The phycocyanin-chlorophyll-protein complexes isolated from <italic style="font-style: italic">Chroomonas placoidea</italic>

Min CHEN; Qin LI; Yuxuan WANG; Jing WANG; Kun ZHANG

doi:10.1007/s00343-021-0451-0

Your Location：

Home >

Browse articles >

The phycocyanin-chlorophyll-protein complexes isolated from Chroomonas placoidea

Biology | Updated：2023-05-20

- The phycocyanin-chlorophyll-protein complexes isolated from Chroomonas placoidea
- Journal of Oceanology and Limnology Vol. 40, Issue 2, Pages: 690-702(2022)
- Affiliations：
  
  Institute of Life Science, Yantai University, Yantai 264005, China
- Author bio：
  
  Min CHEN, E-mail: chenmclm@163.com
- Funds：
- DOI：10.1007/s00343-021-0451-0
  CLC：
- Received：14 December 2020，
  
  Accepted：27 February 2021，
  
  Online First：22 March 2021，
  
  Published：2022-03
- Accepted：
Scan QR Code
Min CHEN, Qin LI, Yuxuan WANG, et al. The phycocyanin-chlorophyll-protein complexes isolated from Chroomonas placoidea[J]. Journal of Oceanology and Limnology, 2022, 40(2): 690-702.
DOI：

Min CHEN, Qin LI, Yuxuan WANG, et al. The phycocyanin-chlorophyll-protein complexes isolated from Chroomonas placoidea[J]. Journal of Oceanology and Limnology, 2022, 40(2): 690-702. DOI： 10.1007/s00343-021-0451-0.

摘要

Abstract

An active photosystem (PS) Ⅱ particle and two light-harvesting complexes

as well as their sub-complexes that have not been reported previously

were isolated from a cryptophyte

Chroomonas placoidea

by Triton X-100 sucrose density gradient centrifugation. The fluorescence spectra revealed that there were efficient energy couplings between phycocyanin (PC645) and chlorophyll (Chl) within both zones Ⅲ and Ⅳ of the gradient

which were designated respectively as light-harvesting complex and PSⅡ particles whose size was 15-20 nm according to negative staining in electron microscopy. When the two complexes were further resolved into sub-complexes

the energy coupling was retained in the core PSⅡ complex (named as zone Ⅳ-2 of the sucrose gradient)

which contained almost no outer antenna pigment Chl c. Sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) showed that the PC645 components appeared in Chl-containing protein complexes were mainly the β subunit with molecular weight of 20 kDa. These results demonstrate that PC645 in this cryptophyte was structurally but preferentially combined with the light-harvesting complex and PSⅡ core. The excitation energy absorbed by PC645 could be directly transferred to Chl

(especially the long wavelength of Chl

) in the PSⅡ reaction center or via the Chl

-protein complex. The β subunit corresponded to the terminal fluorescence emission and might play an important role in transmitting energy from PC645 to the Chl-protein complex. The results will help in elucidating the architecture and function of the energy transfer system comprising phycobiliproteins and Chl-protein complexes in cryptophytes.

关键词

Keywords

references

Arpin P C, Turner D B, McClure S D, Jumper C C, Mirkovic T, Challa J R, Lee J, Teng C Y, Green B R, Wilk K E, Curmi P M G, Hoef-Emden K, McCamant D W, Scholes G D. 2015. Spectroscopic studies of cryptophyte light harvesting proteins: vibrations and coherent oscillations. The Journal of Physical Chemistry B , 119 (31): 10 025–10 034, https://doi.org/10.1021/acs.jpcb.5b04704..

Bathke L, Rhiel E, Krumbein W E, Marquardt J. 1999. Biochemical and Immunochemical investigations on the light-harvesting system of the cryptophyte Rhodomonas sp.: evidence for a photosystem I specific antenna. Plant Biology , 1 (5): 516–523, https://doi.org/10.1111/j.1438-8677.1999.tb00777.x..

Becker M, Stubbs M T, Huber R. 1998. Crystallization of phycoerythrin 545 of Rhodomonas lens using detergents and unusual additives. Protein Science , 7 (3): 580–586, https://doi.org/10.1002/pro.5560070306..

Chen M, Li S H, Sun L. 2007. A novel phycocyamn-Chla/ c 2 -protein complex isolated from chloroplasts of Chroomonas placoidea . Chinese Chemical Letters , 18 (11): 1 374–1 378, https://doi.org/10.1016/j.cclet.2007.09.025..

