Observation and analysis of morphology abnormalities in development of<italic style="font-style: italic">Oryzias melastigma</italic>embryos

Jingjing SONG; Haiyi WU; Kaikai LIU; Wendan CHI; Shanshan GE; Zhipeng ZHANG; Mingqi WANG; Yi YU; Daode YU

doi:10.1007/s00343-020-0227-y

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Observation and analysis of morphology abnormalities in development ofOryzias melastigmaembryos

Biology | Updated：2023-05-20

- Observation and analysis of morphology abnormalities in development ofOryzias melastigmaembryos
- Journal of Oceanology and Limnology Vol. 39, Issue 4, Pages: 1485-1499(2021)
- Affiliations：
  
  1.Healthy Mariculture Engineering Research Center of Shandong Province, Marine Science Research Institute of Shandong Province, Qingdao 266104, China
  2.Tianjin Research Institute for Water Transport Engineering, Ministry of Transport, Tianjin 300456, China
  3.Ocean University of China, Qingdao 266100, China
- Author bio：
  
  Daode YU, E-mail:yudaode@shandong.cn
- Funds：
- DOI：10.1007/s00343-020-0227-y
  CLC：
- Received：10 June 2020，
  
  Accepted：02 August 2020，
  
  Online First：23 September 2020，
  
  Published：2021-07
- Accepted：
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Jingjing SONG, Haiyi WU, Kaikai LIU, et al. Observation and analysis of morphology abnormalities in development ofOryzias melastigmaembryos[J]. Journal of Oceanology and Limnology, 2021, 39(4): 1485-1499.
DOI：

Jingjing SONG, Haiyi WU, Kaikai LIU, et al. Observation and analysis of morphology abnormalities in development ofOryzias melastigmaembryos[J]. Journal of Oceanology and Limnology, 2021, 39(4): 1485-1499. DOI： 10.1007/s00343-020-0227-y.

摘要

Abstract

Fish embryos are widely used as models in toxicology

drug development

and human disease research because of their high sensitivity

observability

and operability

providing the basis for an in-depth understanding of the embryogenesis. Increasing studies have indicated that birth defects are hereditary. In this study

we used

Oryzias melastigma

as a model to conduct a study of 185-day embryogenesis and observed self-induced non-pathological abnormal embryogenesis.

O. melastigma

experienced pre-puberty stage

adolescence stage

and senescence stage

and individuals produced up to 102 eggs per day. However

the fecundity was markedly reduced during the senescent stage. During the active egg and blastodisc stages

pseudo-fertilization and pseudo-blastocysts were observed. During cleavage at the 4-to 32-cell stages

we observed blastomeres separation or dislocation. Excessively separated blastomeres formed double blastoderms

eventually resulting in conjoined twins. During the blastula stage

we observed abnormally increased cell volume

narrowed and elongated blastocysts

and abnormally coated blastoderms. At the organogenesis stage

we observed abnormal numbers of Kupffer's vesicles and conjoined twins. Abnormality in the location and number of oil droplets were observed in various development stages. Abnormal development was more commonly observed in fertilized eggs produced by broodstock in prepuberty or senescence stages

which is probably related to the age of fish and the egg quality. This study can provide the materials for comparative analysis in toxicological and molecular studies of

O. melastigma

and may provide evidence for other economic fish that produce sticky eggs.

关键词

Keywords

references

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⁰

Observation and analysis of morphology abnormalities in development ofOryzias melastigmaembryos

DOI：10.1007/s00343-020-0227-y

摘要

Abstract

关键词

Keywords

references