Comparison of fungal community composition within different intestinal segments of tilapia and bighead carp

Lei ZHOU; Yaoquan HAN; Dapeng WANG; Yusen LI; Xiande HUANG; Anyou HE

doi:10.1007/s00343-020-0214-3

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Comparison of fungal community composition within different intestinal segments of tilapia and bighead carp

Biology | Updated：2023-04-27

- Comparison of fungal community composition within different intestinal segments of tilapia and bighead carp
- Journal of Oceanology and Limnology Vol. 39, Issue 5, Pages: 1961-1971(2021)
- Affiliations：
  
  1.Guangxi Key Laboratory of Aquatic Genetic Breeding and Healthy Aquaculture, Guangxi Academy of Fishery Sciences, Nanning 530021, China
  2.Joint Laboratory of Guangdong Province and Hong Kong Region on Marine Bioresource Conservation and Exploitation, College of Marine Sciences, South China Agricultural University, Guangzhou 510642, China
- Author bio：
  
  Xiande HUANG, E-mail: huangxd@scau.edu.cn
  Anyou HE, E-mail: heanyou2000@aliyun.com
- Funds：
- DOI：10.1007/s00343-020-0214-3
  CLC：
- Received：30 May 2020，
  
  Accepted：12 October 2020，
  
  Online First：06 November 2020，
  
  Published：2021-09
- Accepted：
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Lei ZHOU, Yaoquan HAN, Dapeng WANG, et al. Comparison of fungal community composition within different intestinal segments of tilapia and bighead carp[J]. Journal of Oceanology and Limnology, 2021, 39(5): 1961-1971.
DOI：

Lei ZHOU, Yaoquan HAN, Dapeng WANG, et al. Comparison of fungal community composition within different intestinal segments of tilapia and bighead carp[J]. Journal of Oceanology and Limnology, 2021, 39(5): 1961-1971. DOI： 10.1007/s00343-020-0214-3.

摘要

Abstract

Although intestinal fungi play important roles in host health and disease

the composition and diversity of fungal communities remain poorly reported in fish. In this study

fungi in the fore-

mid-

and hindintestine of tilapia (

Oreochromis mossambicus

) and bighead carp (

Aristichthys nobilis

) from Hongchaojiang Reservoir in Guangxi

China were investigated by ITS sequencing. Based on this

we obtained 1 763 478 high-quality tags

which clustered into 1 089 operational taxonomic units (OTUs). In total

404 OTUs were annotated

of which 310

and 26 belonged to Ascomycota

Basidiomycota

and other

respectively. Results show significant differences in the community composition of intestinal fungi between tilapia and bighead carp but not within their different intestinal segments. Furthermore

154 of the 404 annotated OTUs were considered reliable and were classified into three trophic modes and nine guilds. The three trophic modes consisted of 108 OTUs of saprotrophic fungi

41 OTUs of pathotrophic fungi

and five OTUs of symbiotrophic fungi. The top three most abundant OTUs overall (i.e.

Otu000002

Scopulariopsis acremonium

; Otu000018

Alternaria palandui

; Otu000034

Aureobasidium pullulans

) showed lower abundance in the hind-intestinal segments of bighead carp

suggesting uneven distribution of these fungi in this species. In addition

saprotrophic and pathotrophic fungi were markedly decreased in the hindintestine. It is indicated that the fungal community was not only related to host species specificity but also to the respective physiological functions of different intestinal segments. These findings provide valuable information on the composition

structure

and potential function of the intestinal fungi community associated with different intestinal segments in tilapia and bighead carp under natural conditions

thus highlighting the importance of fungi as an integral part of the intestinal microbiota in maintaining host health.

关键词

Keywords

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