Karyological observations of <italic style="font-style: italic">Ulva linza</italic> chromosomes

Xiaohui ZHAO; Xiaoqian YANG; Jianheng ZHANG; Qinlin WEN; Peimin HE

doi:10.1007/s00343-020-9105-x

Your Location：

Home >

Browse articles >

Karyological observations of Ulva linza chromosomes

Biology | Updated：2023-05-20

- Karyological observations of Ulva linza chromosomes
- Journal of Oceanology and Limnology Vol. 39, Issue 1, Pages: 259-265(2021)
- Affiliations：
  
  1.College of Marine Ecology and Environment, Shanghai Ocean University, Shanghai 201306, China
  2.Water Environment & Engineering Research Center of Shanghai Institution of Higher Education, Shanghai 201306, China
- Author bio：
  
  Jianheng ZHANG, E-mail:jh-zhang@shou.edu.cn
  Peimin HE, E-mail:pmhe@shou.edu.cn
- Funds：
- DOI：10.1007/s00343-020-9105-x
  CLC：
- Received：21 April 2019，
  
  Accepted：23 September 2019，
  
  Online First：16 February 2020，
  
  Published：2021-01
- Accepted：
Scan QR Code
Xiaohui ZHAO, Xiaoqian YANG, Jianheng ZHANG, et al. Karyological observations of Ulva linza chromosomes[J]. Journal of Oceanology and Limnology, 2021, 39(1): 259-265.
DOI：

Xiaohui ZHAO, Xiaoqian YANG, Jianheng ZHANG, et al. Karyological observations of Ulva linza chromosomes[J]. Journal of Oceanology and Limnology, 2021, 39(1): 259-265. DOI： 10.1007/s00343-020-9105-x.

摘要

Abstract

Ulva linza

is one of the species that causes green tides in the Yellow Sea

China. Due to the difficulties in chromosomal preparation

the large numbers of chromosomes

and their relatively small sizes

there have been no reported studies on

Ulva

macroalgae chromosomes. The karyotypes and chromosomes in

U. linza

were observed after a series of treatments. The chromosomes were pretreated with 0.1% colchicine for 12 h and then mixed with enzymes. The samples were dropped from 30 cm height onto glass slides

which spread out the surface coat. These pretreatments were the optimal chromosomes preparation treatments. The prepared chromosomes were stained with 4'

6-diamidino-2-phenylindole (DAPI)

which is a fluorescent probe sensitive and specific to DNA. The chromosome number in the haploid male and female gametophytes was

and was 2

=18 in the diploid sporophytes. The female gametophyte chromosomes were between 0.804 and 2.292 μm in size

the male gametophyte chromosomes were between 0.917 and 2.916 μm

and the sporophyte chromosomes were between 0.912 and 2.167 μm. The relative sizes of the chromosomes were used to analyze the karyotypes of the female and male gametophyte chromosomes. The results provide a solid foundation for the basic technique that can be used to localize molecular markers of

Ulva

chromosomes.

关键词

Keywords

references

Berends P, Merz M, Kranz B, Thaler T, Appel D, Rabe S, Blank I, Stressler T, Fischer L. 2015. Optimization of an enzymatic wheat gluten hydrolysis process in an enzyme membrane reactor using a design of experiment approach. European Food Research and Technology , 242 (10): 1 735-1 746..

Burrell P C. 1939. Root-tip smear method for difficult material. Stain Technology , 14 (4): 147-149..

Chamani E, Tahami S K, Zare N, Asghari-Zakaria R, Mohebodini M, Joyce D. 2012. Effect of different cellulase and pectinase enzyme treatments on protoplast isolation and viability in Lilium ledebeourii bioss . Notulae Botanicae Horti Agrobotanici Cluj - Napoca , 40 (2): 123-128..

Cui J J, Shi J T, Zhang J H, Wang L T, Fan S Y, Xu Z Y, Huo Y Z, Zhou Q Y, Lu Y W, He P M. 2018. Rapid expansion of Ulva blooms in the Yellow Sea, China through sexual reproduction and vegetative growth. Marine Pollution Bulletin , 130 : 223-228..

