MamZ protein plays an essential role in magnetosome maturation process of <italic style="font-style: italic">Magnetospirillum gryphiswaldense</italic> MSR-1

Sha WU; Qing WANG; Xu WANG; Ruixue GUO; Tongwei ZHANG; Yongxin PAN; Feng LI; Ying LI

doi:10.1007/s00343-021-0321-9

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MamZ protein plays an essential role in magnetosome maturation process of Magnetospirillum gryphiswaldense MSR-1

Special Section: Magnetotactic Bacteria and Magnetoreception | Updated：2023-05-20

- MamZ protein plays an essential role in magnetosome maturation process of Magnetospirillum gryphiswaldense MSR-1
- Journal of Oceanology and Limnology Vol. 39, Issue 6, Pages: 2082-2096(2021)
- Affiliations：
  
  1.Engineering Technology Research Center of Ecological Restoration and Comprehensive Utilization of Coal Mining Collapse Area, Anhui Province; Anhui Province Key Laboratory of Pollutant Sensitive Materials and Environmental Remediation, College of Life Sciences, Huaibei Normal University, Huaibei 235000, China
  2.State Key Laboratories for Agro-biotechnology, China Agricultural University, Beijing 100193, China
  3.Paleomagnetism and Geochronology Laboratory, Key Laboratory of Earth and Planetary Physics, Institute of Geology and Geophysics, Chinese Academy of Sciences, France-China Joint Laboratory for Evolution and Development of Magnetotactic Multicelluar Organisms, Beijing 100029, China
- Author bio：
  
  Feng LI, rx2500@163.com
  Ying LI, yingli528@vip.sina.com
- Funds：
- DOI：10.1007/s00343-021-0321-9
  CLC：
- Received：27 August 2020，
  
  Accepted：29 September 2020，
  
  Online First：28 December 2020，
  
  Published：2021-11
- Accepted：
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Sha WU, Qing WANG, Xu WANG, et al. MamZ protein plays an essential role in magnetosome maturation process of Magnetospirillum gryphiswaldense MSR-1[J]. Journal of Oceanology and Limnology, 2021, 39(6): 2082-2096.
DOI：

Sha WU, Qing WANG, Xu WANG, et al. MamZ protein plays an essential role in magnetosome maturation process of Magnetospirillum gryphiswaldense MSR-1[J]. Journal of Oceanology and Limnology, 2021, 39(6): 2082-2096. DOI： 10.1007/s00343-021-0321-9.

摘要

Abstract

Based on analysis of gene structure of

mamXY

operon in

Magnetospirillum gryphiswaldense

strain MSR-1

we constructed a

mamZ

deletion mutant strain (∆

mamZ

) and four complemented strains with different

mamZ

fragment lengths. Various cell phenotypic and physiological parameters were evaluated and compared among the wild-type (WT)

mutant

and complemented strains. Cell growth rates were not notably different; however

magnetic response (Cmag) and iron uptake ability were significantly lower in ∆

mamZ

. High-resolution transmission electron microscopy (HR-TEM) showed that magnetosomes in ∆

mamZ

were small and irregular

and rock magnetic measurements suggested that they contained immature particles. In comparison to WT of MSR-1

intracellular iron content of ∆

mamZ

and the complemented strains cultured with 20 μmol/L iron source was similar or slightly higher. The complemented strains were unable to synthesize mature or normal amounts of magnetosomes

apparently because of abnormal expression of the transmembrane domain of MamZ protein. Real-time reverse transcription polymerase chain reaction (RTqPCR) analysis showed that relative transcription levels of

mamX

and

ftsZ

genes in ∆

mamZ

were higher at 18 h than at 12 h

suggesting that MamXY proteins play cooperative functional roles in the magnetosome maturation process. Transcription level of

mms6

was significantly upregulated in ∆

mamZ

(incubated at 12 h) and the complemented strains (incubated at 12 and 18 h)

reflecting possible interaction between MamXY and Mms6 proteins during magnetosome biosynthesis. These findings

taken together

demonstrate the essential role of MamZ in the magnetosome maturation process in MSR-1.

关键词

Keywords

references

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MamZ protein plays an essential role in magnetosome maturation process of Magnetospirillum gryphiswaldense MSR-1

DOI：10.1007/s00343-021-0321-9

摘要

Abstract

关键词

Keywords

references