Profile and development of microsatellite primers for <italic style="font-style: italic">Acanthogobius ommaturus</italic> based on high-throughput sequencing technology

Chenyu SONG; Ziyi FENG; Chunhou LI; Zhicheng SUN; Tianxiang GAO; Na SONG; Lu LIU

doi:10.1007/s00343-019-9154-1

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Profile and development of microsatellite primers for Acanthogobius ommaturus based on high-throughput sequencing technology

Biology | Updated：2023-04-27

- Profile and development of microsatellite primers for Acanthogobius ommaturus based on high-throughput sequencing technology
- Journal of Oceanology and Limnology Vol. 38, Issue 6, Pages: 1880-1890(2020)
- Affiliations：
  
  1.Fisheries College, Ocean University of China, Qingdao 266003, China
  2.South China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Guangzhou 510300, China
  3.National Engineering Research Center for Marine Aquaculture, Zhejiang Ocean University, Zhoushan 316022, China
- Author bio：
  
  GAO Tianxiang, gaotianxiang0611@163.com
  SONG Na, songna624@163.com
- Funds：
- DOI：10.1007/s00343-019-9154-1
  CLC：
- Received：04 June 2019，
  
  Accepted：23 September 2019，
  
  Online First：11 October 2019，
  
  Published：2020-11
- Accepted：
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Chenyu SONG, Ziyi FENG, Chunhou LI, et al. Profile and development of microsatellite primers for Acanthogobius ommaturus based on high-throughput sequencing technology[J]. Journal of Oceanology and Limnology, 2020, 38(6): 1880-1890.
DOI：

Chenyu SONG, Ziyi FENG, Chunhou LI, et al. Profile and development of microsatellite primers for Acanthogobius ommaturus based on high-throughput sequencing technology[J]. Journal of Oceanology and Limnology, 2020, 38(6): 1880-1890. DOI： 10.1007/s00343-019-9154-1.

摘要

Abstract

Acanthogobius ommaturus

a fish species of the Family Gobiidae

is a marine commercial fish perched on the bottom of seawater. In this study

Illumina high-throughput sequencing technology was applied to obtain the candidate microsatellite markers of

A. ommaturus

. A total of 4 746 microsatellite-rich fragments were found

of which 4 542 microsatellites are with primer fragments

containing 971 dinucleotide sequences

2 643 trinucleotide sequences

569 tetranucleotide sequences

406 pentanucleotide sequences

and 212 hexanucleotide sequences. Based on the results of high-throughput sequencing

a total of 141 pairs of the microsatellite primers were designed and screened. And then 24 polymorphic primers were finally obtained by polyacrylamide gel electrophoresis. In total

271 alleles were detected in the 24 pairs of primers. The number of alleles for different primers ranged from 5 to 19. The average number of effective alleles (

) was 11.292; the average observed heterozygosity (

) of the 24 pairs of primers was 0.665

the average expected heterozygosity (

) was 0.880

and the average polymorphic information content was 0.846. All sites were highly polymorphic (PIC

0.50).

关键词

Keywords

references

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⁰

Profile and development of microsatellite primers for Acanthogobius ommaturus based on high-throughput sequencing technology

DOI：10.1007/s00343-019-9154-1

摘要

Abstract

关键词

Keywords

references