<italic style="font-style: italic">Karlodinium elegans</italic> sp. nov. (Gymnodiniales, Dinophyceae), a novel species isolated from the East China Sea in a dinoflagellate bloom

Jingyi CEN; Jianyan WANG; Lifen HUANG; Yarou LIN; Guangmao DING; Yuzao QI; Songhui LÜ

doi:10.1007/s00343-020-0221-4

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Karlodinium elegans sp. nov. (Gymnodiniales, Dinophyceae), a novel species isolated from the East China Sea in a dinoflagellate bloom

Biology | Updated：2023-05-20

- Karlodinium elegans sp. nov. (Gymnodiniales, Dinophyceae), a novel species isolated from the East China Sea in a dinoflagellate bloom
- Journal of Oceanology and Limnology Vol. 39, Issue 1, Pages: 242-258(2021)
- Affiliations：
  
  1.Research Center of Harmful Algae and Marine Biology, Jinan University, Guangzhou 510632, China
  2.Southern Marine Science and Engineering Guangdong Laboratory, Zhuhai 519000, China
  3.Department of Science Research, Beijing Museum of Natural History, Beijing 100050, China
  4.Fujian Fishery Resources Monitoring Center, Fuzhou 350003, China
  5.Key Laboratory of Eutrophication and Red Tide Prevention of Guangdong Higher Education Institutes, Jinan University, Guangzhou 510632, China
- Author bio：
  
  jianyan WANG, E-mail:wjy198530@163.com
  Songhui LÜ, E-mail:lusonghui1963@163.com
- Funds：
- DOI：10.1007/s00343-020-0221-4
  CLC：
- Received：08 June 2020，
  
  Accepted：25 July 2020，
  
  Online First：25 August 2020，
  
  Published：2021-01
- Accepted：
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Jingyi CEN, Jianyan WANG, Lifen HUANG, et al. Karlodinium elegans sp. nov. (Gymnodiniales, Dinophyceae), a novel species isolated from the East China Sea in a dinoflagellate bloom[J]. Journal of Oceanology and Limnology, 2021, 39(1): 242-258.
DOI：

Jingyi CEN, Jianyan WANG, Lifen HUANG, et al. Karlodinium elegans sp. nov. (Gymnodiniales, Dinophyceae), a novel species isolated from the East China Sea in a dinoflagellate bloom[J]. Journal of Oceanology and Limnology, 2021, 39(1): 242-258. DOI： 10.1007/s00343-020-0221-4.

摘要

Abstract

On May 24-29

2019

a harmful algal bloom occurred in Pingtan coastal areas

Fujian

southeast China

and caused mass mortality of cage-cultured fish. Two clonal cultures of an unknown naked dinoflagellate were set up from seawater samples taken during the bloom. The cultures were examined for morphological features

ultrastructure characters

photosynthetic pigments

the large subunit (LSU) of the rRNA gene

and the internal transcribed spacer (ITS) sequences

as well as acute toxicity analysis. The cell was unarmored

small-sized

and ovoid

and was characterized by elaborate striations on the epicone and hypocone. The nucleus was large

ellipsoid to oval or kidney-shaped

and centrally located in the cell. A long linear apical groove originated above the sulcus in the ventral epicone and extended to the dorsal side. An elongate

slit-like "ventral pore" was located on the left of the epicone

well away from the apical groove. The chloroplasts were yellowish brown

numerous

band like

and irregularly distributed in the cell periphery. Fucoxanthin was the main accessory pigment composition. Phylogeny topology reconstructed on partial LSU rDNA showed that the unknown dinoflagellate branched as a sister species to

Karlodinium

sp. (strain IFR981

IFR797

from France) and

Karlodinium corrugatum

(strain KDGSO08

from Australia)

with genetic divergences of 0.6% and 3.3%

respectively. Based on the morphological and molecular phylogenetic analysis

we describe the novel dinoflagellate as

Karlodinium elegans

sp. nov. A toxicity assay revealed that the clonal culture of

K. elegans

(strain PTB601) had no adverse effect on brine shrimp (

Artemia

salina

) and marine medaka (

Oryzias melastigma

)

indicating it may not be a toxic species.

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Keywords

references

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Karlodinium elegans sp. nov. (Gymnodiniales, Dinophyceae), a novel species isolated from the East China Sea in a dinoflagellate bloom

DOI：10.1007/s00343-020-0221-4

摘要

Abstract

关键词

Keywords

references