Relationship between morphospecies and microcystinproducing genotypes of <italic style="font-style: italic">Microcystis</italic> species in Chinese freshwaters

Yang LIU; Youxin CHEN; Haiyan FANG; Hanyang LU; Xingqiang WU; Gongliang YU; Shin-ichi NAKANO; Renhui LI

doi:10.1007/s00343-020-0276-2

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Relationship between morphospecies and microcystinproducing genotypes of Microcystis species in Chinese freshwaters

Ecology | Updated：2023-04-27

- Relationship between morphospecies and microcystinproducing genotypes of Microcystis species in Chinese freshwaters
- Journal of Oceanology and Limnology Vol. 39, Issue 5, Pages: 1926-1937(2021)
- Affiliations：
  
  1.College of Life Science, Henan Normal University, Xinxiang 453007, China
  2.Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan 430072, China
  3.Center for Ecological Research, Kyoto University, Shiga 520-2113, Japan
  4.College of Life and Environmental Sciences, Wenzhou University, Wenzhou 325000, China
  5.Journal of Henan Normal University, Xinxiang 453007, China
- Author bio：
  
  Renhui LI, E-mail: renhui.li@wzu.edu.cn, reli@ihb.ac.cn
- Funds：
- DOI：10.1007/s00343-020-0276-2
  CLC：
- Received：22 July 2020，
  
  Accepted：29 September 2020，
  
  Online First：10 November 2020，
  
  Published：2021-09
- Accepted：
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Yang LIU, Youxin CHEN, Haiyan FANG, et al. Relationship between morphospecies and microcystinproducing genotypes of Microcystis species in Chinese freshwaters[J]. Journal of Oceanology and Limnology, 2021, 39(5): 1926-1937.
DOI：

Yang LIU, Youxin CHEN, Haiyan FANG, et al. Relationship between morphospecies and microcystinproducing genotypes of Microcystis species in Chinese freshwaters[J]. Journal of Oceanology and Limnology, 2021, 39(5): 1926-1937. DOI： 10.1007/s00343-020-0276-2.

摘要

Abstract

Twenty water bodies in China were sampled

and 186 strains of different

Microcystis

species were isolated

from which eight morphospecies were identified and 43 stains containing the

mcyB

gene were detected. Phylogenetic analysis based on the

mcyB

gene indicated that the microcystin (MC)-producing

Microcystis

in China could be divided into two groups (I and II) and showed significant differences between the two groups. The maximum sequence similarity was 69.1%. Microcystins (MCs) were measured by high-performance liquid chromatography (HPLC) analysis

and no microcystin-RR (MC-RR) was detected in some strains belonging to Group II. Compared to other regions of the world

the proportion of Chinese MC-producing

Microcystis

was different

and the regional differences were more obvious. A whole-cell polymerase chain reactio (PCR) assay was conducted to analyze the proportion of the

mcyB

gene in the laboratory cultured and field cultured

Microcystis

. The proportion of four morphospecies (

M. vividis

M. ichthyoblabe

M. novacekii

and

M. aeruginosa

) that contained the

mcyB

gene exceeded 50% in the field cultured samples. Compared with former studies

M. aeruginosa

was the most likely morphotype that can produce MCs in the world. This study provided new insight of

Microcystis

hazard assessment and field monitoring.

关键词

Keywords

references

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⁰

Relationship between morphospecies and microcystinproducing genotypes of Microcystis species in Chinese freshwaters

DOI：10.1007/s00343-020-0276-2

摘要

Abstract

关键词

Keywords

references