Evidence for the role of KIF17 in fish spermatid reshaping: expression pattern of KIF17 in <italic style="font-style: italic">Larimichthys polyactis</italic> spermiogenesis

Jingqian WANG; Xinming GAO; Xuebin ZHENG; Chen DU; Congcong HOU; Qingping XIE; Bao LOU; Feng LIU; Shan JIN; Junquan ZHU

doi:10.1007/s00343-020-0304-2

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Evidence for the role of KIF17 in fish spermatid reshaping: expression pattern of KIF17 in Larimichthys polyactis spermiogenesis

Biology | Updated：2023-05-20

- Evidence for the role of KIF17 in fish spermatid reshaping: expression pattern of KIF17 in Larimichthys polyactis spermiogenesis
- Journal of Oceanology and Limnology Vol. 39, Issue 6, Pages: 2322-2335(2021)
- Affiliations：
  
  1.Key Laboratory of Applied Marine Biotechnology by the Ministry of Education, School of Marine Sciences, Ningbo University, Ningbo 315211, China
  2.Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, China
- Author bio：
  
  Junquan ZHU, E-mail: zhujunquan999@163.com
- Funds：
- DOI：10.1007/s00343-020-0304-2
  CLC：
- Received：23 August 2020，
  
  Accepted：27 October 2020，
  
  Online First：26 November 2020，
  
  Published：2021-11
- Accepted：
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Jingqian WANG, Xinming GAO, Xuebin ZHENG, et al. Evidence for the role of KIF17 in fish spermatid reshaping: expression pattern of KIF17 in Larimichthys polyactis spermiogenesis[J]. Journal of Oceanology and Limnology, 2021, 39(6): 2322-2335.
DOI：

Jingqian WANG, Xinming GAO, Xuebin ZHENG, et al. Evidence for the role of KIF17 in fish spermatid reshaping: expression pattern of KIF17 in Larimichthys polyactis spermiogenesis[J]. Journal of Oceanology and Limnology, 2021, 39(6): 2322-2335. DOI： 10.1007/s00343-020-0304-2.

摘要

Abstract

The homodimeric kinesin-2 protein KIF17 functions in intracellular transport and spermiogenesis in mammals. However

its role in fish spermiogenesis has not been reported. Here

we aimed to clone full-length

kif17

cDNA and determine the molecular characteristics and expression patterns of KIF17 in

Larimichthys polyactis

spermiogenesis. The full-length cDNA of

polyactis kif17

(

kif17

) was sequenced and found to contain a 332-bp 5' untranslated region

480-bp 3' untranslated region

and 2 433-bp open reading frame encoding 810 amino acids. Bioinformatics analyses showed that

polyactis

KIF17 (

-KIF17) shared high sequence similarity with homologs in other animals and possessed an N-terminal motor domain with microtubule-binding sites and adenosine triphosphate (ATP) hydrolysis sites

a stalk domain containing two coiled-coil regions

and a C-terminal tail domain. The

kif17

mRNA was widely expressed in various tissues

with the highest level in the brain

followed by that in the testis. Fluorescence in situ hybridization (FISH) analysis revealed that

kif17

was continuously expressed in spermiogenesis

showing that it had potential functions in this process. Using immunofluorescence (IF) analysis

we found that

-KIF17 colocalized with tubulin and was transferred from the perinuclear cytoplasm to the side of spermatid where the tail forms during spermiogenesis. These findings suggested that KIF17 is involved in

polyactis

spermiogenesis. In particular

it may participate in nuclear shaping and tail formation by interacting with perinuclear microtubules during spermatid reshaping. In addition to providing evidence for the role of KIF17 in fish spermatid reshaping

this study provides important data for studies of reproductive biology in

polyactis

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Keywords

references

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Related Author

Junquan ZHU

Daojun TANG

Xinming GAO

Binbin FENG

Chen DU

Congcong HOU

Shan JIN

Tao JIANG

Related Institution

Key Laboratory of Applied Marine Biotechnology by the Ministry of Education and Key Laboratory of Marine Biotechnology of Zhejiang Province, School of Marine Sciences, Ningbo University

College of Ecology, Lishui University

College of Marine Sciences, Shanghai Ocean University

Jiangsu Marine Fisheries Research Institute

Key Laboratory of Fishery Ecological Environment Assessment and Resource Conservation in Middle and Lower Reaches of Yangtze River, Freshwater Fisheries Research Center, Chinese Academy of Fishery Sciences

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Evidence for the role of KIF17 in fish spermatid reshaping: expression pattern of KIF17 in Larimichthys polyactis spermiogenesis

DOI：10.1007/s00343-020-0304-2

摘要

Abstract

关键词

Keywords

references