On microbial community of <italic style="font-style: italic">Pyropia haitanensis</italic> by metagenomic analysis

Junhao WANG; Yunxiang MAO; Guoying DU; Xiaojiao LI; Xianghai TANG

doi:10.1007/s00343-020-0189-0

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On microbial community of Pyropia haitanensis by metagenomic analysis

Aquaculture and Fisheries | Updated：2023-05-20

- On microbial community of Pyropia haitanensis by metagenomic analysis
- Journal of Oceanology and Limnology Vol. 39, Issue 3, Pages: 1091-1102(2021)
- Affiliations：
  
  1.Key Laboratory of Marine Genetics and Breeding(Ministry of Education), College of Marine Life Sciences, Ocean University of China, Qingdao 266003, China
  2.Key Laboratory of Utilization and Conservation of Tropical Marine Bioresource(Ministry of Education), College of Fisheries and Life Science, Hainan Tropical Ocean University, Sanya 572022, China
- Author bio：
  
  Xianghai TANG, E-mail: txianghai@ouc.edu.cn
- Funds：
- DOI：10.1007/s00343-020-0189-0
  CLC：
- Received：10 May 2020，
  
  Accepted：23 May 2020，
  
  Online First：04 June 2020，
  
  Published：2021-05
- Accepted：
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Junhao WANG, Yunxiang MAO, Guoying DU, et al. On microbial community of Pyropia haitanensis by metagenomic analysis[J]. Journal of Oceanology and Limnology, 2021, 39(3): 1091-1102.
DOI：

Junhao WANG, Yunxiang MAO, Guoying DU, et al. On microbial community of Pyropia haitanensis by metagenomic analysis[J]. Journal of Oceanology and Limnology, 2021, 39(3): 1091-1102. DOI： 10.1007/s00343-020-0189-0.

摘要

Abstract

Microorganisms plays an important role in the growth of

Pyropia haitanensis

. To understand the structural and functional diversity of the microorganism community of

haitanensis

(PH40)

the associated metabolic pathway network in cluster of orthologous groups (COG) and Kyoto Encyclopedia of Genes and Genomes (KEGG)

and carbohydrate-active enzymes (CAZymes) were explored in metagenomic analysis. DNA extraction from gametophytes of

haitanensis

was performed first

followed by library construction

sequencing

preprocessing of sequencing data

taxonomy assignment

gene prediction

and functional annotation. The results show that the predominant microorganisms of

haitanensis

were bacteria (98.98%)

and the phylum with the highest abundance was Proteobacteria (54.64%)

followed by Bacteroidetes (37.92%).

Erythrobacter

(3.98%) and

Hyunsoonleella jejuensis

(1.56%) were the genera and species with the highest abundance of bacteria

respectively. The COG annotation demonstrated that genes associated with microbial metabolism was the predominant category. The results of metabolic pathway annotation show that the ABC transport system and two-component system were the main pathways in the microbial community. Plant growth hormone biosynthesis pathway and multi-vitamin biosynthesis functional units (modules) were the other important pathways. The CAZyme annotation revealed that the starch might be an important carbon source for microorganisms. Glycosyl transferase family 2 (GT2) and glycosyl transferase family 3 (GT3) were the highly abundant families in glucoside transferase superfamily. Six metagenome-assembled genomes containing enzymes involved in the biosynthesis of cobalamin (vitamin B12) and indole-3-acetic acid were obtained by binning method. They were confirmed to belong to Rhodobacterales and Rhizobiales

respectively. Our findings provide comprehensive insights into the microorganism community of

Pyropia

关键词

Keywords

references

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On microbial community of Pyropia haitanensis by metagenomic analysis

DOI：10.1007/s00343-020-0189-0

摘要

Abstract

关键词

Keywords

references