Effect of fasting on protein metabolism in muscle tissue of <italic style="font-style: italic">Larimichthys crocea</italic> revealed by transcriptome and proteome

Yu ZHANG; Weiliang SHEN; Juan LI; Baoxiao ZHENG; Zhengjia LOU; Mohammad Aslam HOSAIN; Baoying QIAN; Liangyi XUE

doi:10.1007/s00343-020-0273-5

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Effect of fasting on protein metabolism in muscle tissue of Larimichthys crocea revealed by transcriptome and proteome

Biology | Updated：2023-04-27

- Effect of fasting on protein metabolism in muscle tissue of Larimichthys crocea revealed by transcriptome and proteome
- Journal of Oceanology and Limnology Vol. 39, Issue 5, Pages: 1972-1984(2021)
- Affiliations：
  
  1.College of Marine Sciences, Ningbo University, Ningbo 315832, China
  2.Ningbo Institute of Marine and Fisheries, Ningbo 315103, China
  3.School of Life Science, Taizhou University, Taizhou 318000, China
- Author bio：
  
  Liangyi XUE, E-mail: xueliangyi@nbu.edu.cn
- Funds：
- DOI：10.1007/s00343-020-0273-5
  CLC：
- Received：23 July 2020，
  
  Accepted：28 September 2020，
  
  Online First：11 November 2020，
  
  Published：2021-09
- Accepted：
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Yu ZHANG, Weiliang SHEN, Juan LI, et al. Effect of fasting on protein metabolism in muscle tissue of Larimichthys crocea revealed by transcriptome and proteome[J]. Journal of Oceanology and Limnology, 2021, 39(5): 1972-1984.
DOI：

Yu ZHANG, Weiliang SHEN, Juan LI, et al. Effect of fasting on protein metabolism in muscle tissue of Larimichthys crocea revealed by transcriptome and proteome[J]. Journal of Oceanology and Limnology, 2021, 39(5): 1972-1984. DOI： 10.1007/s00343-020-0273-5.

摘要

Abstract

The large yellow croaker (

Larimichthys crocea

) is an important mariculture fish in China. Farmed large yellow croaker undergo periods of fasting to adapt to the environment or to improve meat quality. To better understand the physiological responses of their muscle tissues to fasting stresses

we analyzed the transcriptomes and proteomes of both normally-fed and fasting fish groups and identified 7 578 differentially expressed genes (DEGs) and 297 differentially expressed proteins (DEPs) among them. Gene ontology and KEGG analysis showed that the enriched biological pathways were mainly involved in various synthetic and catabolic pathways

especially the protein metabolism. Based on the omics data

nine DEGs related to muscle composition (

CAN3

MYL3

and

TNNC2

)

growth (

MSTN

and

MYF5

)

autophagy (

TSC2

and

ULK1

)

and the ubiquitin proteasome pathway (

PRS6B

and

UCHL3

) were examined using qPCR. In response to fasting stress

MYL3

and

TNNC2

were significantly downregulated

while genes associated with autophagy and the ubiquitin proteasome pathway were significantly upregulated. In response to fasting stress

MYL3

TNNC2

and

MYF5

positively correlated with muscle growth were significantly downregulated

while inhibiting growth

MSTN

and genes associated with autophagy and the ubiquitin proteasome pathways were significantly upregulated. These results clarify the effects of fasting on metabolic changes in their muscle components and growth at the molecular level.

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Keywords

references

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Hailun FU

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School of Marine Science and Engineering, Qingdao Agricultural University

State Key Laboratory of Breeding Biotechnology and Sustainable Aquaculture, Institute of Oceanology, Chinese Academy of Sciences

Laboratory for Marine Biology and Biotechnology, Qingdao Marine Science and Technology Center

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College of Marine Science, University of Chinese Academy of Sciences

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Effect of fasting on protein metabolism in muscle tissue of Larimichthys crocea revealed by transcriptome and proteome

DOI：10.1007/s00343-020-0273-5

摘要

Abstract

关键词

Keywords

references