Triterpenoid saponin biosynthesis genes and their expression patterns during the development of sea cucumber <italic style="font-style: italic">Apostichopus japonicus</italic>

Yujia YANG; Xiaoni LI; Lina SUN

doi:10.1007/s00343-020-0209-0

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Triterpenoid saponin biosynthesis genes and their expression patterns during the development of sea cucumber Apostichopus japonicus

Biology | Updated：2023-05-20

- Triterpenoid saponin biosynthesis genes and their expression patterns during the development of sea cucumber Apostichopus japonicus
- Journal of Oceanology and Limnology Vol. 39, Issue 6, Pages: 2295-2308(2021)
- Affiliations：
  
  1.Laboratory for Evolution and Development, Institute of Evolution and Marine Biodiversity, Ocean University of China, Qingdao 266003, China
  2.CAS Key Laboratory of Marine Ecology and Environmental Sciences, Institute of Oceanology, Chinese Academy of Sciences, Qingdao 266071, China
- Author bio：
  
  Lina SUN, E-mail: sunlina@qdio.ac.cn
- Funds：
- DOI：10.1007/s00343-020-0209-0
  CLC：
- Received：31 May 2020，
  
  Accepted：25 July 2020，
  
  Online First：24 November 2020，
  
  Published：2021-11
- Accepted：
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Yujia YANG, Xiaoni LI, Lina SUN. Triterpenoid saponin biosynthesis genes and their expression patterns during the development of sea cucumber Apostichopus japonicus[J]. Journal of Oceanology and Limnology, 2021, 39(6): 2295-2308.
DOI：

Yujia YANG, Xiaoni LI, Lina SUN. Triterpenoid saponin biosynthesis genes and their expression patterns during the development of sea cucumber Apostichopus japonicus[J]. Journal of Oceanology and Limnology, 2021, 39(6): 2295-2308. DOI： 10.1007/s00343-020-0209-0.

摘要

Abstract

Sea cucumbers are echinoderm species with a leathery skin and an elongated body. Natural compounds

including saponins

have been previously isolated from sea cucumber. The majority of saponins derived from this organism typically belong to the triterpene glycosides

which exhibit various biological effects (e.g.

antifungal

cytotoxic

hemolytic

and cytostatic immunomodulatory activities). In the present study

30 saponin biosynthesis enzymes

namely 2 AACTs

2 FPSs

2 HMGSs

2 OSCs

1 SS

2 SEs

and 19 UGTs

were found in the genome of sea cucumber

Apostichopus japonicus

. Compared with upstream saponin biosynthesis enzymes (i.e.

AACTs

FPSs

HMGSs

OSCs

and SSs)

the downstream saponin biosynthesis enzymes (SEs and UGTs) shared lower amino acid sequence similarity with the corresponding genes in other echinoderms. It was proposed that the enzyme structures comprised both α-helices and β-sheets. Notably

only a low percentage of β-sheets were present in FPSs

OSCs

and SS. The saponin biosynthesis enzymes showed dynamic expression patterns during five critical developmental stages of

japonicus

(fertilized oocytes

blastula

gastrula

doliolaria

and penractula). The present study involved elucidation of putative saponin biosynthesis pathways in sea cucumber and provides a valuable platform for further investigation of saponin biosynthesis in echinoderms.

关键词

Keywords

references

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Triterpenoid saponin biosynthesis genes and their expression patterns during the development of sea cucumber Apostichopus japonicus

DOI：10.1007/s00343-020-0209-0

摘要

Abstract

关键词

Keywords

references