Comparative transcriptome analysis of abalone <italic style="font-style: italic">Haliotis discus hannai</italic> with green and gray egg colors

Yanwei FENG; Bo YUAN; Xu JIANG; Xiangquan LIU; Zan LI; Guohua SUN; Xiaohui XU

doi:10.1007/s00343-020-9302-7

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Comparative transcriptome analysis of abalone Haliotis discus hannai with green and gray egg colors

Aquaculture and Fisheries | Updated：2023-05-20

- Comparative transcriptome analysis of abalone Haliotis discus hannai with green and gray egg colors
- Journal of Oceanology and Limnology Vol. 39, Issue 1, Pages: 350-361(2021)
- Affiliations：
  
  1.School of Agriculture, Ludong University, Yantai 264025, China
  2.Shandong Provincial Key Laboratory of Restoration for Marine Ecology, Shandong Marine Resource and Environment Research Institute, Yantai 264006, China
  3.National Demonstration Center for Experimental Fisheries Science Education, Shanghai Ocean University, Shanghai 201306, China
  4.Shanghai Engineering Research Center of Aquaculture, Shanghai Ocean University, Shanghai 201306, China
  5.Shandong Rice Research Institute, Shandong Academy of Agricultural Sciences, Jinan 250100, China
- Author bio：
  
  FENG Yanwei, E-mail:fywzxm1228@163.com
  LIU Xiangquan, E-mail:lxq6808@163.com
- Funds：
- DOI：10.1007/s00343-020-9302-7
  CLC：
- Received：06 December 2019，
  
  Accepted：26 January 2020，
  
  Online First：01 April 2020，
  
  Published：2021-01
- Accepted：
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Yanwei FENG, Bo YUAN, Xu JIANG, et al. Comparative transcriptome analysis of abalone Haliotis discus hannai with green and gray egg colors[J]. Journal of Oceanology and Limnology, 2021, 39(1): 350-361.
DOI：

Yanwei FENG, Bo YUAN, Xu JIANG, et al. Comparative transcriptome analysis of abalone Haliotis discus hannai with green and gray egg colors[J]. Journal of Oceanology and Limnology, 2021, 39(1): 350-361. DOI： 10.1007/s00343-020-9302-7.

摘要

Abstract

Haliotis discus hannai

is an important marine economic species in China. Its egg color was found to be associated with economic traits

which provides a new idea for breeding. However

the molecular mechanism of the egg-color formation has not been reported. Thus

the pigment composition and comparative transcriptome analyses of

H. discus hannai

with green and gray egg color were conducted using high-performance liquid chromatography (HPLC) and RNA-Seq methods. Results show that individuals with green and gray eggs both possess the fucoxanthin. Lutein existed in gray-egged individuals

but not in green-egged individuals. In transcriptome analysis

272 310 unigenes were received from 461 162 transcripts with a mean length of 985 bp and N50 of 1 524 bp

respectively. A total of 185 unigenes were identified as differentially expressed genes (DEGs). The DEGs involved in "flavin-containing compound metabolic process"

"melanosome"

"glutathione metabolism"

and "cytochrome b6f complex" were likely related to the formation of the egg color. Our results provide foundational information for the functional analysis of egg-color related genes and are beneficial to the selective breeding of

H. discus hannai

关键词

Keywords

references

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Comparative transcriptome analysis of abalone Haliotis discus hannai with green and gray egg colors

DOI：10.1007/s00343-020-9302-7

摘要

Abstract

关键词

Keywords

references