Molecular identification and population differentiation of <italic style="font-style: italic">Aurelia</italic> spp. ephyrae in sea cucumber aquaculture ponds of northern China

Saijun PENG; Qingqing LIU; Lei WANG; Tingting SUN; Tamara SHIGANOVA; Zhijun DONG

doi:10.1007/s00343-020-0022-9

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Molecular identification and population differentiation of Aurelia spp. ephyrae in sea cucumber aquaculture ponds of northern China

Biology | Updated：2023-05-20

- Molecular identification and population differentiation of Aurelia spp. ephyrae in sea cucumber aquaculture ponds of northern China
- Journal of Oceanology and Limnology Vol. 39, Issue 3, Pages: 989-996(2021)
- Affiliations：
  
  1.Muping Coastal Environment Research Station, Yantai Institute of Coastal Zone Research, Chinese Academy of Sciences, Yantai 264003, China
  2.University of Chinese Academy of Sciences, Beijing 100049, China
  3.Center for Ocean Mega-Science, Chinese Academy of Sciences, Qingdao 266071, China
  4.Shirshov Institute of Oceanology Russian Academy of Sciences, Moscow 117997 Nakhimovskyi pr., 36, Russia
- Author bio：
  
  Zhijun DONG, E-mail: zjdong@yic.ac.cn
- Funds：
- DOI：10.1007/s00343-020-0022-9
  CLC：
- Received：13 January 2020，
  
  Accepted：23 April 2020，
  
  Online First：10 June 2020，
  
  Published：2021-05
- Accepted：
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Saijun PENG, Qingqing LIU, Lei WANG, et al. Molecular identification and population differentiation of Aurelia spp. ephyrae in sea cucumber aquaculture ponds of northern China[J]. Journal of Oceanology and Limnology, 2021, 39(3): 989-996.
DOI：

Saijun PENG, Qingqing LIU, Lei WANG, et al. Molecular identification and population differentiation of Aurelia spp. ephyrae in sea cucumber aquaculture ponds of northern China[J]. Journal of Oceanology and Limnology, 2021, 39(3): 989-996. DOI： 10.1007/s00343-020-0022-9.

摘要

Abstract

Aurelia

spp. ephyrae have been reported to form blooms in sea cucumber aquaculture ponds in the Bohai and Yellow Seas. To identify the species

we carried out a genetic analysis of

Aurelia

spp. ephyrae and medusae based on mitochondrial 16S rRNA gene. Samples of four

Aurelia

sp. ephyrae populations were collected in sea cucumber aquaculture ponds and samples of four

Aurelia

sp. medusae populations were collected in coastal waters. Using a BLASTn search

we found that both the ephyrae collected in the aquaculture ponds and medusae collected in coastal waters belong to

Aurelia coerulea

. Seventeen haplotypes were recovered from the 16S rRNA gene. The overall haplotype diversity and nucleotide diversity of the 166

coerulea

individuals were 0.686% and 0.329%

respectively

indicating high haplotype diversity and low nucleotide diversity. Moreover

the haplotype diversity of ephyrae populations were generally lower than that of medusae populations with close sampling points. The genetic differentiation between ephyrae populations collected in the sea cucumber aquaculture ponds and

coerulea

medusae collected in coastal waters was not significant

suggesting the ephyrae populations in the sea cucumber culture ponds were part of the same genetic group as the medusae populations in the coastal waters. Phylogeographic analysis of the 16S rRNA region revealed that there was no significant correlation between the haplotypes and the geographic distribution of populations. Pairwise fixation index values showed significant genetic differentiation and limited gene flow between

coerulea

population of Weifang and other locations.

关键词

Keywords

references

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Molecular identification and population differentiation of Aurelia spp. ephyrae in sea cucumber aquaculture ponds of northern China

DOI：10.1007/s00343-020-0022-9

摘要

Abstract

关键词

Keywords

references