Identification and characterization of endosymbiosis-related immune genes in deep-sea mussels <italic style="font-style: italic">Gigantidas platifrons</italic>

Mengna LI; Hao CHEN; Minxiao WANG; Zhaoshan ZHONG; Li ZHOU; Chaolun LI

doi:10.1007/s00343-020-0040-7

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Identification and characterization of endosymbiosis-related immune genes in deep-sea mussels Gigantidas platifrons

Biology | Updated：2023-04-27

- Identification and characterization of endosymbiosis-related immune genes in deep-sea mussels Gigantidas platifrons
- Journal of Oceanology and Limnology Vol. 38, Issue 4, Pages: 1292-1303(2020)
- Affiliations：
  
  1.Center of Deep Sea Research and Key Laboratory of Marine Ecology & Environmental Sciences (CODR and KLMEES), Institute of Oceanology, Chinese Academy of Sciences, Qingdao 266071, China
  2.Laboratory for Marine Ecology and Environmental Science, Qingdao National Laboratory for Marine Science and Technology, Qingdao 266237, China
  3.Center for Ocean Mega-Science, Chinese Academy of Sciences, Qingdao 266071, China
  4.University of Chinese Academy of Sciences, Beijing 100049, China
- Author bio：
  
  LI Chaolun, lcl@qdio.ac.cn
- Funds：
- DOI：10.1007/s00343-020-0040-7
  CLC：
- Received：17 January 2020，
  
  Accepted：27 March 2020，
  
  Online First：18 May 2020，
  
  Published：2020-07
- Accepted：
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Mengna LI, Hao CHEN, Minxiao WANG, et al. Identification and characterization of endosymbiosis-related immune genes in deep-sea mussels Gigantidas platifrons[J]. Journal of Oceanology and Limnology, 2020, 38(4): 1292-1303.
DOI：

Mengna LI, Hao CHEN, Minxiao WANG, et al. Identification and characterization of endosymbiosis-related immune genes in deep-sea mussels Gigantidas platifrons[J]. Journal of Oceanology and Limnology, 2020, 38(4): 1292-1303. DOI： 10.1007/s00343-020-0040-7.

摘要

Abstract

Deep-sea mussels of the subfamily Bathymodiolinae are common and numerically dominant species widely distributed in cold seeps and hydrothermal vents. During long-time evolution

deep-sea mussels have evolved to be well adapted to the local environment of cold seeps and hydrothermal vents by various ways

especially by establishing endosymbiosis with chemotrophic bacteria. However

biological processes underlying the establishment and maintenance of symbiosis between host mussels and symbionts are largely unclear. In the present study

Gigantidas platifrons

genes possibly involved in the symbiosis with methane oxidation symbionts were identified and characterized by Lipopolysaccharide (LPS) pull-down and

in situ

hybridization. Five immune related proteins including Toll-like receptor 2 (TLR2)

integrin

vacuolar sorting protein (VSP)

matrix metalloproteinase 1 (MMP1)

and leucine-rich repeat (LRR-1) were identified by LPS pull-down assay. These five proteins were all conserved in either molecular sequences or functional domains and known to be key molecules in host immune recognition

phagocytosis

and lysosome-mediated digestion. Furthermore

in situ

hybridization of LRR-1

TLR2 and VSP genes was conducted to investigate their expression patterns in gill tissues of

platifrons

. Consequently

LRR-1

TLR2

and VSP genes were found expressed exclusively in the bacteriocytes of

platifrons

. Therefore

it was suggested that TLR2

integrin

VSP

MMP1

and LRR-1 might be crucial molecules in the symbiosis between

platifrons

and methane oxidation bacteria by participating in symbiosis-related immune processes.

关键词

Keywords

references

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Identification and characterization of endosymbiosis-related immune genes in deep-sea mussels Gigantidas platifrons

DOI：10.1007/s00343-020-0040-7

摘要

Abstract

关键词

Keywords

references