Development of high-resolution chloroplast markers for intraspecific phylogeographic studies of <italic style="font-style: italic">Phaeocystis globosa</italic>

Qingchun ZHANG; Zhuang NIU; Jinxiu WANG; Chao LIU; Fanzhou KONG; Xiaokun HU; Jiayu ZHAO; Rencheng YU

doi:10.1007/s00343-020-9304-5

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Development of high-resolution chloroplast markers for intraspecific phylogeographic studies of Phaeocystis globosa

Ecology | Updated：2023-06-06

- Development of high-resolution chloroplast markers for intraspecific phylogeographic studies of Phaeocystis globosa
- Journal of Oceanology and Limnology Vol. 39, Issue 2, Pages: 508-524(2021)
- Affiliations：
  
  1.CAS Key Laboratory of Marine Ecology and Environmental Sciences, Institute of Oceanology, Chinese Academy of Sciences, Qingdao 266071, China
  2.Laboratory for Marine Ecology and Environmental Sciences, Pilot National Laboratory for Marine Science and Technology (Qingdao), Qingdao 266237, China
  3.Center of Ocean Mega-Science, Chinese Academy of Sciences, Qingdao 266071, China
  4.University of Chinese Academy of Sciences, Beijing 100049, China
- Author bio：
  
  Rencheng YU, E-mail: rcyu@qdio.ac.cn
- Funds：
- DOI：10.1007/s00343-020-9304-5
  CLC：
- Received：18 November 2019，
  
  Accepted：23 January 2020，
  
  Online First：02 March 2020，
  
  Published：2021-02
- Accepted：
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Qingchun ZHANG, Zhuang NIU, Jinxiu WANG, et al. Development of high-resolution chloroplast markers for intraspecific phylogeographic studies of Phaeocystis globosa[J]. Journal of Oceanology and Limnology, 2021, 39(2): 508-524.
DOI：

Qingchun ZHANG, Zhuang NIU, Jinxiu WANG, et al. Development of high-resolution chloroplast markers for intraspecific phylogeographic studies of Phaeocystis globosa[J]. Journal of Oceanology and Limnology, 2021, 39(2): 508-524. DOI： 10.1007/s00343-020-9304-5.

摘要

Abstract

Phaeocystis globosa

is an important harmful algal bloom causative species distributing widely in temperate and tropical coastal waters in the world. The morphological

physiological

and biochemical characteristics are different among geographic strains

which can not be distinguished with nuclear ribosomal DNA markers at present. Therefore

the genetic distance and phylogeographic relationships of nuclear 28S rDNA D1-D2 and ITS regions

and three chloroplast intergenic spacers (

pet

N-t

trn

M1-

psb

and

rbc

S-

rpl

27) were analyzed and compared among 13 strains of

P. globosa

isolated from the Pacific Ocean and Atlantic Ocean in this study. In addition

the nucleotide polymorphisms of 28S rDNA D1-D2

ITS

and

rbc

S-

rpl

27 regions were evaluated in two

P. globosa

strains. The various levels of nucleotide polymorphism were in the nuclear 28S rDNA D1-D2 region and ITS region

but no polymorphism was in the chloroplast

rbc

S-

rpl

27 intergenic spacer. A reasonable intraspecific phylogeographic relationship was presented by

rbc

S-

rpl

27 intergenic spacer

which had the strongest distinction to geographic strains compared to those of 28S rDNA D1-D2 and ITS regions. In the phylogenetic tree of

rbc

S-

rpl

27 intergenic spacer

the two strains from the North Sea of the Atlantic Ocean were divided firstly from the species of

P. globosa

and then formed an independent clade

while the other Atlantic strains and all of Pacific strains joined up to build the other clade. It was implied that at least two genetically distant populations of

P. globosa

existed in the Atlantic coastal regions. This study provided a high-resolution chloroplast marker to analyze intraspecific phylogeographic populations of

P. globosa

and preliminarily clarified the genetic relationships of the Pacific and Atlantic strains of

P. globosa

关键词

Keywords

references

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Related Author

Weinan GUO

Fangru NAN

Zhongshi HE

Xudong LIU

Yang LIU

Qi LIU

Jia FENG

Shulian XIE

Related Institution

School of Life Science, Shanxi Key Laboratory for Research and Development of Regional Plants, Shanxi University

Institute of Marine and Environmental Technology, University of Maryland Center for Environmental Science, Baltimore

School of Life Sciences, Jianghan University

Changjiang Basin Ecology and Environment Monitoring and Scientific Research Center, Changjiang Basin Ecology and Environment Administration, Ministry of Ecology and Environment

Key Laboratory of Algal Biology, Institute of Hydrobiology, Chinese Academy of Sciences

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Development of high-resolution chloroplast markers for intraspecific phylogeographic studies of Phaeocystis globosa

DOI：10.1007/s00343-020-9304-5

摘要

Abstract

关键词

Keywords

references