Dipakkore S, Reddy C R K, Jha B. 2005. Production and seeding of protoplasts of Porphyra okhaensis (Bangiales, Rhodophyta) in laboratory culture. Journal of Applied Phycology , 17 (4): 331-337..

Han W, Chen L P, Zhang J H, Tian X L, Hua L, He Q, Huo Y Z, Yu K F, Shi D J, Ma J H, He P M. 2013. Seasonal variation of dominant free-floating and attached Ulva species in Rudong coastal area, China. Harmful Algae , 28 : 46-54..

Hiraoka M, Dan A, Shimada S, Hagihira M, Migita M, Ohno M. 2003. Different life histories of Enteromorpha prolifera (Ulvales, Chlorophyta) from four rivers on Shikoku Island, Japan. Phycologia , 42 (3): 275-284..

Kennish M J. 2016. Macroalgae. Netherlands: Springer.

Khan T A, Bhise M P, Lakra W S. 2000. Chromosome manipulation in fish-a review. The Indian Journal of Animal Sciences , 70 (2): 213-221..

Kindiger B. 1994. A technique for the preparation of somatic chromosomes of Maize . In : Freeling M, Walbot V eds. The Maize Handbook. Springer, New York..

Lin M S, Comings D E, Alfi O S. 1977. Optical studies of the interaction of 4'-6 diamidino-2-phenylindole with DNA and metaphase chromosomes. Chromosoma , 60 (1): 15-25..

Liu D Y, Keesing J K, He P M, Wang Z L, Shi Y J, Wang Y J. 2013. The world's largest macroalgal bloom in the Yellow Sea, China: formation and implications. Estuarine , Coastal and Shelf Science , 129 : 2-10..

Liu Y, Bi Y H, Gu J G, Li L H, Zhou Z G. 2012b. Localization of a female-specific marker on the chromosomes of the brown seaweed Saccharina japonica using fluorescence in situ hybridization. PLoS One , 7 (11): e48784..

Liu Y, Bi Y H, Zhou Z G. 2012a. Karyological observation on Saccharina japonica chromosomes stained with DAPI. Journal of Fisheries of China , 36 (1): 50-54. (in Chinese with English abstract).

Namboodiri A N. 1973. Colchicine pretreatment and maceration for improved squashes of dipterocarpaceae. Stain Technology , 48 (2): 98-100..

Nichols C. 1941. Spontaneous chromosome aberrations in allium. Genetics , 26 (1): 89-100..

Oliveira L, Bisalputra T, Antia N J. 1980. Ultrastructural observation of the surface coat of Dunaliella tertiolecta from staining with cationic dyes and enzyme treatments. New Phytologist , 85 (3): 385-392..

Peterfi L S, Manton I. 1986. Observations with the electron microscope on Asteromonas gracilis Artari emend. ( Stephanoptera gracilis (Artari) Wisl.), with some comparative observations on Dunaliella sp. British Phycological Bulletin , 3 (3): 423-440..

Raikar S V, Braun R H, Bryant C, Conner A J, Christey M C. 2008. Efficient isolation, culture and regeneration of Lotus corniculatus protoplasts. Plant Biotechnology Reports , 2 (3): 171..

Schweizer D. 1976. Reverse fluorescent chromosome banding with chromomycin and DAPI. Chromosoma , 58 (4): 307-324..

Tan I H, Blomster J, Hansen G, Leskinen E, Maggs C A, Mann D G, Sluiman H J, Stanhope M J. 1999. Molecular phylogenetic evidence for a reversible morphogenetic switch controlling the gross morphology of two common genera of green seaweeds, Ulva and Enteromorpha . Molecular Biology and Evolution , 16 (8): 1 011-1 018..

Teasdale R D, Rugini E. 1983. Preparation of viable protoplasts from suspension-cultured loblolly pine ( Pinus taeda ) cells and subsequent regeneration to callus. Plant Cell , Tissue and Organ Culture , 2 (3): 253-261..

Wang K, Yu W C. 2016. Chromosome preparation in Rice ( Oryza sativa ). Current Protocols in Plant Biology , 1 (1): 67-77..

