C<sub>17</sub>-fengycin B, produced by deep-sea-derived <italic style="font-style: italic">Bacillus subtilis</italic>, possessing a strong antifungal activity against <italic style="font-style: italic">Fusarium solani</italic>

Weixiang LIU; Chaomin SUN

doi:10.1007/s00343-020-0215-2

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C₁₇-fengycin B, produced by deep-sea-derived Bacillus subtilis, possessing a strong antifungal activity against Fusarium solani

Biology | Updated：2023-04-27

- C₁₇-fengycin B, produced by deep-sea-derived Bacillus subtilis, possessing a strong antifungal activity against Fusarium solani
- Journal of Oceanology and Limnology Vol. 39, Issue 5, Pages: 1938-1947(2021)
- Affiliations：
  
  1.CAS and Shandong Province Key Laboratory of Experimental Marine Biology, Institute of Oceanology, Center for Ocean MegaScience, Chinese Academy of Sciences, Qingdao 266071, China
  2.Laboratory for Marine Biology and Biotechnology, Pilot National Laboratory for Marine Science and Technology (Qingdao), Qingdao 266237, China
- Author bio：
  
  Chaomin SUN, E-mail: sunchaomin@qdio.ac.cn
- Funds：
- DOI：10.1007/s00343-020-0215-2
  CLC：
- Received：02 June 2020，
  
  Accepted：22 September 2020，
  
  Online First：08 October 2020，
  
  Published：2021-09
- Accepted：
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Weixiang LIU, Chaomin SUN. C₁₇-fengycin B, produced by deep-sea-derived Bacillus subtilis, possessing a strong antifungal activity against Fusarium solani[J]. Journal of Oceanology and Limnology, 2021, 39(5): 1938-1947.
DOI：

Weixiang LIU, Chaomin SUN. C₁₇-fengycin B, produced by deep-sea-derived Bacillus subtilis, possessing a strong antifungal activity against Fusarium solani[J]. Journal of Oceanology and Limnology, 2021, 39(5): 1938-1947. DOI： 10.1007/s00343-020-0215-2.

摘要

Abstract

Root rot disease caused by

Fusarium solani

is the most devastating disease of the tomato and legume crops in China. The metabolites of

Bacillus

species can inhibit many fungal diseases. In this study

the metabolites of deep-sea-derived bacterium

Bacillus subtilis

2H11 can significantly inhibit the growth of

F. solani

. The metabolite C

-fengycin B

one of the cyclic lipopeptides

was identified by the combination of silica column chromatography

high-performance liquid chromatography (HPLC)

high-energy collision induced dissociation mass spectrometry (HCD-MS) and tandem mass spectrometry (HCD-MS/MS). The results of scanning electron microscopy (SEM) and transmission electron microscopy (TEM) showed that C

-fengycin B could destroy the structure of the hyphae and spores of

F. solani

. The antifungal activities of C

-fengycin B against

F. solani

were tested at concentrations ranging from 0.05 mg/mL to 0.20 mg/mL. The results indicated that C

-fengycin B inhibited the growth of

F. solani

with antifungal index of 89.80% at 0.20 mg/mL

and the antifungal activity of C

-fengycin B was further verified by the pot experiment. In addition

the cytotoxicity experiment showed that C

-fengycin B had good biocompatibility and was a potential candidate for the development of biocontrol pesticide in the future.

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C17-fengycin B, produced by deep-sea-derived Bacillus subtilis, possessing a strong antifungal activity against Fusarium solani

DOI：10.1007/s00343-020-0215-2

摘要

Abstract

关键词

Keywords

references

C₁₇-fengycin B, produced by deep-sea-derived Bacillus subtilis, possessing a strong antifungal activity against Fusarium solani