Transcriptome profile of <italic style="font-style: italic">Dunaliella salina</italic> in Yuncheng Salt Lake reveals salt-stress-related genes under different salinity stresses

Fan GAO; Fangru NAN; Jia FENG; Junping LÜ; Qi LIU; Xudong LIU; Shulian XIE

doi:10.1007/s00343-021-0164-4

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Transcriptome profile of Dunaliella salina in Yuncheng Salt Lake reveals salt-stress-related genes under different salinity stresses

Biology | Updated：2023-05-20

- Transcriptome profile of Dunaliella salina in Yuncheng Salt Lake reveals salt-stress-related genes under different salinity stresses
- Journal of Oceanology and Limnology Vol. 39, Issue 6, Pages: 2336-2362(2021)
- Affiliations：
  
  School of Life Science, Shanxi Key Laboratory for Research and Development of Regional Plants, Shanxi University, Taiyuan 030006, China
- Author bio：
  
  Shulian XIE, xiesl@sxu.edu.cn
- Funds：
- DOI：10.1007/s00343-021-0164-4
  CLC：
- Received：18 April 2020，
  
  Accepted：08 June 2020，
  
  Online First：28 December 2020，
  
  Published：2021-11
- Accepted：
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Fan GAO, Fangru NAN, Jia FENG, et al. Transcriptome profile of Dunaliella salina in Yuncheng Salt Lake reveals salt-stress-related genes under different salinity stresses[J]. Journal of Oceanology and Limnology, 2021, 39(6): 2336-2362.
DOI：

Fan GAO, Fangru NAN, Jia FENG, et al. Transcriptome profile of Dunaliella salina in Yuncheng Salt Lake reveals salt-stress-related genes under different salinity stresses[J]. Journal of Oceanology and Limnology, 2021, 39(6): 2336-2362. DOI： 10.1007/s00343-021-0164-4.

摘要

Abstract

Salt stress is an abiotic stress to plants in especially saline lakes.

Dunaliella

a halophilic microalga distributed throughout salt lakes and seas

can respond to different salinity stresses by regulating the expression of some genes. However

these genes and their function and biological processes involved remain unclear. Profiling these salt-stress-related genes in a high-salt-tolerant

Dunaliella

species will help clarify the salt tolerance machinery of

Dunaliella

. Three

salina

_YC salt-stress groups were tested under low (0.51 mol/L)

moderate (1.03 mol/L)

and high (3.42 mol/L) NaCl concentrations and one control group under very low (0.05 mol/L) NaCl concentration and 3 transcriptome results that were deep sequenced and de novo assembled were obtained per group. Twelve high-quality RNA-seq libraries with 46 585 upregulated and 47 805 downregulated unigenes were found. Relative to the control

188 common differentially expressed genes (DEGs) were screened and divided into four clusters in expression pattern. Fifteen of them annotated in the significant enriched Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) were validated via qPCR. Their qPCR-based relative expression patterns were similar to their RNA-seq-based patterns. Two significant DEGs

the geranylgeranyl diphosphate synthase coding gene (1 876-bp cDNA) and diacylglycerol O-acyltransferase coding gene (2 968-bp cDNA)

were cloned and analyzed in silico. The total lipid content

superoxide dismutase specific activity

and betacarotene content of

salina

_YC increased gradually with increasing salinity. In addition

the expression of 11 validated genes involved in fatty acid biosynthesis/degradation

active oxygen or carotenoid metabolisms showed significant changes. In addition

algal photochemical efficiency was diminished with increasing salinity

as well as the expression of 4 photosynthesis-related genes. These results could help clarify the molecular mechanisms underlying

salina

responses to the Yuncheng Salt Lake environment and lay a foundation for further utilization of this algal resource.

关键词

Keywords

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Transcriptome profile of Dunaliella salina in Yuncheng Salt Lake reveals salt-stress-related genes under different salinity stresses

DOI：10.1007/s00343-021-0164-4

摘要

Abstract

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