Cheregi O, Kotabová E, Prášil O, Schröder W P, Kaňa R, Funk C. 2015. Presence of state transitions in the cryptophyte alga Guillardia theta . Journal of Experimental Botany , 66 (20): 6 461–6 470, https://doi.org/10.1093/jxb/erv362..

Collini E, Wong C Y, Wilk K E, Curmi P M G, Brumer P, Scholes G D. 2010. Coherently wired light-harvesting in photosynthetic marine algae at ambient temperature. Nature , 463 (7281): 644–647, https://doi.org/10.1038/nature08811..

Douglas S E, Penny S L. 1999. The plastid genome of the cryptophyte alga, Guillardia theta : complete sequence and conserved synteny groups confirm its common ancestry with red algae. Journal of Molecular Evolution , 48 (2): 236–244, https://doi.org/10.1007/pl00006462..

Doust A B, Marai C N J, Harrop S J, Wilk K E, Curmi P M G, Scholes G D. 2004. Developing a structure-function model for the cryptophyte phycoerythrin 545 using ultrahigh resolution crystallography and ultrafast laser spectroscopy. Journal of Molecular Biology , 344 (1): 135–153, https://doi.org/10.1016/j.jmb.2004.09.044..

Doust A B, Wilk K E, Curmi P M G, Scholes G D. 2006. The photophysics of cryptophyte light-harvesting. Journal of Photochemistry and Photobiology A: Chemistry , 184 (1–2): 1–17, https://doi.org/10.1016/jophotochem.2006.06.006..

Gantt E, Edwards M R, Provasoli L. 1971. Chloroplast structure of the Cryptophyceae: evidence for phycobiliproteins within intrathylakoidal spaces. Journal of Cell Biology , 48 (2): 280–290, https://doi.org/10.1083/jcb.48.2.280..

Greenwold M J, Cunningham B R, Lachenmyer E M, Pullman J M, Richardson T L, Dudycha J L. 2019. Diversification of light capture ability was accompanied by the evolution of phycobiliproteins in cryptophyte algae. Proceedings of the Royal Society B: Biological Sciences , 286 (1902): 20190655, https://doi.org/10.1098/rspb.2019.0655..

Harrop S J, Wilk K E, Dinshaw R, Collini E, Mirkovic T, Teng C Y, Oblinsky D G, Green B R, Hoef-Emden K, Hiller R G, Scholes G D, Curmi P M G. 2014. Single-residue insertion switches the quaternary structure and exciton states of cryptophyte light-harvesting proteins. Proceedings of the National Academy of Sciences of the United States of America , 111 (26): E2 666-E2 675, https://doi.org/10.1073/pnas.1402538111..

Ingram K, Hiller R G. 1983. Isolation and characterization of a major chlorophyll ac 2 light-harvesting protein from a Chroomonas species (Cryptophyceae). Biochimica et Biophysica Acta (BBA) — Bioenergetics , 722 (2): 310–319, https://doi.org/10.1016/0005-2728(83)90078-6..

Janssen J, Rhiel E. 2008. Evidence of monomeric photosystem I complexes and phosphorylation of chlorophyll a/c -binding polypeptides in Chroomonas sp. strain LT (Cryptophyceae). International Microbiology, the Official Journal of the Spanish Society for Microbiology , 11 (3): 171–178..

Jeffrey S W, Humphrey G F. 1975. New spectrophotometric equations for determining chlorophylls a, b, c 1 and c 2 in higher plants, algae and natural phytoplankton. Biochemie und Physiologie der Pflanzen , 167 (2): 191–194, https://doi.org/10.1016/S0015-3796(17)30778-3..

Kieselbach T, Cheregi O, Green B R, Funk C. 2018. Proteomic analysis of the phycobiliprotein antenna of the cryptophyte alga Guillardia theta cultured under different light intensities. Photosynthesis Research , 135 (1): 149–163, https://doi.org/10.1007/s11120-017-0400-0..

Laemmli U K. 1970. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature , 227 (5259): 680–685, https://doi.org/10.1038/227680a0..

Li W J, Chen M. 2013. Structural and functional stability of phycocyanin from Chroomonas placoidea . Marine Sciences , 37 (7): 33–40. (in Chinese with English abstract).

