme mode in browser 360.
Cookies We use cookies to help provide and enhance our service and tailor content. By continuing, you agree to the use of cookies.

⁰

Characterization, functional properties, and antioxidant activities of macromolecular extracts isolated from Pyropia yezoensis

DOI：10.1007/s00343-021-0456-8

摘要

Abstract

关键词

Keywords

references