Biocompatibility of marine magnetotactic ovoid strain MO-1 for in vivo application

Changyou CHEN; Pingping WANG; Long-Fei WU; Tao SONG

doi:10.1007/s00343-021-0420-7

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Biocompatibility of marine magnetotactic ovoid strain MO-1 for in vivo application

Special Section: Magnetotactic Bacteria and Magnetoreception | Updated：2023-05-20

- Biocompatibility of marine magnetotactic ovoid strain MO-1 for in vivo application
- Journal of Oceanology and Limnology Vol. 39, Issue 6, Pages: 2107-2115(2021)
- Affiliations：
  
  1.Beijing Key Laboratory of Bioelectromagnetism, Institute of Electrical Engineering, Chinese Academy of Sciences, Beijing 100190, China
  2.France-China Joint Laboratory for Evolution and Development of Magnetotactic Multicellular Organisms, Chinese Academy of Sciences, Beijing 100029, China
  3.Aix Marseille University, CNRS, LCB, Marseille F-13402, France
  4.University of the Chinese Academy of Sciences, Beijing 100049, China
- Author bio：
  
  Pingping WANG, wangpp@mail.iee.ac.cn
- Funds：
- DOI：10.1007/s00343-021-0420-7
  CLC：
- Received：29 October 2020，
  
  Accepted：29 November 2020，
  
  Online First：01 April 2021，
  
  Published：2021-11
- Accepted：
Scan QR Code
Changyou CHEN, Pingping WANG, Long-Fei WU, et al. Biocompatibility of marine magnetotactic ovoid strain MO-1 for in vivo application[J]. Journal of Oceanology and Limnology, 2021, 39(6): 2107-2115.
DOI：

Changyou CHEN, Pingping WANG, Long-Fei WU, et al. Biocompatibility of marine magnetotactic ovoid strain MO-1 for in vivo application[J]. Journal of Oceanology and Limnology, 2021, 39(6): 2107-2115. DOI： 10.1007/s00343-021-0420-7.

摘要

Abstract

Magnetotactic bacteria are capable of biosynthesizing magnetic nanoparticles

also called magnetosomes

and swimming along magnetic field lines. The abilities endow the whole cells of magnetotactic bacteria with such applications as targeted therapy and manipulation of microrobots. We have shown that the intact marine magnetotactic bacteria MO-1 kill efficiently antibiotic-resistant pathogen

Staphylococcus aureus

in vivo

but the biocompatibility of this marine bacterium is unknown. In this study

the strain MO-1 was chosen to analyze its biocompatibility and potential for biomedicine applications. Results showed that MO-1 cells could be guided at 37 ℃ under an external magnetic field and swim in the blood plasma and urine. They could keep active locomotivity within 40 min in the plasma and urine

although their velocity slowed down. When incubated with human cells

magnetotactic bacteria MO-1 had no obvious effects on cellular viability at low dose

while the cell toxicity increased with the augmentation of the quantity of the MO-1 cells added. In the in-vivo experiments

the median lethal dose of magnetotactic bacteria MO-1 in rats was determined to be 7.9×10

bacteria/kg. These results provided the foundation for the biocompatibility and safety evaluations of magnetotactic bacteria MO-1 and suggested that they could be basically used in clinical targeted therapy.

关键词

Keywords

references

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