Isolation and identification of antimicrobial metabolites from sea anemone-derived fungus <italic style="font-style: italic">Emericella</italic> sp. SMA01

Yang YUE; Huahua YU; Rongfeng LI; Linfeng HU; Song LIU; Rong'e XING; Pengcheng LI

doi:10.1007/s00343-020-0203-6

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Isolation and identification of antimicrobial metabolites from sea anemone-derived fungus Emericella sp. SMA01

Biology | Updated：2023-05-20

- Isolation and identification of antimicrobial metabolites from sea anemone-derived fungus Emericella sp. SMA01
- Journal of Oceanology and Limnology Vol. 39, Issue 3, Pages: 1010-1019(2021)
- Affiliations：
  
  1.Key Laboratory of Experimental Marine Biology, Center for Ocean Mega-Science, Institute of Oceanology, Chinese Academy of Sciences, Qingdao 266071, China
  2.Laboratory for Marine Drugs and Bioproducts, Pilot Qingdao National Laboratory for Marine Science and Technology, Qingdao 266237, China
  3.School of Chemistry and Chemical Engineering, Henan Institute of Science and Technology, Xinxiang 453003, China
- Author bio：
  
  Huahua YU, E-mail: yuhuahua@qdio.ac.cn
  Pengcheng LI, E-mail: pcli@qdio.ac.cn
- Funds：
- DOI：10.1007/s00343-020-0203-6
  CLC：
- Received：19 May 2020，
  
  Accepted：14 July 2020，
  
  Online First：05 August 2020，
  
  Published：2021-05
- Accepted：
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Yang YUE, Huahua YU, Rongfeng LI, et al. Isolation and identification of antimicrobial metabolites from sea anemone-derived fungus Emericella sp. SMA01[J]. Journal of Oceanology and Limnology, 2021, 39(3): 1010-1019.
DOI：

Yang YUE, Huahua YU, Rongfeng LI, et al. Isolation and identification of antimicrobial metabolites from sea anemone-derived fungus Emericella sp. SMA01[J]. Journal of Oceanology and Limnology, 2021, 39(3): 1010-1019. DOI： 10.1007/s00343-020-0203-6.

摘要

Abstract

Marine symbiotic fungi represent an intriguing source of discovery of novel secondary metabolites with various biological activities. Sea anemones are benthic marine invertebrates

however

the cultivable symbiotic fungi residing in the sea anemones are paid few attentions compared to those derived from their cnidarian counterparts. Here we show the identification of antimicrobial secondary metabolites from the sea anemone-derived symbiotic fungi. Out of five isolated fungal strains

only the strain SMA01 showed strong antimicrobial activities

which was assigned into the genus

Emericella

based on the morphological characteristics and the ITS sequencing. Media swift from liquid fermentation to solid rice medium presented little influence on its antibacterial activity. A chemical investigation of the ethyl acetate extract of the

Emericella

sp. SMA01 led to discovery of the primary antibiotic metabolite phenazine-1-carboxylic acid. The IC

values of the phenazine-1-carboxylic acid against

Phytophthora capsici

Gibberella

zeae

and

Verticillium dahliae

were determined to be 23.26-53.89 μg/mL. To the best of our knowledge

this was the first report of

Emericella

sp. in sea anemones. The current study may benefit understanding of the defensive chemical interactions between the symbiotic fungi and their host sea anemones.

关键词

Keywords

references

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Related Institution

CAS and Shandong Province Key Laboratory of Experimental Marine Biology, Institute of Oceanology, Chinese Academy of Sciences

College of Marine Science, University of Chinese Academy of Sciences

Laboratory of Marine Biology and Biotechnology, Pilot National Laboratory for Marine Science and Technology

Center for Ocean Mega-Science, Chinese Academy of Sciences

Key Laboratory of Experimental Marine Biology, Institute of Oceanology, Chinese Academy of Sciences, and Laboratory of Marine Biology and Biotechnology, Qingdao National Laboratory for Marine Science and Technology

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Isolation and identification of antimicrobial metabolites from sea anemone-derived fungus Emericella sp. SMA01

DOI：10.1007/s00343-020-0203-6

摘要

Abstract

关键词

Keywords

references