NMR-based metabolomic responses of freshwater gastropod <italic style="font-style: italic">Bellamya aeruginosa</italic> to MC-producing and non MC-producing <italic style="font-style: italic">Microcystis aeruginosa</italic>

YANG Wen; YE Yangfang; LU Kaihong; ZHENG Zhongming; ZHU Jinyong

doi:10.1007/s00343-021-0336-2

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NMR-based metabolomic responses of freshwater gastropod Bellamya aeruginosa to MC-producing and non MC-producing Microcystis aeruginosa

Biology | Updated：2023-04-27

- NMR-based metabolomic responses of freshwater gastropod Bellamya aeruginosa to MC-producing and non MC-producing Microcystis aeruginosa
- Journal of Oceanology and Limnology Vol. 40, Issue 1, Pages: 260-272(2022)
- Affiliations：
  
  School of Marine Science, Ningbo University, Ningbo 315800, China
- Author bio：
  
  Jinyong ZHU, zhujinyong@nbu.edu.cn
- Funds：
- DOI：10.1007/s00343-021-0336-2
  CLC：
- Received：28 August 2020，
  
  Accepted：16 November 2020，
  
  Online First：22 January 2021，
  
  Published：2022-01
- Accepted：
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YANG Wen, YE Yangfang, LU Kaihong, et al. NMR-based metabolomic responses of freshwater gastropod Bellamya aeruginosa to MC-producing and non MC-producing Microcystis aeruginosa[J]. Journal of Oceanology and Limnology, 2022, 40(1): 260-272.
DOI：

YANG Wen, YE Yangfang, LU Kaihong, et al. NMR-based metabolomic responses of freshwater gastropod Bellamya aeruginosa to MC-producing and non MC-producing Microcystis aeruginosa[J]. Journal of Oceanology and Limnology, 2022, 40(1): 260-272. DOI： 10.1007/s00343-021-0336-2.

摘要

Abstract

Molluscan metabolomic analysis is essential for the understanding of the regulatory mechanism of aquatic invertebrate in response to hepatotoxic microcystins (MCs) stress. To understand the system responses of the gastropod to MC exposure

metabolomic alterations caused by two strains (MC-producing and non MC-producing) of

Microcystis aeruginosa

were characterized indifferent biological matrices (hepatopancreas and muscle) of

Bellamya aeruginosa

(Gastropoda) using

H nuclear magnetic resonance (NMR) spectroscopy combined with MCs detections after exposure for 1

and 14 d. Although ELISA analysis showed that no MCs was detected in both tissues after non MC-producing

M. aeruginosa

exposure

MCs concentrations were increasing in the hepatopancreas (from 1.29±0.48 μg/g to 3.17±0.11 μg/g) and foot muscle (from 0.07±0.02 μg/g to 0.21±0.08 μg/g) after 14-d exposure of MC-producing

M. aeruginosa

. Meanwhile

we observed that MC induced significant increase in creatine

a variety of amino acids (leucine

isoleucine

valine

threonine

alanine

methionine

glutamate

aspartate

and lysine)

carboxylic acids (lactate

acetate

and D-3-hydroxybutyrate)

and choline and its derivatives (phosphocholine and glycerophosphocholine) but decreased the energy substance (lipids

glucose

and glycogen) in the hepatopancreas. However

no significant metabolite differences were observed in the muscle between MC-producing and non MC-producing cyanobacteria treated groups. These results suggest that MC exposure may cause hepatic energy expenditure accompanied with various metabolic disorders that involve lipid metabolism

protein catabolism

osmoregulation

glycolysis

glycogenolysis

and tricarboxylic acid (TCA) cycle. Moreover

metabolic perturbation was aggravated as the level of accumulated MCs raised over time in the MC-producing cyanobacteria treatment. These findings indicated that MCs accumulation might lead to oxidative-stress-mediated damage of mitochondria functions.

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Keywords

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NMR-based metabolomic responses of freshwater gastropod Bellamya aeruginosa to MC-producing and non MC-producing Microcystis aeruginosa

DOI：10.1007/s00343-021-0336-2

摘要

Abstract

关键词

Keywords

references