Molecular characterization and expression of the<italic style="font-style: italic">SiUCP2</italic>gene in sea urchin<italic style="font-style: italic">Strongylocentrotus intermedius</italic>

Lingshu HAN; Zijiao QUAN; Bing HAN; Beichen DING; Xiaofang HUANG; Heng WANG; Yaqing CHANG; Jun DING

doi:10.1007/s00343-020-0181-8

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Molecular characterization and expression of theSiUCP2gene in sea urchinStrongylocentrotus intermedius

Biology | Updated：2023-05-20

- Molecular characterization and expression of theSiUCP2gene in sea urchinStrongylocentrotus intermedius
- Journal of Oceanology and Limnology Vol. 39, Issue 4, Pages: 1523-1537(2021)
- Affiliations：
  
  Key Laboratory of Mariculture & Stock Enhancement in North China's Sea, Ministry of Agriculture and Rural Affairs, Dalian Ocean University, Dalian 116023, China
- Author bio：
  
  Jun DING, E-mail:dingjun19731119@hotmail.com
- Funds：
- DOI：10.1007/s00343-020-0181-8
  CLC：
- Received：08 May 2020，
  
  Accepted：03 July 2020，
  
  Online First：01 September 2020，
  
  Published：2021-07
- Accepted：
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Lingshu HAN, Zijiao QUAN, Bing HAN, et al. Molecular characterization and expression of theSiUCP2gene in sea urchinStrongylocentrotus intermedius[J]. Journal of Oceanology and Limnology, 2021, 39(4): 1523-1537.
DOI：

Lingshu HAN, Zijiao QUAN, Bing HAN, et al. Molecular characterization and expression of theSiUCP2gene in sea urchinStrongylocentrotus intermedius[J]. Journal of Oceanology and Limnology, 2021, 39(4): 1523-1537. DOI： 10.1007/s00343-020-0181-8.

摘要

Abstract

Uncoupling protein 2 (UCP2) is a proton transporter located in the inner mitochondrial membrane

and inhibits the formation of adenosine triphosphate and reactive oxygen species by uncoupling oxidative phosphorylation. To provide a theoretical basis for the role of

SiUCP2

in lipid metabolism

a 2 341-bp full-length cDNA of

SiUCP2

from sea urchin

Strongylocentrotus intermedius

which encodes 323 amino acids (predicted MW 36.11 kDa) was obtained

and the structure and function of the

SiUCP2

gene and its expression at the mRNA and protein level were studied.

SiUCP2

had high homology with

UCP2

of other species. Expression of

SiUCP2

was detected in the order of tube feet

gonads

coelomocytes

intestines. The expression level was the highest in prismatic larvae and lowest in the two-cell stage. Moreover

using in-situ hybridization

we found that SiUCP2 protein was expressed in the gonads and intestine. This study provided a theoretical basis for subsequent studies on the role of

SiUCP2

and its regulatory mechanism in lipid metabolism

and for the improvement of gonad quality to obtain a higher economic value from sea urchins.

关键词

Keywords

references

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Molecular characterization and expression of theSiUCP2gene in sea urchinStrongylocentrotus intermedius

DOI：10.1007/s00343-020-0181-8

摘要

Abstract

关键词

Keywords

references