Lichtlé C, Duval J C, Lemoine Y 1987. Comparative biochemical, functional and ultrastructural studies of photosystem particles from a Cryptophycea: cryptomonas rufescens ; isolation of an active phycoerythrin particle. Biochimica et Biophysica Acta (BBA) — Bioenergetics , 894 (1): 76–90, https://doi.org/10.1016/0005-2728(87)90214-3..

Lichtlé C, Jupin H, Duval J C. 1980. Energy transfers from Photosystem I to Photosystem I in Cryptomonas rufescens (Cryptophyceae). Biochimica et Biophysica Acta ( BBA ) — Bioenergetics , 591 (1): 104–112, https://doi.org/10.1016/0005-2728(80)90224-8..

Ludwig M, Gibbs S P. 1989. Localization of phycoerythrin at the lumenal surface of the thylakoid membrane in Rhodomonas lens . Journal of Cell Biology , 108 (3): 875–884, https://doi.org/10.1083/jcb.108.3.875..

MacColl R, Malak H, Cipollo J, Label B, Ricci G, MacColl D, Eisele L E. 1995. Studies on the dissociation of cryptomonad biliproteins. Journal of Biological Chemistry , 270 (46): 27 555–27 561, https://doi.org/10.1074/jbc.270.46.27555..

MacColl R, Malak H, Gryczynski I, Eisele L E, Mizejewski G J, Franklin E, Sheikh H, Montellese D, Hopkins S, MacColl L C. 1998. Phycoerythrin 545: monomers, energy migration, bilin topography, and monomer/dimer equilibrium. Biochemistry , 37 (1): 417–423, https://doi.org/10.1021/bi971453s..

Marin A, Doust A B, Scholes G D, Wilk K E, Curmi P M G, van Stokkum I H M, van Grondelle R. 2011. Flow of excitation energy in the cryptophyte light-harvesting antenna phycocyanin 645. Biophysical Journal , 101 (4): 1 004–1 013, https://doi.org/10.1016/j.bpj.2011.07.012..

Mirkovic T, Doust A B, Kim J, Wilk K E, Curutchet C, Mennucci B, Cammi R, Curmi P M G, Scholes G D. 2007. Ultrafast light harvesting dynamics in the cryptophyte phycocyanin 645. Photochemical & Photobiological Sciences: Official Journal of the European Photochemistry Association and the European Society for Photobiology , 6 (9): 964–975, https://doi.org/10.1039/b704962e..

Mirkovic T, Ostroumov E E, Anna J M, van Grondelle R, Govindjee, Scholes G D. 2017. Light absorption and energy transfer in the antenna complexes of photosynthetic organisms. Chemical Reviews , 117 (2): 249–293, https://doi.org/10.1021/acs.chemrev.6b00002..

Morisset W, Wehrmeyer W, Schirmer T, Bode W. 1984. Crystallization and preliminary x-ray diffraction data of the cryptomonad biliprotein phycocyanin-645 from a Chroomonas spec. Archives of Microbiology , 140 (2): 202–205, https://doi.org/10.1007/BF00454927..

Novoderezhkin V I, Doust A B, Curutchet C, Scholes G D. 2010. Excitation dynamics in phycoerythrin 545: modeling of steady-state spectra and transient absorption with modified Redfield theory. Biophysical Journal , 99 (2): 344–352, https://doi.org/10.1016/j.bpj.2010.04.039..

Overkamp K E, Langklotz S, Aras M, Helling S, Marcus K, Bandow J E, Hoef-Emden K, Frankenberg-Dinkel N. 2014. Chromophore composition of the phycobiliprotein Cr-PC577 from the cryptophyte Hemiselmis pacifica . Photosynthesis Research , 122 (3): 293–304, https://doi.org/10.1007/s11120-014-0029-1..

Samuelsson G, Prezélin B B. 1985. Photosynthetic electron transport in cell-free extracts of diverse phytoplankton. Journal of Phycology , 21 : 453–457, https://doi.org/10.1111/j.0022-3646.1985.00453.x.

Spear-Bernstein L, Miller K R. 1987. Immunogold localization of the phycobiliprotein of a cryptophyte alga to the intrathylakoidal space. In : Biggins J ed. Progress in Photosynthesis Research. Martinus Nijhoff Publishing, the Netherlands. p. 309–312.

