Ultrastructural variation and key ER chaperones response induced by heat stress in intestinal cells of sea cucumber <italic style="font-style: italic">Apostichopus japonicus</italic>

Shasha WANG; Yingqiu ZHENG; Muyan CHEN; Kenneth B. STOREY

doi:10.1007/s00343-020-9265-8

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Ultrastructural variation and key ER chaperones response induced by heat stress in intestinal cells of sea cucumber Apostichopus japonicus

Aquaculture and Fisheries | Updated：2023-05-20

- Ultrastructural variation and key ER chaperones response induced by heat stress in intestinal cells of sea cucumber Apostichopus japonicus
- Journal of Oceanology and Limnology Vol. 39, Issue 1, Pages: 317-328(2021)
- Affiliations：
  
  1.Key Laboratory of Mariculture (Ocean University of China), Ministry of Education, Qingdao 266003, China
  2.Institute of Biochemistry, Carleton University, Ottawa ON K1S 5B6, Canada
- Author bio：
  
  CHEN Muyan, E-mail:chenmuyan@ouc.edu.cn
- Funds：
- DOI：10.1007/s00343-020-9265-8
  CLC：
- Received：04 November 2019，
  
  Accepted：19 February 2020，
  
  Online First：14 March 2020，
  
  Published：2021-01
- Accepted：
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Shasha WANG, Yingqiu ZHENG, Muyan CHEN, et al. Ultrastructural variation and key ER chaperones response induced by heat stress in intestinal cells of sea cucumber Apostichopus japonicus[J]. Journal of Oceanology and Limnology, 2021, 39(1): 317-328.
DOI：

Shasha WANG, Yingqiu ZHENG, Muyan CHEN, et al. Ultrastructural variation and key ER chaperones response induced by heat stress in intestinal cells of sea cucumber Apostichopus japonicus[J]. Journal of Oceanology and Limnology, 2021, 39(1): 317-328. DOI： 10.1007/s00343-020-9265-8.

摘要

Abstract

The unfolded protein response (UPR) is an important protective and compensatory strategy used during endoplasmic reticulum stress caused by factors including glucose starvation

low pH

or heat shock. However

there is very little information on the possible role(s) of the UPR under adverse conditions experienced by marine invertebrates. We observed that rough endoplasmic reticulum (ER) was dramatically expanded and numerous autophagosomes were accumulated in the intestinal cells of sea cucumbers

Apostichopus japonicus

under heat stress (4 h at 25℃ compared with 15℃ controls). Moreover

heat stress led to sharp increases in the relative transcript and protein expression levels of two primary ER chaperones:the endoplasmic reticulum resident protein 29-like (ERP29) and protein disulfide-isomerase A6-like (PDIA6). These results suggest a potential adaptive mechanism to deal with heat-induced stress in sea cucumber intestine.

关键词

Keywords

references

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Ultrastructural variation and key ER chaperones response induced by heat stress in intestinal cells of sea cucumber Apostichopus japonicus

DOI：10.1007/s00343-020-9265-8

摘要

Abstract

关键词

Keywords

references