Wang S Y, Huo Y Z, Zhang J H, Cui J J, Wang Y, Yang L L, Zhou Q Y, Lu Y W, Yu K F, He P M. 2018. Variations of dominant free-floating Ulva species in the source area for the world's largest macroalgal blooms, China: differences of ecological tolerance. Harmful Algae , 74 : 58-66..

White G B. 1973. Comparative studies on sibling species of the Anopheles gambiae Giles complex (Dipt., Culicidae). III. The distribution, ecology, behaviour and vectorial importance of species D in Bwamba County, Uganda, with an analysis of biological, ecological, morphological and cytogenetical relationships of Ugandan species D. Bulletin of Entomological Research , 63 (1): 65-97..

Xu C H, Yu W C. 2016. Artificial chromosomes in Rice ( Oryza sativa ). Current Protocols in Plant Biology , 1 (1): 107-120..

Yabu H, Yasui H. 1991. Chromosome number in four species of Laminaria (Phaeophyta). Japanese Journal of Phycology , 39 : 185-187..

Zhang J H, Huo Y Z, Wu H L, Yu K F, Kim J K, Yarish C, Qin Y T, Liu C C, Xu R, He P M. 2014. The origin of the Ulva macroalgal blooms in the Yellow Sea in 2013. Marine Pollution Bulletin , 89 (1-2): 276-283..

Zhang J H, Liu C C, Yang L L, Gao S, Ji X, Huo Y Z, Yu K F, Xu R, He P M. 2015. The source of the Ulva blooms in the East China Sea by the combination of morphological, molecular and numerical analysis. Estuarine , Coastal and Shelf Science , 164 : 418-424..

Zhao H J, Zhang B, Chen A P, Li P Y, Xiao Y. 2008. Effects of different enzymolysis conditions on the cotyledons protoplast isolation of Alfalfa . Acta Agrestia Sinica , 16 (1): 50-53. (in Chinese with English abstract).

Zhou L J, Wang L K, Zhang J H, Cai C E, He P M. 2016. Complete mitochondrial genome of Ulva linza , one of the causal species of green macroalgal blooms in Yellow Sea, China. Mitochondrial DNA Part B , 1 (1): 31-33..

Views

Downloads

CSCD

Alert me when the article has been cited

Submit

Tools

Publicity Resources

Karyological analysis of the sea cicada Blepharipoda liberate Shen from the Rizhao intertidal zone, China

Karyotype analysis of four nemertean species

Comparative chloroplast genomes of Ulva prolifera and U. linza (Ulvophyceae) provide genetic resources for the development of interspecific markers

Chromosomal mapping of 5S and 18S-5.8S-25S rRNA genes in Saccharina japonica (Phaeophyceae) as visualized by dual-color fluorescence in situ hybridization

Preliminary study on chromosomes of induced triploid in the red sea bream,Pagrosomus major

Related Author

Liqing ZHOU

Qing ZHAO

Gaowei ZHANG

Zhihong LIU

Aiguo YANG

Xiujun SUN

Biao WU

Xuemei WANG

Related Institution

Key Laboratory of Sustainable Development of Marine Fisheries, Ministry of Agriculture, Yellow Sea Fisheries Institute, Chinese Academy of Fishery Sciences

Rizhao Ocean & Fisheries Research Institute

Laboratory for Marine Fisheries Science and Food Production Processes, Qingdao National Laboratory for Marine Science and Technology

Mariculture Research Lab Ocean University of China

College of Life Science, Qingdao University

Address：Editorial Office of JOL, 88 Haijun Rd. Qingdao, 266000, China
Tel：+86-532-82898754 Email：jol@qdio.ac.cn
Technical support is provided by Beijing Founder electronics co., LTD 京ICP备09064830号-19 京公网安备11010802024621
It is recommended to read the content of this site in Chrome&IE9+. Please switch to extreme mode in browser 360.
Cookies We use cookies to help provide and enhance our service and tailor content. By continuing, you agree to the use of cookies.

⁰

Karyological observations of Ulva linza chromosomes

DOI：10.1007/s00343-020-9105-x

摘要

Abstract

关键词

Keywords

references