Teaching and Research Group of Plant Physiology, Department of Biology, East China Normal University. 1980. Guidance of Plant Physiological Experiment. People's Education Publication, Beijing. p. 116–117. (in Chinese)

Tomazic N, Overkamp K E, Aras M, Pierik A J, Hofmann E, Frankenberg-Dinkel N. 2020. Exchange of a single amino acid residue in the cryptophyte phycobiliprotein lyase Gt CPES expands its substrate specificity. BioRxiv , https://doi.org/10.1101/2020.03.31.018853..

van der Weij-De Wit C D, Doust A B, van Stokkum I H M, Dekker J P, Wilk K E, Curmi P M G, Scholes G D, van Grondelle R. 2006. How energy funnels from the phycoerythrin antenna complex to photosystem I and photosystem Ⅱ in Cryptophyte Rhodomonas CS24 Cells. The Journal of Physical Chemistry B , 110 (49): 25 066–25 073, https://doi.org/10.1021/jp061546w..

Wedemayer G J, Kidd D G, Glazer A N. 1996. Cryptomonad biliproteins: bilin types and locations. Photosynthesis Research , 48 (1): 163–170, https://doi.org/10.1007/BF00041006..

Wei X P, Su X D, Cao P, Liu X Y, Chang W R, Zhang X Z, Liu Z F. 2016. Structure of spinach photosystem Ⅱ-LHCⅡ supercomplex at 3.2 Å resolution. Nature , 534 (7605): 69–74, https://doi.org/10.1038/nature18020..

Wilk K E, Harrop S J, Jankova L, Edler D, Keenan G, Sharples F, Hiller R G, Curmi P M G. 1999. Evolution of a light-harvesting protein by addition of new subunits and rearrangement of conserved elements: crystal structure of a Cryptophyte phycoerythrin at 1.63-Å resolution. Proceedings of the National Academy of Sciences of The United States of America , 96 (16): 8 901–8 906, https://doi.org/10.1073/pnas.96.16.8901..

Zhao L S, Huokko T, Wilson S, Simpson D M, Wang Q, Ruban A V, Mullineaux C W, Zhang Y Z X, Liu L N. 2020. Structural variability, coordination and adaptation of a native photosynthetic machinery. Nature Plants , 6 (7): 869–882, https://doi.org/10.1038/s41477-020-0694-3..

Views

Downloads

CSCD

Alert me when the article has been cited

Submit

Tools

Publicity Resources

Effect of high light and desiccation on photosystem Ⅱ in the seedlings and mature plants of tropical seagrass Enhalus acoroides during low tide

Effects of the heavy metal cadmium on photosynthetic activity and the xanthophyll cycle in Phaeodactylum tricornutum

Numerical Study of High Bypass Ratio Fan/BoosterStage with Inlet Distortion

Study on Temperature Field Characteristics of2.5D Braided Composites

Investigation on Interaction Mechanism Between Rim Sealing Flow and Mainstream Flow in High-Pressure Turbine

Related Author

Xingkai CHE

Hu LI

Litao ZHANG

Jianguo LIU

Yan JI

Xiujun XIE

Guangce WANG

Related Institution

CAS and Shandong Key Laboratory of Experimental Marine Biology, Center for Ocean Mega-Science, Institute of Oceanology, Chinese Academy of Sciences

Laboratory for Marine Biology and Biotechnology, Pilot National Laboratory for Marine Science and Technology (Qingdao)

School of Biological and Chemical Engineering, Qingdao Technical College

Key Laboratory of Experimental Marine Biology, Institute of Oceanology, Chinese Academy of Sciences

Laboratory for Marine Biology and Biotechnology, Qingdao National Laboratory for Marine Science and Technology

Address：Editorial Office of JOL, 88 Haijun Rd. Qingdao, 266000, China
Tel：+86-532-82898754 Email：jol@qdio.ac.cn
Technical support is provided by Beijing Founder electronics co., LTD 京ICP备09064830号-19 京公网安备11010802024621
It is recommended to read the content of this site in Chrome&IE9+. Please switch to extreme mode in browser 360.
Cookies We use cookies to help provide and enhance our service and tailor content. By continuing, you agree to the use of cookies.

⁰

The phycocyanin-chlorophyll-protein complexes isolated from Chroomonas placoidea

DOI：10.1007/s00343-021-0451-0

摘要

Abstract

关键词

Keywords

references